Enzymology 2

Question 1

Factor that effect enzymes activity

Answer 1

enzymes concentration
substrate concentration
temperature
pH level

Question 2

competitive inhibitor and non competitive inhibitor are under which inhibitor

Answer 2

reversible inhibitors

Question 3

Define competitive inhibition

Answer 3

Substrate bind at active site

half of the enzymes trapped by inhibition; half catalysis to form product

**increase in Km; Vmax maintain
**
Excess substrate will abolishes (废除) the inhibition

If concentration of substrate more than inhibitor, inhibition will reversed

Question 4

Define Non-competitive inhibition

Answer 4

inhibitor bind with enzymes at allosteric site, so no substrate can be bind to the enzymes

Km maintain; Vmax decrease

Excess substrate will abolishes (废除) the inhibition

If concentration of substrate more than inhibitor, inhibition will not relieve

Question 5

Allosteric enzyme feature

Answer 5

Allosteric enzymes has catalytic site where substrate binds and another separate allosteric site where inhibitor/ activator bind

Question 6

definition of allosteric regulation

Answer 6

An activator or inhibitor binds to enzymes other than the active site (allosteric site)

Question 7

Definition of feedback inhibition

Answer 7

Mechanism in which the accumulated end product binds with the enzyme and inhibits the enzymes activity

Question 8

Product binding of feedback inhibition

Answer 8

End product will binds with the active site of enzymes