Exam 3 key problems Flashcards Preview

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Flashcards in Exam 3 key problems Deck (35):
1

Who does the blood evidence found at the crime scene most likely belong to?

a) the victim

b) suspect 1

c) suspect 2

d) both the victim and suspect 1

e) both the victim and suspect 2

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a) the victim

2

Who does the hair evidence found at the crime scene most likely belong to?

a) the victim

b)suspect 1

c) suspect 2

d) both the victim and suspect 1

e) both the victim and suspect 2

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b) suspect 1

3

What does the forensic DNA analysis say most clearly about suspect 1?

a) She is innocent

b) she is guilty

c) She was at the scene at the time of the crime

d) someone matching her genetic fingerprint was at the crime scene

e) there is no direct DNA evidence that she was at the crime scene

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b) she is guilty

4

What does the forensic DNA analysis say most clearly about suspect 2?

a) he is innocent

b) he is guilty

c) He was at the scene at the time of the crime

d) someone matching his genetic fingerprint was at thw crime scene

e) there is no direct DNA evidence that he was at the crime scene

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a) he is innocent

and 

e) there is no direct DNA evidence that she was at the crime scene

5

The use or alteration of cells or biochemicals to provide a useful product descibes

a) recombinant DNA technology

b) transgenic technology

c) biotechnology

d) gene targeting

c) biotechnology

6

Which of the technologies listed below is a valuable method for mass-producing drugs and other useful proteins?

a) recombinant DNA technology

b) transgenic technology

c) biotechnology

d) gene targeting

a) recombinant DNA technology

7

Manufacturing recombinant DNA molecules involves cutting a gene from its normal location, inserting it into a circular piece of DNA from a bacterial cell, and then transferring the circle of DNA to cells of another species. Which of the tools below is used to cut the gene from its normal location

a) restriction enzyme

b)plasmid

c) bacteriophage

d) vector

a) restriction enzyme

8

Manufacturing recombinant DNA molecules involves cutting a gene from its normal location, inserting it into a circular piece of DNA from a bacterial cell, and then transfering the circle of DNA to cells of another species, Which of the below describe the circular piece of DNA from a bacterial cell?

a) restriction enzyme

b) plasmid

c) bacteriophage

d) vector

b) plasmid

9

Which gene transfer technique involves a tiny needle, which is used to inject DNA into a cell lacking that DNA sequence?

a) electroporation

b) liposome transfer

c) microinjection

d) particle bombardment

c) microinjection

10

The process of ______ involves the introduction of a gene into a cell where it exchanges places with its counterpart in the host cell.

a) transgenic technology

b) gene targeting

c) knockout technology

d) recombinant DNA technology

b) gene targeting

11

Genetic enginerring manipulates gene products at the level of the 

a) protein

b) amino acid

c) DNA

d) RNA

c) DNA

12

A cDNA version of a gene includes

a) codons for a mature mRNA

b) sequences corresponding to promoters

c) sequences corresponding to introns

a) codons for mature mRNA

13

________ are used to select genes of interest from a genomic library

a) Restriction enzymes

b) cloning vectors

c) DNA probes

d) gene targets

c) DNA probes

14

a) Draw the restriction map

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15

b) The mixture of fragments produced by the combined enzymes is cleaved with the enzyme EcoRI, resulting in the loss of the 3-kb fragment ( band stained with ethidium bromide on an agarose gel) and the appearance of a band stained with ethidium bromide representing a 1.5-kb fragment. Mark the EcoRI cleavage site on the restriction map

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16

a) Design the sense and antisense primer including the restriction enzyme need it to clone in the vector pREST A. Indicate the 5' position

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Sense: 5' BamHI or Kpn I or Eco RI - TGGCCGTGTG

Antisense: 5' Hind III - CCTAAAGTTCCT

17

b) Calculate the Tm of the oligonucleotieds

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Tm sense= 2(3) + 4(7) = 6 + 28 = 34C

Tm antisense= 2(7) + 4(5) = 14 + 20 = 34C

18

C) Indicate the PCR steps to amplify this gene including the temperature and the time for each one

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19

In our experiment, the normal hepatocyte sample is labeled with fluoresces red, and the sample with cncerous epatocytes were labeled with fluoresces blue. We apply the sample to the chip and then scan the chip. How many genes in chip depicted bellow are expressed only in cancer cell?

a) 15

b) 11

c) 8

d) 4

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b) 11

20

How many genes in chip depicted bellow is expressed in cancer and normal cells?

a) 15

b) 11

c) 8

d) 4

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d) 4

21

True or False: Sequence similarity searching algorithms like BLAST are based on the premise that if two sequences are similar then they are likely to be homologous

True

22

True or False: A motif is a distinctive pattern of amino acids, conserved across many proteins, which gives a particular fuction to the protein

True

23

Expression libraries are made with

a) Complementary DNA (cDNA)

b) Ribosomal RNA (rRNA)

c) messanger RNA (mRNA)

d) Double stranded DNA (dsDNA)

a) Complementary DNA (cDNA)

24

To understand the functions of genes we use reverse genetics and some of its tools are

a) RNAi

b) Knockout study

c) Microarray

d) a and b

e) a, b, and c

e) A, B, and C

25

Which of the following tools of recombinant DNA technology is INCORRECTLY paired with its use?

a) REstriction endonuclease - production of DNA fragments for gene cloning

b) DNA ligase - enzyme that cuts DNA, creating sticky ends

c) DNA polymerase - copies DNA sequences in the polymerase chain reaction

d) Reverse transcriptase - production of cDNA from mRNA

b) DNA ligase - enzyme that cuts DNA, creating sticky ends

26

The "Southern" technique involves:

a) The detection of RNA fragments on membranes by specific radioactive antibodies

b) The detection of DNA fragments on membranes by a radioactive DNA probe

c) The detection of proteins on membranes using a radioactive DNA probe

d) The detection of proteins on membranes using specific radioactive antibodies

e) The detection of DNA fragments on membranes by specific radioactive antibodies

b) The detection of DNA fragments on membranes by a radioactive DNA probe

27

"Gene library" is a term used to describe:

a) A computerized listing of known DNA sequences.

b) Bacteria with plasmids containing DNA fragments representing the majority of the genetic information from a plant or animal

c) A collection of books about recombinant DNA technology

d) Particle bombardment

b) Bacteria with plasmids containing DNA fragments representing the majority of the genetic information from a plant or animal

28

Extra info: A method for transferring protein from an electrophoresis gel to a permanent membrane or filter.

Western Blotting

29

Extra Info: A method for transferring mRNA from an electrophoresis gel to a permanent membrane or filter.

Northern Blotting

30

A multicellular organism that carries a specific genetic change in each cell because of an intervention at the fertilized egg stage is 

a) transversion

b) transition

c) transgenic

d) transformant

c) transgenic

31

The first drug produced using recombinant DNA tecnology is used to treat

a) hemophilia

b) dwarfism

c) heart attack

d) diabetes

d) diabetes

32

True or False: Restriction enzymes hydrolyze the phosphodiester backbone once on one strand

True

33

Which gene transfer technique involves the use of a fatty bubble to carry a gene into a somatic cell?

a) electroporation

b) liposome transfer

c) microinjection

b) liposome transfer

34

The " northern blot" technique involves:

a) the detection of RNA fragments on membranes by specific radioactive antibodies

b) the detection of DNA fragments on membranes by a radioactive DNA probe

c) the detection of proteins on membranes using a radioactive DNA probe

d) the detection of proteins on membranes using specific radioactive antibodies

e) the detection of DNA fragments on membranes by specific radioactive antibodies

a) the detection of RNA fragments on membranes by sepcific radioactive antibodies

35

True or False: Functional genomics seeks to understand the function of genes and how they determine phenotypes

True