Exam 3 part 5 Flashcards Preview

Molecular Biology > Exam 3 part 5 > Flashcards

Flashcards in Exam 3 part 5 Deck (11):
1

attaches amino acids to tRNA

aminoacyl t-RNA synthetase

2

aminoacyl-tRNA synthetase has 2 active sites

synthesis site,
proofreading site

3

sites on ribosome

APE
Aminoacyl t-RNA binding site
Peptidyl-t-RNA binding site
Exit site

4

what delivers aminoacyl tRNAs?

EF-Tu
contains a GTP, which is hydrolyzed to make a GDP

5

moves tRNA from A to P site

EF-G

6

two ways of high fidelity

kinetic proofreading- ribosome waits to allow wrong tRNA to come off
induced fit- small ribosome holds mRNA, conformational change occurs when correct one is added

7

RNA molecules that posses catalytic activity

ribozymes

8

added for stop codon

release factor

9

sequence that marks the start codon in prokaryotes

Shine-Dalgarno sequence
AGGAGGU

10

sequence that marks the start codon in eukaryotes

Kozak sequence
ACC-AUG-G

11

eukaryotic initiation

eIF2 delivers the initiation meth to P site of small ribosome
eIF4E and eIF4G are cap binding proteins
when AUG is found, eIF2 is hydrolyzed
large ribosome joins small ribosome