Exam 4 part 3 Flashcards Preview

Molecular Biology > Exam 4 part 3 > Flashcards

Flashcards in Exam 4 part 3 Deck (18):
1

morphological changes in apoptotic cells

1. cell shrinks
2. cytoskeleton collapses
3. nuclear envelope disassembles
4. Chromatin condenses, then break into pieces
5. cell surface forms enclosed apoptotic bodies
6. apoptotic bodies absorbed by cells or macrophages

2

apoptosis

programmed cell death

3

necrosis

accidental cell death
cell swells and bursts spilling contents

4

TUNEL assay

Txt-mediated dUTP Nick End Labeling

5

Detection of Annexing V

1. changes in membrane during apoptosis\
2. phospholipid phophatidylserine flips to outer surface
3. Annexin V binds to phosphatidylserine

6

Annexin V purposes

1. Marker
2. "eat me" signal to macrophages
3. blocks inflammation

7

two major pathways of apoptosis

intrinsic pathway- trigger from inside cell
extrinsic pathway- triggered from outside by ligand

8

caspases

1. intracellular enzymes that trigger cell death
2. cysteine at active site
3. cleave aspartic acids

9

inactive precursor to caspase

procaspase

10

first caspase activated

initiator caspase
activates other procaspases
these new ones are executioner caspases

11

activate the extrinsic pathway of apoptosis

cell-surface death receptors
these receptors are from the TNF family

12

FADD

Fas-associated death domain
(on killer lymphocyte)

13

DISC

Death-inducing signaling complex

14

Cytochrome C

1. component of mitochondria ETC
2. binds to procaspase activating protein Apaf1
3. Apaf1 form a wheel heptameter called apoptosome
4. activates caspase-9, which activates executioner caspases

15

anti-apoptotic Bcl2 family protein

when active, keeps Bcl2 family proteins apart so they can't form a pore in the mitochondria

16

pro-apoptotic BH3-only protein

when active, deactivates anti-apoptotic Bcl2 family protein

17

block the caspases

IAPs

18

block IAPs

anti-IAPs released from mitochondria with the cytochrome C