Enterobactericae (Biochem Test)

Question 1

(EMB)
Inhibitor:
Carbon:
Indicator:
Lactose fermenter:
Non Lactose fermenter:

Answer 1

Eosin Y, Methylene blue
Lactose
Eosin Y, Methylene blue
Red/Purple
Colorless

Question 2

(MAC)
Inhibitor:
Carbon:
Indicator:
Lactose fermenter:
Non Lactose fermenter:

Answer 2

Crystal violet, bile salt
Lactose
Neutral red
Red/pink
Colorless

Question 3

(XLD)
Inhibitor:
Carbon:
Indicator:
Lactose fermenter:
Non Lactose fermenter:

Answer 3

Bile salt
Xylose, lactose, sucrose
Phenol red
Yellow
Red

Question 4

(HEA)
Inhibitor:
Carbon:
Indicator:
Lactose fermenter:
Non Lactose fermenter:

Answer 4

Bile salt
Salicin, lactose, sucrose
Bromthymol blue
Yellow
Green

Question 5

(DCA)
Inhibitor:
Carbon:
Indicator:
Lactose fermenter:
Non Lactose fermenter:

Answer 5

Bile salt
Lactose
Neutral red
Red/pink
Colorless

Question 6

(SSA)
Inhibitor:
Carbon:
Indicator:
Lactose fermenter:
Non Lactose fermenter:

Answer 6

Bile salt
Lactose
Neutral red
Red
Colorless:
*NLF with black center - Salmonella
* NLF without black center - Shigella

Question 7

H2S Producers: SPACED

Answer 7

Salmonella
Proteus
Providencia
Arizona
Citrobacter freundii
Edwardsiella
(+): Blackening of medium

Question 8

(BSA)
Inhibitor:
Carbon:
Indicator:
Lactose fermenter:

Answer 8

Brilliant green
Glucose
Bismuth sulfite
Black colonies (+): Salmonella typhii

Question 9

(TCBS)
Inhibitor:
Carbon:
Indicator:
Lactose fermenter:
Non Lactose fermenter:

Answer 9

Bile salt
Sucrose
Bromthymol blue
Yellow
Green

Question 10

(TSI)
Carbon:
pH Indicator:
H2s indicator:

Answer 10

Sucrose, Lactose, Glucose + Iron + Na thiosulfate
Phenol red (red to yellow)
FeSo4 (black; gas = splits medium)

Question 11

TSI reactions:
A/A

Answer 11

Yellow/Yellow
lactose, sucrose, glucose fermented

Question 12

TSI reaction
K/A

Answer 12

Red/Yellow
Glucose fermented

Question 13

TSI: (A/A) - “EKEE + ACC”

Answer 13

E. coli
Klebsiella
Enterobacter aerogenes
Enterobacter cloacae

Arizona
Citrobacter freundii
Citrobacter diversus

Question 14

TSI: (K/A) - “PPM + SSSY”

Answer 14

Proteus
Providencia
Morganella

All Salmonella except arizonae (LLF)
All Shigella except sonnei (LLF)
Serratia marcescens
Yersinia

Question 15

TSI: (K/K) - “PAA”

Answer 15

Pseudomonas
Alcaligenes
Acinetobacter

Question 16

Salmonella w/ small amount of H2S

Answer 16

Salmonella typhii

Question 17

Salmonella w/ large amount of H2S

Answer 17

Salmonella typhimurium

Question 18

TSI reaction K/K

Answer 18

Red/Red
Non sugar fermenter

Question 19

LIA test principle

Answer 19

Detect to ability of bacteria to degrade acid lysine

Question 20

Indicator of LIA

Answer 20

Bromcresol purple

Question 21

LIA butt is indicator for?

Answer 21

Lysine decarboxylation

Question 22

LIA slant is indicator for?

Answer 22

Lysine deamination

Question 23

Lysine decarboxylation (butt)
(+)/(-)

Answer 23

(+) purple
(-) yellow

Question 24

Lysine deamination (slant)
(+)/(-) result

Answer 24

(+) red
(-) purple

Question 25

IMViC (+) result of Indole

Answer 25

(+) red ring

Question 26

IMViC (+)/(-) result for Methyl red

Answer 26

(+) red - E. coli (+) yellow - Enterobacter cloacae

Question 27

IMViC (+)/(-) result for Voges Proskauer

Answer 27

(+) red - "KESH" (Klebsiella, Enterobacter, Serratia, Hafnia) (-) yellow

Question 28

Citrate utilization (+)/(-) result

Answer 28

(+) blue - Klebsiella pneumoniae (-) green - E. coli

Question 29

ACETATE/MALONATE/ACETAMIDE (AMA) (+) result

Answer 29

A - E. coli M - Salmonella A - Pseudomonas

Question 30

ACETATE/MALONATE/ACETAMIDE (AMA) (-) result

Answer 30

A - Shigella flexnerii M - Shigella A - Stenotrophomonas maltophilia

Question 31

Urease Test (+)/(-) result

Answer 31

(+) red (-) yellow

Question 32

Rapid Urease test - "PPM"

Answer 32

(+) after 2 hrs Proteus Providencia Morganella

Question 33

Slow Urease - "CKEYS"

Answer 33

(+) after 4 hrs Citrobacter Klebsiella Enterobacter gergovae Yersinia Serratia

Question 34

Lactose Fermentation (Rapid) - "EKE"

Answer 34

Has 2 enzymes Escherichia Klebsiella Enterobacter

Question 35

Enzymes on Rapid Lactose Fermenters

Answer 35

B galactosidase Lactose permease

Question 36

Enzyme on Late lactose fermenters

Answer 36

B galactosidase only

Question 37

Lactose fermentation (Late) - "S2 HYCS"

Answer 37

Salmonella arizonae Shigella sonnei Hafnia Citrobacter

Question 38

Lactose fermentation (Non) NLF

Answer 38

All Salmonella except arizonae All Shigella except sonne Yersinia Serratia Proteus Providencia Morganella Other enterobactericiae

Question 39

ONPG detects?

Answer 39

B galactosidase for LLF (+) ALL LATE LACTOSE FERMENTERS

Question 40

Decarboxylase uses 4 tubes: LOAC (+) result

Answer 40

Lysine Ornithine Arginine Control (+) Purple/Yellow/Purple

Question 41

PAD (Phenylalanine deaminase) (+)/(-) result

Answer 41

(+) green - "PPM" Providencia Proteus Morganella (-) E. coli