Exam 6 Flashcards

(93 cards)

1
Q

What is the primary habitat for vibrio?
aeromonas?
C. violaceum

A

vibrio-brackish, salt water
Aeromonas- freshwater
C. violaceum- soil or water

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2
Q

What biochemicals for aeromonas

A

oxidase +
glucose fermenter
motile
indole +

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3
Q

Biochemicals for A. hydrophila

A

Arg +
orn -
TCBS-

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4
Q

What diseases do Aeromonas hydrophila cause

A

gastrointestinal
cellulitis
septicemia, meningitis,

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5
Q

What are Aeromonas species usually resistant to and S?

A

penicillin, ampicillin, carbenicillin cefazolin

S to 3rd gen cephalosporins, SXT, aminoglycosides

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6
Q

Oxidase +, GN, glucose fermenting

curved, motile, purple pigment

A

Chromobacterium violaceum

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7
Q

What biochemical test does not help ID chromobacterium and why

A

oxidase, because it is pigmented

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8
Q

Organism in soil and water
immunocompromised with low WBCs

A

C. violaceum

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9
Q

Almond Smell

A

C. violaceum

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10
Q

What biochemicals distinguish chromobacterium from other organisms

A

if pigmented, enough to ID it
if non pigmented, +indole, - Lys and orni
non maltose or mannitol fermentation

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11
Q

What is chromobacterium violaceum often resistant to

A

beta lactams and colistin

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12
Q

What do vibrio need for growth

A

need sodium, salt lovers

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13
Q

What do all vibrio species have in common in biochemicals

A

glucose fermenters mostly
all motile, catalase -, oxidase +
nitrate +

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14
Q

What temperature do Vibrio grow in

A

can be in water if more than 20C

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15
Q

Best way to differentiate Vibrio from aeromonas

A

String test
DNA
vibrio +
Aeromonas -

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16
Q

halophilic

A

vibrio

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17
Q

What special agars are used to culture Vibrio

How to interpret

A

stool- TCBS
yellow sucrose +

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18
Q

What does vibrio look like on MAC

A

all NLF except V.vulnificus

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19
Q

What agar is not very helpful for Vibrio

A

HE and XLD, shows as yellow, non selective to Vibrio

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20
Q

What special agar is used to find vibrio parahaemolyticus from seafood and what does it look like

A

chromogenic vibrio agar
mauve/ violet

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21
Q

What distinguishes vibrio from enterbacteriales in a stool culture
what species is it probably

A

oxidase +

probably cholera

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22
Q

How does Vibrio cholera spread and what does it cause

A

contaminated water or seafood, rapid flux and electrolyte loss
cholera toxin

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23
Q

Rice water stool

A

vibrio cholerae

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24
Q

What does Vibrio vulnificus cause and how is it transmitted

A

*** Fatal septicemia and especially + liver disease

can become cellulitis and necrotizing fasciitis, multiple organ failure

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25
What does vibrio parahaemolyticus cause and how is it acquired
raw seafood, watery diarrhea self limiting 24-48 hrs
26
What environment almost always has V. parahaemolyticus
in coastal environments
27
What does V alginolyticus cause
least pathogenic, eye and ear infection, wound and burn
28
Vibrio species that is oxidase -
Vibrio metschnikovii
29
Only vibrio species that is lactose fermenting/ MAC pink
Vibrio vulnificus
30
Which Vibrio species nearly always comes from extraintestinal sources
Vibrio algunolyticus
31
How are Vibrio species distinguished in the lab
sucrose fermentation TCBS is selective for all Vibrios
32
What Vibrio species is Bright Yellow on TCBS and black?
yellow cholerae black parahaemolyticus
33
What subgroups are withing V. cholera
antigen groups worst- O1- cholera toxin-fatal O139- makes toxin also others non O1/ non O139- no toxin
34
All NLF GNR are oxidase positive except
S maltophilia Acinetobacter V. metschnikovii
35
All GNR that are NLF are motile except
Burkholderia mallei
35
TSI restults for Burkholderia and Pseudomonas
gas + bubbles H2S + black butt - yellow underneath red slant
36
O/F results for Pseudomonas aeruginosa
Glucose Open tube - yellow- pos-oxidizer closed tube- green- neg fermenter
37
Best way to distinguish pseudomonas and burkholderia
pseudomonas is catalase + burkholderia catalase -
38
What organisms are BCSA agar for how to read
Burkholderia if translucent colony, red-orange agar then B cepacia
39
non wrinkled, rough, serrated edges on MAC
B. cepacia
40
What is the best way to ID pseudomonas and Burkholderia vs enterobacteriales
API 20NE
41
What do E.coli, Psuedomonas and Staph look like on MAC
E. coli- pink growth Pseudomonas- yellow growth Staph- no growth
42
What do B mallei and B. pseudomallei look like on a gram stain
mallei- coccobacillus on GNR pseudomallei - GNR small bipolar staining
43
Organism in eye drops and spas
Burkholdeereia cepacia
44
What major diseases do B. cepacia cause
cystic fibrosis pts
45
Organism that grows on onions
Burkholderia cepacia
46
What biochemicals Burkholderia cepacia Looo
oxidase slow weak + lysine + ornithine + ONPG +
47
What is Burkholderia cepacia resistant to Pac Best treatment?
aminoglycosides, polymyxin B, colistin lot of R, no good antibiotic treatment
48
Oxidizes lots of sugars glucose, lactose, maltose, mannitol
B. cepacia
49
Organism that is NLF, GNR, decarboxylates lysine in cerebral spinal fluid is probably
B. cepacia
50
What immunocompromised patients are at most risk of B cepacia infection
CGD chronic granuloma disease,
51
How does B cepacia affect immunocompetent people
infection of blood, UT, and respiratory tract rarely fatal
52
Rice paddies
B. pseudomallei
53
What organism is Ashdown medium for
B pseudomallei appears violet purple color
54
What do B. pseudomallei look like on a gram stain and BA?
gram- bipolar staining, safety pins wrinkled metallic varied hemolysis
55
organism that has an earthy odor
B. pseudomallei
56
Biochemicals for Burkholderia pseudomallei
oxidase + Arg + lactose oxidizer cetrimide neg
57
How to distinguish B. pseudomallei from pseudomonas?
B. pseudo cetrimide - Pseudomonas cetrimide +
58
What makes B pseudomallei look different from P. aeroginosa on a plate
no pyrverdin pigment on pseudo
59
What disease does B. pseudomallei cause
meliodosis- pulmonary disease very fatal
60
Causes disease amoung Vietnam vets and SE Asian immigrants
B. pseudomallei
61
Organism that causes infections in horses called Glanders
B. mallei
62
Organism that is a potential bioterrorist agent
B. mallei
63
strictly aerobic motile polar flagella with tufts
Pseudomonas
64
Organism that smells like grapes
P. aeroginosa
65
What diseases are P. aeruginosa associated with
Cystic fibrosis
66
What virulence factors do P. aeruginosa have***** practice matching
exotoxin A Exoenzymes S and T phospholipase C pilli alginate pyocyanin
67
Organism involved in quorum sensing What do they produce
P. aeroginosa allow it to produce a biofilm with alginate
68
swimmers or divers ear infection
P. aeruginosa
69
Hot tubs
P. aeroginosa
70
artificial nail infection
P aeruginosa
71
What organism is the most related to cystic fibrosis
P. aeroginosa
72
What does P aeruginosa look like on BAP and MAC
blue- pyocyanin green- pyoverdin flat, shiny, spready, metallic Beta
73
What is the only Pseudomonas species that grows at 42C
P. aeruginosa
74
Biochemicals for P. aeroginosa
Arg + citrate + gelatinase + cetrimide +
75
What is the only Pseudomonas species that produces Pyocyanin and what color is it
green-blue aeroginosa
76
What diseases do P. fluorescens and P. putida cause
rarely anything UTI, post-surgical abcesses septic arthritis
77
What Pseudomonas species produce pyoverdin only
fluorescens and putida
78
What are P. fluorescens and putida resistant to
carbenicillin and ticarcillin
79
What test is used to distinguish P. fluorescens from P putida
gelatin hydrolysis fluorescens + putida -
80
What does P. stutzeri look like on BA
tree root, wrinkled, leathery, bright yellow/ brown
81
What biochemical test distinguishes P. stutzeri
Arg neg
82
What environments are P orzyhabitans and luteola from
water and soil
83
sticky eye
P. oryzihabitans
84
What do orzyhabitans and luteola colonies look like
wrinkly yellow
85
Distinguishing biochemicals for P. luteola and oryzihabitans
oxidase -
86
What antimicrobial therapy is used to treat pseudomonas infections
beta lactam and aminoglycoside
87
How are Pseudomonas, Stenotrophomonas and Burkholderia distinguished
antimicrobial testing
88
Only organism mentioned to be non motile
B. mallei
89
On TCBS agar, why are organisms yellow
ability to oxidize sucrose
90
What is the most commonly encountered GNR genera outside of enterobacterales
Pseudomonas
91
What are the 3 organisms that are associated with cystic fibrosis, which is the most associated
Most- P. aeruginosa B. cepacia S. maltophilia
92
Vibrio body sites Cholerae alginolyticus vulnificus
cholerae-stool alginolyticus- skin, wound, ear vulnificus- blood