EXAM 2 P1

Question 1

culture media

Answer 1

defined as medium vs. complex medium

Question 2

defined medium

Answer 2

aka minimal medium
made with purified chemicals
glucose and amino acids
we know the concentration

Question 3

complex medium

Answer 3

aka undefined medium
made with some nutrient source
yeast and soy beans
don’t know or care about the concentration

Question 4

defined growth medium

Answer 4

works well with species with high biosynthetic capacity

Question 5

what is biosynthetic capacity

Answer 5

making your own vitamins and nutrients

Question 6

complex growth medium

Answer 6

needed for species with a lot of nutrient requirements

pathogens

Question 7

selective media

Answer 7

prevent the growth of many things, while still allowing the growth of others

Question 8

differential media

Answer 8

allow more things to grow, giving colonies a different appearance with dyes

Question 9

liquid medium

Answer 9

rapid and constant mixing

simple recovery of cells for further studies

Question 10

solid medium

Answer 10

isolation of individual colonies and pure cultures

can observe phenotypes and count the number of cells

Question 11

bacterial cell division

Answer 11

done through binary fission
cells grow large and decide to divide, usually symmetric down the middle. binary fission can be observed with fluorescent microscopy

Question 12

cytokinesis

Answer 12

process of splitting the cytoplasm in 2

involves septum formation

Question 13

septum

Answer 13

structure built to come down in between 2 halves of the cell and pinch off the membrane

Question 14

divisome

Answer 14

creates the septum

made of proteins beginning with Fts

Question 15

Fts proteins

Answer 15

filamentation temperature sensitive

Question 16

FtsZ

Answer 16

homolog of tubulin found in eukaryotic cells that form a filament around the cell’s center

Question 17

FtsA and ZipA

Answer 17

linked to the cytoplasmic membrane and connect to FtsZ.

hydrolyzes GTP

Question 18

FtsI

Answer 18

extends outside the membrane and is directly involved in synthesizing peptidoglycan

Question 19

conditional mutant

Answer 19

alteration of a gene or protein that works under some conditions

Question 20

Min proteins

Answer 20

help find the cell center

comes from minicell formation

Question 21

Min E

Answer 21

goes to cell poles and has an affinity for the curved membrane.
when at one pole it drives Min C and D out and oscillates it before going to the next one

Question 22

Min C and D

Answer 22

inhibit divisome formation by creating FtsZ

Question 23

minicells

Answer 23

mutants that divide near one pole rather than the middle
do NOT have a chromosome
produces a functional metabolizing cell with NO DNA