Enzymes

Question 1

All enzymes are

Answer 1

catalysts

Question 2

Enzymes do what?

Answer 2

increase rate of reaction by lowering activation energy

Question 3

True or False enzymes are NOT consumed in reaction

Answer 3

True

Enzymes NOT consumed in reaction

Question 4

Molecule which enzymes act upon

Answer 4

substrate

Question 5

Enzymes act on

Answer 5

substrates

Question 6

Measure of affinity that an enzyme has towards the substrate

Answer 6

Km

Question 7

decrease in KM will increase AFFINITY

Answer 7

inverse relationship

Question 8

Max velocity of an enzyme

Answer 8

VMAX

Question 9

Km = of VMAX

Answer 9

Km = ½ of max

Question 10

3 factors affecting reaction velocity

Answer 10

1. substrate concentration
2. Temperature
3. pH

Question 11

Maximal velocity of an enzyme catalyzed reaction

Answer 11

VMAX

Question 12

Amount of substrate required to reach ½ of VMAX

Answer 12

Km

Question 13

Increase in KM

No Change in VMAX

^ -

Answer 13

Competitive Inhibitors

Question 14

No change in Km

Decrease in VMAX

-V

Answer 14

Noncompetitive Inhibitor

Question 15

Competitive inhibitors bind to

Answer 15

substrate binding site of an enzyme

Question 16

Competitive Inhibitors will

Answer 16

1. increase Km and lower affinity
2. no change max
3. add enough substrate to dilute out inhibitors

Question 17

Noncompetitive inhibitors will bind to

Answer 17

site other than substrate binding site

Question 18

Noncompetitive inhibitors will

Answer 18

1. no change in Km
   
   1. decrease VMAX will take longer to reach VMAX

Question 19

measure of amount of energy available to determine if reaction is feasible

Answer 19

Gibbs Free Energy

Question 20

Delta G = 0

Answer 20

equilibrium

Question 21

Delta G = +

Answer 21

endothermic or endergonic

nonspontaneous

unfavorable

Question 22

Delta G = -

Answer 22

exothermic exoergonic

spontaneous and favorable