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Microbiology > Laboratory Tests for Enterobacteriaciae > Flashcards

Laboratory Tests for Enterobacteriaciae Flashcards

1
Q

Positive control in Oxidase Test

A

P. aeruginosa

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2
Q

Negative control in Oxidase Test

A

E. coli

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3
Q

Organisms that do not grow on Mac

A 
Haemophilus         Pasteurella
HACEK                   Legionella
Parvobacteria        Moraxella
                                Francisella
                                Brucella
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4
Q

Reagents in Nitrate Reduction Test

A

Sulfanilic Acid

Alpha-napthylamine

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5
Q

Added in Nitrate Reduction Test to confirm negative reaction

A

Zinc dust/powder

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6
Q

Positive result in nitrate reduction test

A

Red color

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7
Q

Determines whether the organism is a slow or LLF or true NLF

A

ONPG test

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8
Q

Positive result in ONPG test

A

Yellow color within 20 mins

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9
Q

Decarboxylase Test is also known as

A

Moeller’s Method

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10
Q

pH indicator in Decarboxylase Test

A

Bromcresol Purple

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11
Q

decarboxylase

Lysine ——————–>

A

Cadaverine

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12
Q

decarboxylase

Ornithine ——————–>

A

Putrescine

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13
Q

dihydrolase

Arginine ——————–>

A

Citrulline

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14
Q

Promotes anaerobiosis in decarboxylase test

A

Mineral Oil

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15
Q

Positive result in LOA test

A

Alkaline product - purple

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16
Q

Negative result in LOA test

A

Yellow color (fermentation of glucose)

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17
Q

Amino acid enzyme that requires aerobic environment

A

Deaminase

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18
Q

Decarboxylase Test is also known as

A

Moeller’s Method

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19
Q

pH indicator in Decarboxylase Test

A

Bromcresol Purple

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20
Q

decarboxylase

Lysine ——————–>

A

Cadaverine

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21
Q

decarboxylase

Ornithine ——————–>

A

Putrescine

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22
Q

dihydrolase

Arginine ——————–>

A

Citrulline

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23
Q

Promotes anaerobiosis in decarboxylase test

A

Mineral Oil

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24
Q

Positive result in LOA test

A

Alkaline product - purple

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25
Q

Negative result in LOA test

A

Yellow color (fermentation of glucose)

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26
Q

Amino acid enzyme that requires aerobic environment

A

Deaminase

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27
Q

Decarboxylase Test is also known as

A

Moeller’s Method

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28
Q

pH indicator in Decarboxylase Test

A

Bromcresol Purple

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29
Q

decarboxylase

Lysine ——————–>

A

Cadaverine

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30
Q

decarboxylase

Ornithine ——————–>

A

Putrescine

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31
Q

dihydrolase

Arginine ——————–>

A

Citrulline

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32
Q

Promotes anaerobiosis in decarboxylase test

A

Mineral Oil

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33
Q

Positive result in LOA test

A

Alkaline product - purple

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34
Q

Negative result in LOA test

A

Yellow color (fermentation of glucose)

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35
Q

Amino acid enzyme that requires aerobic environment

A

Deaminase

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36
Q

Decarboxylase Test is also known as

A

Moeller’s Method

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37
Q

pH indicator in Decarboxylase Test

A

Bromcresol Purple

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38
Q

decarboxylase

Lysine ——————–>

A

Cadaverine

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39
Q

decarboxylase

Ornithine ——————–>

A

Putrescine

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40
Q

dihydrolase

Arginine ——————–>

A

Citrulline

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41
Q

Promotes anaerobiosis in decarboxylase test

A

Mineral Oil

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42
Q

Positive result in LOA test

A

Alkaline product - purple

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43
Q

Negative result in LOA test

A

Yellow color (fermentation of glucose)

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44
Q

Amino acid enzyme that requires aerobic environment

A

Deaminase

45
Q

Ratio of glucose, lactose, sucrose in TSI

A

1:10:10

46
Q

pH indicator in TSI

A

Phenol Red

47
Q

H2S indicators in TSI

A

Ferrous sulfate

Sodium Thiosulfate

48
Q

TSI reaction when 2-3 sugars are fermented

A

A/A = LF

49
Q

TSI reaction when glucose is fermented

A

K/A =NLF

50
Q

TSI reaction when no sugar is fermented

A

K/K (Non fermentative: P. aeruginosa)

51
Q

Formation of bubbles or splitting of the media in the butt in TSI indicate

A

Gas production

52
Q

Used to determine whether a Gram-negative rod decarboxylates or deaminates lysine and forms H2S

A

LIA

53
Q

Components of LIA

A

Lysine, Peptones, 0.1% Glucose

54
Q

H2S indicators in LIA test

A

Sodium Thiosulfate

Ferric ammonium citrate

55
Q

pH indicator in LIA test

A

Bromcresol purple

56
Q

(-) Lysine Deamination

(+) Lysine Decarboxylation

A

K/K

Purple/Purple

57
Q

(-) Lysine Deamination

(-) Lysine Decarboxylation

A

K/A

Purple/Yellow

58
Q

(+) Lysine Deamination

(-) Lysine Decarboxylation

A

R/A

Red/Yellow

59
Q

Lysine deamination is visible in what portion of the LIA

A

Aerobic Slant portion

60
Q

Lysine decarboxylation is visible in what portion of the LIA

A

Anaerobic Butt Portion

61
Q

Enzyme producing indole

A

Tryptophanase

62
Q

Indole production is used to determine the ability of an organism to split tryptophan to form the compound Indole

A

SIM

Sulfide Indole Motility Test

63
Q

Positive result in Indole Test

A

Pink to Wine Colored Ring after the addition of Kovak’s reagent

64
Q

Kovak’s/Ehrlich Reagent

A

P-dimethylaminobenzaldehyde

65
Q

Use of filter paper strips impregnanted with p-diamonocinnamaldehyde

A

Rapid Spot Indole Test

66
Q

Positive result in Rapid spot Indole Test

A

Blue

67
Q

Mixed acid of glucose fermentation

A

Methyl Red Test

68
Q

Culture medium for MR Test

A

MRVP broth/Peptone Glucose Broth

69
Q

VP reagents

A

40% KOH

Alpha-naphthol

70
Q

Indole, Methyl Red, Vogues-Proskauer Test positive Result

A

Red color

71
Q

MR (+) result

A

Red at pH 4.4 or less

72
Q

All are MR (+) except

A

VP KESH

VP (+)

Klebsiella-Enterobacter
Serratia-Hafnia

73
Q

Bacteria that are positive with the MR test usually have a ____ reaction on the VP test

A

negative

74
Q

VP test that uses alpha naphthol and KOH

A

Barrits method

75
Q

VP test that uses alpha naphthol in 40% KOH in creatine

A

Coblentz Method

76
Q

Phenyl pyruvic acid in PAD test is detected by adding few drops of

A

10% Ferric Chloride

77
Q

pH indicator in utilization tests

A

Bromthymol Blue

78
Q

Citrate utilization test is used to determine the ability of an organism to utilize sodium citrate as its only _____ source and inorganic ammonium salts as its only _____ source

A

Carbon and Nitrogen

79
Q

+ result in utilization test

A

Blue

80
Q
  • result in utilization test
A

Green

81
Q

Positive and Negative Control in Citrate Test

A

(+) K. pneumoniae

(-) E. coli

82
Q

Positive and Negative Control in Acetate Test

A

(+) E. coli

(-) Shigella

83
Q

Positive and Negative Control in Acetamide Test

A

(+) P. aeruginosa

(-) S. maltophilia

84
Q

Positive and Negative Control in Malonate Test

A

(+) Citrobacter

(-) E. coli

85
Q

Malonate (+) Organisms

A

KECH

Klebsiella
Enterobacter
Citrobacter
Hafnia

86
Q

End product in utilization teats

A

Ammonia

87
Q

Urea hydrolysis is also known as

A

Christensen’s Method/Urease Method

88
Q

Urea hydrolysis end product

A

Ammonium Carbonate

89
Q

Rapid urease producers

A

Proteus and Morganella

90
Q

Urease (+) organisms

A

CY-KlePS

Citrobacter
Yersinia
Klebsiella
PPM 
Serratia
91
Q

Reagent in urease test

A

Urea disk dissolved in 1mL distilled h2o

92
Q

Positive result in urease test

A

Change in the color of slant from orange to magenta/pink

93
Q

Negative result in urea hydrolysis

A

Yellow medium

94
Q

Determines the ability of an organism to oxidatively deaminate phenylalanine to phenyl pyruvic acid

A

PAD test

Phenylalanina Deaminase Test

95
Q

KCN Broth Test (+) Result

A

Turbidity

96
Q

KCN broth test (+)

A

KCN
Klebsiella Citrobacter eNterobacter
And PPM, S. liquefacien

97
Q

Positive result in MUG Test

A

If fluorogenic - (+) Blue Fluorescence

If Colorimetric - (+) Yellow

98
Q

Gelatin Liquefaction/Hydrolysis Test reagent

A

Gelatin

99
Q

Gelatin Liquefaction/Hydrolysis Test (+) result

A

Liquefied gel

100
Q

Organisms positive with Gelatin Liquefaction/Hydrolysis Test

A

Serratia and Proteus

101
Q

Inhibitory agent in BGA

A

Brilliant Green Agar

= Brilliant Green

102
Q

pH indicator in BGA

A

Phenol Red

103
Q

BSA/Wilson Blair is for

A

S. typhi

104
Q

H2S indicator in TSI and BSA

A

Ferrous sulfate/ Ferrous ammonium sulfate (Aldave)

105
Q

H2S indicator in SSA

A

Ferric Citrate

106
Q

H2S indicator in XLD, HEA, LIA

A

Ferric ammonium citrate

107
Q

H2S indicator in SIM

A

Lead Acetate

108
Q

Confirmatory test for Enterobacteriaciae

A

Serotyping

109
Q

Lactose Fermenters possess these enzymes

A

B galactoside permease - facilitates the entry of the lactose molecule through the bacterial cell wall
B galactosidase - hydrolyze lactose into glucose and galactose

(absence of both: NLF)

Microbiology (9 decks)

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