Streptococcus Flashcards by A A

Gram (+) in cocci and chains

Streptococcus

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Streptococcus motility

Non - motile

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Streptococcus is non sporeforming: (T/F)?

True

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Catalase (-), pinpoint colonies

Streptococcus

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Selective medium of streptococcus

PEA (Phenyl Ethyl Alcohol Agar)

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Classification based on antigens present on surface

Lancefield

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Classification based on hemolytic reaction

Smith & Brown’s

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Classification based on temperature

Bergey’s

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Lancefield group A

S. pyogenes

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Lancefield group B

S. agalactiae

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Lancefield group C

S. dysagalactiae
S. equisimilus
S. equisimilis
S. equi
S. zooepidimicus

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Lancefield group D

Enterococcus
E. faecalis
E. faecium
E. avium
E. ducans

Non enterococcus
S. bovis
S. equinus

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Streptococcus that causes colon cancer

S. bovis

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Alpha hemolytic

Streptococcus pneumoniae
Viridans
Group D

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Beta hemolysis

Lancefield ABCD

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Gamma hemolytic

Lancefield D

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Bergey’s grouping: pus producing streptococcus

Pyogenic

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Bergey’s grouping: used in making dairy products

Lactic

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Bergey’s grouping: normal flora of the gut

Enterococcus

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Bergey’s grouping: normal flora of upper respiratory tract

Viridans

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Gram (+) diplococci
Lancet shaped
Dome shaped colonies

S. pneumoniae

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Streptococcus catalase

(-)

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White pinpoint colonies

Streptococcus

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Virulence factor of (GA) S. pyogenes

M protein

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O2 labile, antigenic, anerobic, lytic

Streptolysin O

O2 stable, non antigenic

Streptolysin S

Causes Scarlet fever

S. pyogenes

Toxin that causes Scarlet fever

Erythrogenic toxin

Bacitracin/Taxo A of S. pyogenes

Sensitive

Flesh eating bacteria

S. pyogenes

Causes impetigo Pharyngitis Rheumatic heart dxs Rheumatic fever Acute glomerulonephritis (AGN) Erysipelas Necrotizing fasciitis

S. pyogenes

Flesh eating bacteria

S. pyogenes

Beta hemolytic Neonatal sepsis Normal flora of vagina and upper respiratory tract Pneumonia Bacteremia

S. agalactiae

#1 cause of neonatal meningitis Resistant to Taxo A, P and SXT

(GB) S. agalactiae

Group of Streptococcus that rarely cause disease

Group C & D

Causes UTI Pneumonia Cellulitis

Lancefield Group C

Group C: Bacitracin / SXT

(R)(S)

Test that differentiates Group D from other Strep groups

Bile Esculin Hydrolysis Test

Medium of Bile Esculin test

Bile Esculin Agar (BEA) contains 40% bile and esculin

Positive result for Bile Esculin

Blackening or brown ppt (+) Group D streptococcus

Enterococci: 6.5% NaCl / PYR

(+)(+)

Non enterococci: 6.5% NaCl / PYR

(-)(-)

Positive result of PYR

Red (+) Group D, Group A (S. pyogenes)

Meaning of CAMP test

Christine, Atkins, Munch, Peterson

Principle of CAMP test

CAMP factor enhances beta hemolysis

Positive result of CAMP test

Arrow head hemolysis (+) S. agalactiae (+) H. Pylori (+) Listeria monocytogenes - no arrow head but w/ hemolysis *Indicator: S. aureus

GA (S. pyogenes): Bacitracin / SXT

(S)(R) (+) PYRase

GB (S. agalactiae): Bacitracin / SXT

(R)(R) (+) CAMP (+) Sodium hippurate

Group C: Bacitracin / SXT

(R)(S)

GD (Enterococcus): Bacitracin/SXT

(R)(R) (+) BEA (+) 6.5 NaCl (+) PYRase

GD (Non enterococcus): Bacitracin/SXT

(R)(R) (+) BEA

Norma flora of orpharynx and nasopharynx

S. pneumoniae

Virulence factor of S. pneumoniae

Capsule

Alpha hemolytic No Lancefield group Causative agent of Lobar pneumonia

S. pneumoniae

#1 community acquired pneumonia in the Ph

Lobar pneumonia

#1 cause of adult bacterial meningitis Most common cause of otitis media

S. pneumonia

Normal flora of upper respiratory, G.I and Genitourinary tract

Viridans

Alpha hemolytic No Lancefield group Cause of subacute bacterial endocarditis

Viridans

Causes dental caries

Viridans

S. pneumoniae: Inulin fermentation / Bile solubility / Optochin (Taxo P) / Quellung

(+)(+)(S)(+)

Viridans: Inulin fermentation / Bile solubility / Optochin (Taxo P) / Quellung

(-)(-)(R)(-)

Detects pneumococcal antibody; skin test for S. pneumoniae

Francis test

Detects erythrogenic antigen; skin test for S. pyogenes

Dick's test (+) Redness

Skin test for Scarlet fever

Schultz-Charlton test (+) S. pyogenes - blanching of rash around injection site

Require cysteine for survival Shows satelliting on agar (+) Staph streak test

S. adjacens Abiotrophia spp.

Pediococcus: Vancomycin / Bile esc / PYR / NaCl / MRS / LAP

(R)(+)(-)(+)(-)(+)red

Leuconostoc: Vancomycin / Bile esc / PYR / NaCl / MRS / LAP

(R)(+)(-)(+)(+)gas(N/A)

Aerococcus: Vancomycin / Bile esc / PYR / NaCl / MRS / LAP

(S)(-/+)(-/+)(+)(N/A)(N/A)

Streptococcus Flashcards

(68 cards)