exam 1 Flashcards
(87 cards)
1
Q
hematology
A
study of formed elements in blood
RBCs, WBCs, and platelets
2
Q
chemistry
A
testing concentration of chemical constituents of bodily fluids and blood & blood collected in plasma separator/serum separator tubes
3
Q
microbiology
A
planting specimens onto culture media
4
Q
histology
A
study of tissue structure
5
Q
example of accessioning
A
troubleshooting specimens and requisition errors
6
Q
transfusion medicine
A
requires 2 EDTA tubes & providing blood components to patients
7
Q
cytology
A
study of cells
8
Q
coagulation
A
assess bleeding and clotting problems
9
Q
example of hematology
A
- bone marrow analysis
- complete blood counts, ESR, white cell differential
10
Q
example of microbiology
A
- study of microorganisms
- identification of pathogenetic bacteria
11
Q
example of accessioning
A
- distribute specimens to the appropriate testing department
12
Q
example of transfusion medicine
A
- called immunohematology
- type and screen test
13
Q
example of cytology
A
- pap smear
- cancer
14
Q
example of coagulation
A
- activated partial thromboplastin time
- PT/INR
- FIB tests
15
Q
identifying critical results (pre, analytical or post)
A
post analytical
16
Q
loading specimens onto analyzers (pre, analytical or post)
A
analytical
17
Q
patient preparation for collection (pre, analytical, or post)
A
pre analytical
18
Q
quality control procedure (pre, analytical, or post)
A
analytical
19
Q
includes the majority of MLAs workload (pre, analytical or post)
A
pre analytical
20
Q
specimen transport (pre, analytical or post)
A
pre analytical
21
Q
disposal of old specimens and reagents (pre, analytical, or post)
A
post analytical
22
Q
maintenance of automated instruments (pre, analytical, or post)
A
analytical
23
Q
specimens collected properly (pre, analytical or post)
A
pre analytical
24
Q
reporting and archiving results (pre, analytical, or post)
A
post analytical
25
tolerance is the same as *** and means:
accuracy
means: nearness to true value, or limits of error
26
precision is the same as **** and means:
reproducibility
means: repeatability
27
glassware calibrated at *** degrees celsius
20
28
if a pipette is calibrated for both TC and TD, which marking will be the top one?
TD will be top mark
29
what are volumetric pipettes used for?
accurate measurement of aqueous solutions
30
why is the tip wiped before, not after, adjusting the meniscus to the calibration mark?
wiping draws some of the liquid out of pipette
31
how will bubbles in a pipette tip affect the volume delivered
less volume will be delivered
32
what should be done to an MLA pipette if the plunger doesn’t slide smoothly?
clean and lubricate the piston.
33
what should be done to an MLA pipette if it doesn’t pick up the correct amount of sample
replace the seals
34
what should be done to an MLA pipette if the nozzle is clogged
use a wire or blow air through
35
how many stops does an eppendorf have
2 stops
36
compare cylinders to volumetric flasks in terms of accuracy
volumetric flasks are more accurate
37
when would a TC cylinder be used
to make less accurate solutions, to measure urine volumes
38
when would a TD cylinder be used
to measure and deliver liquids into another container
39
how can you determine if all detergent has been rinsed off glassware
use an indicator — changes colour at alkaline pH (from soap)
40
the last rinsing must be done with **** water
distilled
41
what type of filter used in a BSC
HEPA filters
high efficiency particulate air
42
when does a safety cabinet require certification?
annually
when moved
when filter is replaced
43
what is centrifuge used for
used to accelerate gravitational separation of substances
44
what is the formula for calculating RCF
RCF = Krn^2
45
can bleach be used to disinfect centrifuges? why
no, it will corrode metal
46
what is the technique for using a serological pipette?
blow out the last drop at the tip
47
identification of specimen integrity is part of post analytical workflows? T of F
false
48
get to eyewash station within… and for how long
within 10 seconds for 15 mins
49
safety showers have to be how long
atleast 15 mins
50
biological spills
disinfectant soak for 30 mins
51
tolerance and accuracy
refer to limits if error/nearness to true value
52
precision and reproducibility
refer to ability to repeatedly produce the same value
53
TC
to contain
glassware has been calibrated to HOLD the stated volume
54
TD
to deliver
glassware is calibrated to DELIVER an accurate volume to another container
55
refrigerator temp normally?
4 degrees celsius
56
lab freezers usually at?
-70 degrees celsius and -30 degrees celsius
57
BSC is used to?
provide workspace that prevents exposure to infectious aerosols
58
k value is what
1.118x10-5
59
the time required to separate mixers of varying densities depends on:
rotor speed
radius of rotor
effective path length traveled by the particles (depth of liquid in tube)
60
less time is needed to centrifuge a specimen if:
speed (rpm) is increased
radius is increased
depth of liquid is decreased
61
vortex
used to mix solutions in a quick, whirling motion
62
rockers and mixers
used to gently mix many test tubes to provide homogenous samples
63
reference electrode
produce a constant potential
64
indicator electrode
contains glass membrane attracts hydrogen ions
65
what is the most common test in microbiology
culture and sensitivity testing
66
what department is often the largest and most automated area of the lab?
chemistry department
67
examining hematology and coagulation provides valuable information to help diagnose and manage…
anemias, leukemias, infection and many other disorders
68
immunohematology testing is done on?
pregnant women to detect potential blood incompatibilities with their fetus
69
what is the procedure that the patient has to do for pre-transfusion testing?
type and screen
70
common test for microbiology is
the culture and sensitivity (c&s)
71
what temp are most glassware calibrated at?
20 degrees celsius
72
volumetric pipettes are calibrated T*
TD
to deliver
73
is a volumetric pipette or a graduate pipette more accurate and are they TD or TC
volumetric is the most accurate and they are both calibrated to deliver (TD)
74
are mohr pipettes calibrated to the tip
no.
not calibrated to the tip, therefore this pipette is not drained past the last mark of stem
75
are serological pipettes calibrated to the tip?
yes
76
glassware washed cycle:
pre rinse
detergent wash
first, second, and third rinses
distilled or deionized water rinse
77
standard refrigerator temp is?
4 degrees
78
lab freezers are commonly used at
-70 and -20 degrees celsius
79
the most critical part of a BSC is the **** filter and what does the filter stand for
HEPA
high efficiency particulate air
80
fume hood are used in chemical laboratories to…
draw away dangerous fumes, gases or vapours.
81
how long should the fume hood be turned on for before use and left on for after
15 mins before and 15 mins after
82
RCF stands for
relative centrifugal force
83
K=
1.118x10-5
84
r=
radius in cm
85
n=
the speed of rotation of the rotor in rpm
86
RCF is calculated as…
RCF=Krn^2
87
vortex
used to mix solutions in a quick whirling motion
