Exam 6 Flashcards
(93 cards)
What is the primary habitat for vibrio?
aeromonas?
C. violaceum
vibrio-brackish, salt water
Aeromonas- freshwater
C. violaceum- soil or water
What biochemicals for aeromonas
oxidase +
glucose fermenter
motile
indole +
Biochemicals for A. hydrophila
Arg +
orn -
TCBS-
What diseases do Aeromonas hydrophila cause
gastrointestinal
cellulitis
septicemia, meningitis,
What are Aeromonas species usually resistant to and S?
penicillin, ampicillin, carbenicillin cefazolin
S to 3rd gen cephalosporins, SXT, aminoglycosides
Oxidase +, GN, glucose fermenting
curved, motile, purple pigment
Chromobacterium violaceum
What biochemical test does not help ID chromobacterium and why
oxidase, because it is pigmented
Organism in soil and water
immunocompromised with low WBCs
C. violaceum
Almond Smell
C. violaceum
What biochemicals distinguish chromobacterium from other organisms
if pigmented, enough to ID it
if non pigmented, +indole, - Lys and orni
non maltose or mannitol fermentation
What is chromobacterium violaceum often resistant to
beta lactams and colistin
What do vibrio need for growth
need sodium, salt lovers
What do all vibrio species have in common in biochemicals
glucose fermenters mostly
all motile, catalase -, oxidase +
nitrate +
What temperature do Vibrio grow in
can be in water if more than 20C
Best way to differentiate Vibrio from aeromonas
String test
DNA
vibrio +
Aeromonas -
halophilic
vibrio
What special agars are used to culture Vibrio
How to interpret
stool- TCBS
yellow sucrose +
What does vibrio look like on MAC
all NLF except V.vulnificus
What agar is not very helpful for Vibrio
HE and XLD, shows as yellow, non selective to Vibrio
What special agar is used to find vibrio parahaemolyticus from seafood and what does it look like
chromogenic vibrio agar
mauve/ violet
What distinguishes vibrio from enterbacteriales in a stool culture
what species is it probably
oxidase +
probably cholera
How does Vibrio cholera spread and what does it cause
contaminated water or seafood, rapid flux and electrolyte loss
cholera toxin
Rice water stool
vibrio cholerae
What does Vibrio vulnificus cause and how is it transmitted
*** Fatal septicemia and especially + liver disease
can become cellulitis and necrotizing fasciitis, multiple organ failure