Enzyme Kinetics II

Question 1

in the concentration vs time reaction, when the reactions is starting what is high

Answer 1

substrate & enzyme

Question 2

in the concentration vs time reaction, when the reactions is starting what is low

Answer 2

ES low
product low

Question 3

the state when the enzyme is just enzyme with the substrate, it is called

Answer 3

pre-steady

Question 4

rate of formation of enzyme substrate complex is

Answer 4

directly proportional to the rate of the ES complex

Question 5

For an enzyme catalyzed reaction, if the Vo is 10mM min-1 when the substrate concentration is 10mM, then what must Vmax be if we know that Km=30mM

Answer 5

40 mM min-1

Question 6

what type of allostery is when it is the same ligand that is modifying the subunit to either positively or negatively

Answer 6

homotropic

Question 7

what type of allostery is when it is the different ligand that is modifying the subunit to either positively or negatively

Answer 7

heterophic

Question 8

when the binding of the inhibitor changes the site making it impossible for the substrate to bind is called

Answer 8

allosteric inhibition

Question 9

when the binding of the ligand changing the active site allowing for the substance to bind to the substrate is called

Answer 9

allosteric activation

Question 10

how are enzyme regulation

Answer 10

allostery
covalent modification
cleavage

Question 11

inactive form of enzyme is called

Answer 11

proenzyme=zymogen

Question 12

inactive form of chymotrypsin and trypsin

Answer 12

chymotrypsinogen and trypsinogen

Question 13

when pro is added to like protein or non enzyme, what doe that mean

Answer 13

procollagen

Question 14

what is the direction of converting inactive precursor to active enzyme by enzymatic cleavage

Answer 14

one-way street, unidirectional

Question 15

benefit of making an inactive enzyme to active enzyme

Answer 15

because u have to wait to get the stimulus from the cell to do that so the enzyme doesn’t eat up other rthings

Question 16

chymotrypsinogen chews to which amino acids

Answer 16

aromatic residue

Question 17

Trypsinogen chews at which amino acids residue

Answer 17

Lysine residue

Question 18

enzyme that add modification to the protein are called

Answer 18

writers

Question 19

enzyme that remove modification to the protein are called

Answer 19

erasers

Question 20

what do covalent structure affect

Answer 20

affect structure (therefore function)
changes charges
alter binding surface
blocks active sites

Question 21

what do covalent structure result in

Answer 21

enzyme on/off
change localization
change substrate preference
change binding partners
degradation

Question 22

—– enzyme adds phosphorylates and —– enzyme removes phosphorylation

Answer 22

kinases, phosphotases

Question 23

which is specific kinase or phosphotases

Answer 23

kinase

Question 24

glycogen phosphorylase exist in which form

Answer 24

active and less active

Question 25

methotrexate is used to

Answer 25

inhibit folic acid , reduces dntp

Question 26

round up used in our yards

Answer 26

has aromatic amino acid synthesis inhibitor

Question 27

molecules interfering with catalysis are called

Answer 27

enzyme inhibtors

Question 28

types os reversible inhibitors

Answer 28

competitive
uncompetitive
mixed
non competitive

Question 29

enzyme inhibitors can affect which values

Answer 29

Vmax & Km , Vmax apparent & K max apparent

Question 30

when an enzyme binds to its substrate and then an inhibitor looks exactly similar directly competing for the binding site, this is called

Answer 30

competitive inhibitor

Question 31

when an inhibitor binds to a completely different site on an enzyme that affects the way the enzyme function, this is called

Answer 31

uncompetitive inhibitor

Question 32

when an inhibitor changes the active site of the protein, this is called

Answer 32

noncompetitive inhibtor

Question 33

what changes in competitive inhibitor

Answer 33

no changes in Vmax, changes in Km

Question 34

in uncompetitive inhibitor, can the reaction move forward ?

Answer 34

no

Question 35

what change sin uncompetitive inhibitor

Answer 35

Vmax, Km

Question 36

when binding E and ES at the site is distinct from the substrate, this is called

Answer 36

mixed competitor

Question 37

which inhibitor binds like a substrate and is acted upon by enzyme at some point changes the structure of the enzyme

Answer 37

suicide inhibitor