Gram Negative Cocci Flashcards

1
Q

Anaerobic Gram Negative Cocci

A

Veionella

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2
Q

Old name of Neisseria

A

Streptococcus

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3
Q

Presumptive Test for Neisseria

A

Oxidase Test

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4
Q

(+) CAP

(-) BAP

A

N. gonorrheae

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5
Q

(+) CAP

(+) BAP

A

N. meningitides

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6
Q

Yellow-Pigmented Neisseria

A

N. subflava/N. flavescens

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7
Q

Obligate aerobe, capnophilic, non-motile, no -hemolytic and are carbohydrate fermenters

A

Neisseria

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8
Q

Neisseria gonorrheae requires ____ for growth.

A

Iron

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9
Q

Principal virulence factor of Neisseria

A

Common pili

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10
Q

Important in Neisseria’s pathogenesis in mucous membrane secretions

A

IgA Protease

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11
Q

Virulent colonial types of Neisseria

A

T1 and T2

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12
Q

Avirulent colonial types of Neisseria

A

T3 and T5

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13
Q

Means a “flow of seed” and “brothel”

Acute pyogenic infection of nonciliated columnar and transitional epithelium

A

Gonorrhea

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14
Q

3 causative agents of PID

A
  1. C. trachomatis
  2. N. gonorrheae
  3. M. hominis
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15
Q

CA of Fitz-Hugh-Curtis syndrome

A

Neisseria gonorrheae

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16
Q

CA of opthalmia neonatorum

A

N. gonorrheae

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17
Q

Transport medium for Neisseria

A

Amies with charcoal
Transgrow
Cary Blair
JEMBEC

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18
Q

Swabs for Neisseria should be plated within ____ hours

A

6 hours

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19
Q

True or false

Neisseria will not be recovered from routine blood cultures because it is inhibited by SPS

A

True

Inhibited by SPS: (Sm PaNGV)

S. monoliformis
P. anaerobius
Neisseria
G. vaginalis

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20
Q

Neisseria is a cold-sensitive organism. Thus, specimens should be kept at RT or placed ar 37 C before plating.

True or False

A

True

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21
Q

The appearance of intracellular Gram-negative diplococci, kidney or coffee-bean shaped is DIAGNOSTIC.

True or False

A

True

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22
Q

> 5 PMNs/field but no bacteria is indicative of

A

Non gonococcal urethritis (C. trachomatis)

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23
Q

JEMBEC stands for

A

John E. Martin Biological Environment Chamber

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24
Q

Antimicrobial agents in TMA

A

Vancomycin
Colistin
Nystatin

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25
Antimicrobial agents in MTM
All TMA components + Trimethoprim Lactate
26
Trimethoprim Lactate inhibits
Swarming of Proteus
27
Antimicrobial agents in Martin Lewis Medium (ML)
Vancomycin Colistin Anisomycin
28
Anisomycin in ML inhibits the growth of
Yeast
29
A transparent Gonoccocal medium
New York City Medium
30
Antimicrobial agents in NYC medium
Vancomycin Colistin Amphotericin B Trimethoprim (VCAT)
31
Antimicrobial agents in GC-LECT medium
NYC antibiotics + Lincomycin
32
Other bacteria that can grow in gonococcal media (3)
Si King nakaCAP sa Cine Kingella denitrificans Capnocytophaga Acinetobacter
33
Standard method of identifying N. gonorrhea
CHO utilization test
34
Medium used in CHO utilization test
Cystine Trypticase Agar (CTA)
35
CTA consists of
1% CHO + Phenol Red
36
+ result in CHO utilization test
Yellow color
37
Reagent in Oxidase Test
1% tetramethyl-p-phenylenediamine dihydrochloride
38
Positive result in oxidase test
Purple color within 10 seconds
39
Reagent in superoxol test/modified catalase test
20-30% H202
40
Positive result in superoxol test
Vigorous Bubbling
41
Causative agent of Clap
Clap = Gonorrhea N. gonorrheae
42
Taxo N
Cytochrome Oxidase | Indophenol Blue
43
Oxidase Test can be done in (3)
1. colony 2. filter paper 3. Disk
44
True or False CTA in CHO utilization/fermentation test should be placed in a Non-CO2 incubator
True
45
Choromogenic cephalosporinase test (+) result
Pink/Red
46
Iodometric test + result
Colorless
47
Acidimetric + result
Yellow
48
Most sensitive beta lactamase test
Nitrocetin Test
49
Positive result in beta lactamase test
Color change
50
Vancomycin inhibits
Gram +
51
Colistin inhibits
Gram -
52
Nystatin inhibits
Yeast
53
Amphotericin B inhibits
Yeast
54
CA of Meningococcemia
N. meningitidis
55
Serotypes of N. meningitidis
A, B, C, Y, and W-135
56
CA of DIC
N. meningitidis
57
CA of Waterhouse-Friderichsen Syndrome (hemorrhage in the adrenals)
N. meningitids
58
Gamma-glutamyl aminopeptidase test (+)
N. meningitidis
59
Rod-shaped gram negative cocci that is weakly + or - in catalase test
N. elongata
60
Rod-shaped gram negative cocci that is weakly PAD +
N. weaveri
61
Normal flora of the URT
M. catarrhalis
62
3rd cause of otitis media
Moraxella catarrhalis
63
"honey puck apperance" colonies
M. catarrhalis
64
Wagon wheel appearance colonies
Old colonies of M. catarrhalis
65
M. catarrhalis grows on BAP at ___ C and on nutrient agar at ____ C
22 | 35
66
Assacharolytic in CHO degradation test
M. catarrhalis
67
DNAse (+)
M. catarrhalis
68
Glucose fermenter
N. gonorrheae
69
Glucose and Maltose fermenter
N. meningitidis
70
ONPG (+) | Glucose, Maltose, and Lactose fermenter
N. lactamica
71
Wrinkled colony | Glucose, Maltose and Sucrose fermenter
N. secca
72
DNT (+) DNAse Nitrate Reduction Test Tributyrin Hydrolysis Test
M. catarrhalis
73
Old name of Glucose
Dextrose
74
Old name of Sucrose
Saccharose
75
Butyrate esterase test/Butyrate Disk/Tributyrin HOH test (+) result
Blue color
76
Positive in Butyrate esterase test/Butyrate Disk/Tributyrin HOH test
M. catarrhalis
77
Specimen used in Butyrate esterase test/Butyrate Disk/Tributyrin HOH test
Eye or eye culture
78
3 organisms causing Otitis Media
1. S. pneumoniae 2. H. influenzae 3. M. catarrhalis
79
B-galactosidase (+)
N. lactamica
80
Superoxol (+)
N. gonorrheae