Laboratory Tests for Enterobacteriaciae Flashcards
1
Q
Positive control in Oxidase Test
A
P. aeruginosa
2
Q
Negative control in Oxidase Test
A
E. coli
3
Q
Organisms that do not grow on Mac
A
Haemophilus Pasteurella
HACEK Legionella
Parvobacteria Moraxella
Francisella
Brucella
4
Q
Reagents in Nitrate Reduction Test
A
Sulfanilic Acid
Alpha-napthylamine
5
Q
Added in Nitrate Reduction Test to confirm negative reaction
A
Zinc dust/powder
6
Q
Positive result in nitrate reduction test
A
Red color
7
Q
Determines whether the organism is a slow or LLF or true NLF
A
ONPG test
8
Q
Positive result in ONPG test
A
Yellow color within 20 mins
9
Q
Decarboxylase Test is also known as
A
Moeller’s Method
10
Q
pH indicator in Decarboxylase Test
A
Bromcresol Purple
11
Q
decarboxylase
Lysine ——————–>
A
Cadaverine
12
Q
decarboxylase
Ornithine ——————–>
A
Putrescine
13
Q
dihydrolase
Arginine ——————–>
A
Citrulline
14
Q
Promotes anaerobiosis in decarboxylase test
A
Mineral Oil
15
Q
Positive result in LOA test
A
Alkaline product - purple
16
Q
Negative result in LOA test
A
Yellow color (fermentation of glucose)
17
Q
Amino acid enzyme that requires aerobic environment
A
Deaminase
18
Q
Decarboxylase Test is also known as
A
Moeller’s Method
19
Q
pH indicator in Decarboxylase Test
A
Bromcresol Purple
20
Q
decarboxylase
Lysine ——————–>
A
Cadaverine
21
Q
decarboxylase
Ornithine ——————–>
A
Putrescine
22
Q
dihydrolase
Arginine ——————–>
A
Citrulline
23
Q
Promotes anaerobiosis in decarboxylase test
A
Mineral Oil
24
Q
Positive result in LOA test
A
Alkaline product - purple
25
Negative result in LOA test
Yellow color (fermentation of glucose)
26
Amino acid enzyme that requires aerobic environment
Deaminase
27
Decarboxylase Test is also known as
Moeller's Method
28
pH indicator in Decarboxylase Test
Bromcresol Purple
29
decarboxylase
| Lysine -------------------->
Cadaverine
30
decarboxylase
| Ornithine -------------------->
Putrescine
31
dihydrolase
| Arginine -------------------->
Citrulline
32
Promotes anaerobiosis in decarboxylase test
Mineral Oil
33
Positive result in LOA test
Alkaline product - purple
34
Negative result in LOA test
Yellow color (fermentation of glucose)
35
Amino acid enzyme that requires aerobic environment
Deaminase
36
Decarboxylase Test is also known as
Moeller's Method
37
pH indicator in Decarboxylase Test
Bromcresol Purple
38
decarboxylase
| Lysine -------------------->
Cadaverine
39
decarboxylase
| Ornithine -------------------->
Putrescine
40
dihydrolase
| Arginine -------------------->
Citrulline
41
Promotes anaerobiosis in decarboxylase test
Mineral Oil
42
Positive result in LOA test
Alkaline product - purple
43
Negative result in LOA test
Yellow color (fermentation of glucose)
44
Amino acid enzyme that requires aerobic environment
Deaminase
45
Ratio of glucose, lactose, sucrose in TSI
1:10:10
46
pH indicator in TSI
Phenol Red
47
H2S indicators in TSI
Ferrous sulfate
| Sodium Thiosulfate
48
TSI reaction when 2-3 sugars are fermented
A/A = LF
49
TSI reaction when glucose is fermented
K/A =NLF
50
TSI reaction when no sugar is fermented
K/K (Non fermentative: P. aeruginosa)
51
Formation of bubbles or splitting of the media in the butt in TSI indicate
Gas production
52
Used to determine whether a Gram-negative rod decarboxylates or deaminates lysine and forms H2S
LIA
53
Components of LIA
Lysine, Peptones, 0.1% Glucose
54
H2S indicators in LIA test
Sodium Thiosulfate
| Ferric ammonium citrate
55
pH indicator in LIA test
Bromcresol purple
56
(-) Lysine Deamination
| (+) Lysine Decarboxylation
K/K
| Purple/Purple
57
(-) Lysine Deamination
| (-) Lysine Decarboxylation
K/A
| Purple/Yellow
58
(+) Lysine Deamination
| (-) Lysine Decarboxylation
R/A
| Red/Yellow
59
Lysine deamination is visible in what portion of the LIA
Aerobic Slant portion
60
Lysine decarboxylation is visible in what portion of the LIA
Anaerobic Butt Portion
61
Enzyme producing indole
Tryptophanase
62
Indole production is used to determine the ability of an organism to split tryptophan to form the compound Indole
SIM
| Sulfide Indole Motility Test
63
Positive result in Indole Test
Pink to Wine Colored Ring after the addition of Kovak's reagent
64
Kovak's/Ehrlich Reagent
P-dimethylaminobenzaldehyde
65
Use of filter paper strips impregnanted with p-diamonocinnamaldehyde
Rapid Spot Indole Test
66
Positive result in Rapid spot Indole Test
Blue
67
Mixed acid of glucose fermentation
Methyl Red Test
68
Culture medium for MR Test
MRVP broth/Peptone Glucose Broth
69
VP reagents
40% KOH
| Alpha-naphthol
70
Indole, Methyl Red, Vogues-Proskauer Test positive Result
Red color
71
MR (+) result
Red at pH 4.4 or less
72
All are MR (+) except
VP KESH
VP (+)
Klebsiella-Enterobacter
Serratia-Hafnia
73
Bacteria that are positive with the MR test usually have a ____ reaction on the VP test
negative
74
VP test that uses alpha naphthol and KOH
Barrits method
75
VP test that uses alpha naphthol in 40% KOH in creatine
Coblentz Method
76
Phenyl pyruvic acid in PAD test is detected by adding few drops of
10% Ferric Chloride
77
pH indicator in utilization tests
Bromthymol Blue
78
Citrate utilization test is used to determine the ability of an organism to utilize sodium citrate as its only _____ source and inorganic ammonium salts as its only _____ source
Carbon and Nitrogen
79
+ result in utilization test
Blue
80
- result in utilization test
Green
81
Positive and Negative Control in Citrate Test
(+) K. pneumoniae
| (-) E. coli
82
Positive and Negative Control in Acetate Test
(+) E. coli
| (-) Shigella
83
Positive and Negative Control in Acetamide Test
(+) P. aeruginosa
| (-) S. maltophilia
84
Positive and Negative Control in Malonate Test
(+) Citrobacter
| (-) E. coli
85
Malonate (+) Organisms
KECH
Klebsiella
Enterobacter
Citrobacter
Hafnia
86
End product in utilization teats
Ammonia
87
Urea hydrolysis is also known as
Christensen's Method/Urease Method
88
Urea hydrolysis end product
Ammonium Carbonate
89
Rapid urease producers
Proteus and Morganella
90
Urease (+) organisms
CY-KlePS
```
Citrobacter
Yersinia
Klebsiella
PPM
Serratia
```
91
Reagent in urease test
Urea disk dissolved in 1mL distilled h2o
92
Positive result in urease test
Change in the color of slant from orange to magenta/pink
93
Negative result in urea hydrolysis
Yellow medium
94
Determines the ability of an organism to oxidatively deaminate phenylalanine to phenyl pyruvic acid
PAD test
| Phenylalanina Deaminase Test
95
KCN Broth Test (+) Result
Turbidity
96
KCN broth test (+)
KCN
Klebsiella Citrobacter eNterobacter
And PPM, S. liquefacien
97
Positive result in MUG Test
If fluorogenic - (+) Blue Fluorescence
| If Colorimetric - (+) Yellow
98
Gelatin Liquefaction/Hydrolysis Test reagent
Gelatin
99
Gelatin Liquefaction/Hydrolysis Test (+) result
Liquefied gel
100
Organisms positive with Gelatin Liquefaction/Hydrolysis Test
Serratia and Proteus
101
Inhibitory agent in BGA
Brilliant Green Agar
| = Brilliant Green
102
pH indicator in BGA
Phenol Red
103
BSA/Wilson Blair is for
S. typhi
104
H2S indicator in TSI and BSA
Ferrous sulfate/ Ferrous ammonium sulfate (Aldave)
105
H2S indicator in SSA
Ferric Citrate
106
H2S indicator in XLD, HEA, LIA
Ferric ammonium citrate
107
H2S indicator in SIM
Lead Acetate
108
Confirmatory test for Enterobacteriaciae
Serotyping
109
Lactose Fermenters possess these enzymes
B galactoside permease - facilitates the entry of the lactose molecule through the bacterial cell wall
B galactosidase - hydrolyze lactose into glucose and galactose
(absence of both: NLF)
