ENZYMES (mostly) Flashcards
(17 cards)
What are the 4 main enzymes?
Carbohydrase
Lipase
Amylase
Protease
What does protease break down?
Protein into amino acids
What does carbohydrase break down?
Carbohydrates into sugars
What does lipase break down?
Fats into fatty acids and glycerol
What does amylase break down?
Starches into sugars
The 3 parts of the human digestive system that produce amylase?
Small intestine
Pancreas
Salivary glands
The 3 parts of the human digestive system that produce lipase?
Pancreas
Mouth
Stomach
The 3 parts of the human digestive system that produce carbohydrase?
Mouth
Pancreas
Small intestine
The 3 parts of the human digestive system that produce protease?
Stomach
Pancreas
Small intestine
Explain how amylase breaks down starch in terms of the lock and key theory?
Amylase breaks down starch into sugars.
Enzymes have a specific active site.
The shape is complimentary to the substrate.
What is denaturing in enzymes?
If enzymes are exposed to extremes of pH or high temperatures the shape of their active site may change.
What does the liver produce and the gall bladder store? What does it do?
Bile which can help neutralise stomach acid and emulsifies fats into small droplets.
What chemical is used to test for starch?
What’s the method? What colour does it start and end?
Iodine.
3 drops of iodine to the food.
It starts orange-brown and ends blue/black.
What chemical is used to test for protein?
What’s the method? What colour does it start and end?
Biuret Solution.
Add a few drops of copper sulfate and sodium hydroxide.
It starts blue and ends purple.
What chemical is used to test for glucose? (TRAFFIC LIGHT)
What’s the method? What colour does it start and end?
Benedict’s solution.
Add a few drops of Benedict’s solution and heat the test tube in a water bath at 60-70°.
It starts blue and ends red (lots of sugar), amber (medium) and green (small)
What chemical is used to test for fats?
What’s the method? What colour does it end with if it contains fats?
Filter paper or ethanol.
Rub some food onto filter paper and hold up to the light.
If it turns translucent, it contains fats.
What do enzymes do to chemical reactions?
Speed them up.