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Flashcards in Old Exam 2 practice problems Deck (43):
1

The transfer of a naked fragment of DNA between bacteria is called?

Transformation

2

The transport of bacterial DNA to other bacteria via bacteriophages is called?

Transduction

3

What happens during a F+ xF- mating?

DNA is transferred from F+ to F- cells

4

Integrated (Hfr) - In this state the factor has integrated into the bacterial chromosome via a recombination. T or F?

True

5

The transfer of genetic information is in what direction?

Unidirectional and partial

6

What is a strain of bacteria that harbors a prophage is called?

Lysogen

7

T or F. In general transduction a lysogenic phage undergoes recombination with the host genome and later when an independent phage genome, and it carries one or more host genes with it.

False : It occurs in specialized transduction

8

An E. coli strain is F- lac Z- met+ bio+. Cells from this strain are mixed with an E. coli strain that is lac Z+ met- bio- and carrying an F' episome with the
plac O+ lac Z+ DNA sequence on the episome, and cultured for several hours. Then cells were removed, washed, and transferred to minimal media containing lactose as the only sugar source. A few cells were able to grow on minimal media with lactose, and formed colonies. How did these few cells become lac Z+ met+ bio+?

Sexduction

9

True and False. In the cut-and-paste transposition cointegrate intermediate is formed, both strands of the DNA transpose and transposon leaves the donor DNA.

False

10

________ is the recombination of DNA sequences having nearly the same nucleotide sequence and involving Rec A proteins

Homologous recombination

11

F factor plasmids play a major role in what bacterial process?

Conjugation

12

True or False. An Hfr ( high frequencey of recombination) strain occurs when the F+ factor becomes integrated into the E. coli host chromosome, due to the presence of the insertion sequences.

True

13

Usage of a virus vector to deliver the DNA into the target cell is what?

Transduction

14

This type of recombination does not require homologous sequences and is important for the integration of viral genomes into bacterial chromosomes are?

Site-specific recombination

15

Horizontal transfer can best be described as what?

The transmission of genetic information from one independent, mature organism to another.

16

The name of the process in which plasmids can be eliminated form a cells is called what?

Curing

17

The type of plasmid makes the host more pathogenic?

Virulence plasmid

18

What type of plasmid carries genes encoding enzymes that degrade substances such as aromatic compounds, pesticides, or sugar?

Metabolic Plasmid

19

Mobile genetic elements that carry the genes required for integration into host chromosomes are?

Transposons

20

Composite transposons are formed when?

Two IS elements integrate into a chromosome with only a small distance between separating them

21

In which type of transduction does the transducing particle carry only specific portions of the bacterial genome?

Specialized transduction

22

The tranposase gene encodes an enzyme that:

Facilitates site-specific integration of transposable elements

23

What are the three tools by microbial genetisists?

plasmids, bacteriophage, transposable elements

24

__________ promote the resolution of the cointegrates by recognizing the res sequences.

Resolvases

25

________ promote specific recombination by recognizing two sites that are in reverse orientation to each other.

DNA invertases

26

True or False: The ColE1-derived plasmid replication is regulated by a ctRNA and protein.

False

27

True or False: In the plasmids the oriV determine the mechanism of replication, host range, and regulate the number of copy.

True

28

True or False: The oriV region of ColE1-derived plasmid contains several repeats of a certain set of DNA bases (iteron sequences)

True

29

True or False: In R1 and ColB1-P9 plasmids the number of copies is regulated by the amount of Rep protein using antisense RNA to inhibit the synthesis of Rep protein.

True

30

An integrin with a large number of cassettes, what is it?

super-integron

31

What are mutations in the Lac Operon that seem to abolish expression called?

non-inducible

32

LacO mutations are cis dominant. True or False?

True

33

What are the three loci responsible for lactose metabolism?

LacZ, Lac Y, LacA

34

Bacteriophages need a host cell to reproduce. Name the two life cycles which phages can undergo and explain what the difference is for them.

Lytic and Lysogenic cycle

35

When a transposon moves within a host cell it?

Sometimes leaves a copy of itself from the original location

36

Type of site-specific recombinase that breaks and rejoins DNA in res sequences?

resolvase

37

What are the two classes of phage transduction

generalized and specialized transduction

38

In the absence of tryptophan, the trp repressor is?

Inactive and cannot bind to the operator

39

The regulation performed by regulatory genes; each step triggers the next and stops the preceding what?

Regulatory cascade

40

Single regulatory protein that controls a large number of operons.

Regulon

41

How many classes of CAP (catabolite activator protein) are there

Three; Class I, II, and III

42

The mRNA strand is coded by the what?

Anti-sense strand (3'-5')

43

How many classes of transposons are the there?

2; Class I and Class II