Exam 8 Flashcards by Sara Buitrago

What is the gram stain morphology of pasteurella
What biochemicals is it positive for

small GNR,
non motile
glucose fermenting
oxidase positive

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What media does pasteurella grow on

Chocolate and BAP
not MAC

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What is the epidemiology for pasteurella

zoonotic
normal flora in domestic and wild animals

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What bacteria is associated with bite wounds from dogs or cats

Pasteurella

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What is the most relevant species in pasteurella

P. multicoda

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What are the results for Pasteurella
oxidase
nitrate
ornithine
indole
urease

oxidase pos
nitrate pos
orni pos
indole neg
urease neg

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What is the plate morphology of pasteurella

gamma/ non hemolytic
musty smell
mucoid
no growth on MAC

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What antibiotics are useful for pasteurella multocida

penicillin

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How can you distinguish Pasteurella and Hemophilus

haemophilus do not grow on BAP

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What is the gram stain morphology for Bartonella

short Gram neg pleomorphic bacillus

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What agar does Bartonella grow on

BAP and CHOC not MAC

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What are the results for Bartonella
oxidase
catalase

oxidase neg
catalase neg

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Organism that can be acquired naturally or through anthroponotic/ human reservior

Bartonella

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Match the species to pathogenesis
B. quintana
B. bacilliformis
B. Henselae

Cat scratch fever
Oroya fever
Trench fever

B. quintana- trench fever
B. bacilliformis- Oroya fever
B. henselae- cat scratch fever

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What organism is capable of causing angiogenesis and define it.

Bartonella species, mimics cancer in the formation of new blood vessels

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What disease profile is this: progressive febrile anemia, verrugas on body

Oroya fever

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What disease profile is this
S&S like mild influenza, maculopapular rash, bone pain

Trench fever

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What special plates do Bartonella grow on

horse or rabbit blood agar

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Organism detected with 16S RNA, or genes gltA, groEL, ribC, ftsZ

Bartonella

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Match vector to organism: B. henselae, B, quintana
cat flea
human louse

B.henselae cat flea
B. quintana human louse

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What is the gram stain morphology of helicobacter

curved, helical or SPIRAL, fusiform pointed, GNR,

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What biochemicals is helicobacter positive for

urease strong pos
oxidase pos
catalase pos

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What organism is strongly related to stomach cancer

helicobacter

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How is helicobacter transmitted ?

fecal-oral and environmental

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How does helicobacter attack the stomach

escapes the acidity of the stomach, burrows it and colonizes the gastric mucosa, it neutralizes the stomach pH

What organism infects patients and develops peptic ulcer disease and gastric carcinoma

H. pylori

What organism produces a protein ____ that enables it to invade the gastric epithelium

H. pylori CagA

T or F H. pylori can be cultured with fecal specimens

false, they can only be used for antigen tests

How can H. pylori be detected with nucleic acid detection

gastric acid can be tested

How can you culture H. pylori

CHOC agar must be fresh and moist, needs high humidity

What organism can be IDed with a urea breath test

H. pylori

How can you distinguish H.pylori from vibrio and other gastrointestinal organisms

H. pylori does not cause diarrhea

What genes are used to ID helicobacter in PCR

ureA and cagA

What is used to detect helicobacter directly

with a special spectrometer

How is helicobacter detected in serology testing

J chain on IgA in stomach acids

How to distinguish Helicobacter and Campy

helicobacter- stomach and duodenum campy-small and large intestine

What is the most common cause of diarrhea in the US and worldwide due to its low ID___

campy id50

How is campy transmitted

by eating raw or **undercooked poultry **and seafood.

What kind of growth requirements do Campy have

slow growing, fastidious, microaerophillic, needs 5-10% O2

What are the 3 main diseases that campy casues

febrile systemic disease periodontal disease gastroenteritis

What organism has an S shape or seagull wing

Campy

What organism has darting motility

Campy

What are the reactions for Campy oxidase fermentation

oxidase pos non fermentation

What Campy species is associated with sepsis in the immunocompromised/ elderly

C. fetus

Organism is transmitted via contaminated food, milk or water and improperly pasteurized milk

campy

What organism on rare occasion causes Guillain Barre syndrome

Campy

T or F fecal white cells is a good test for finding Campy infections

False, better to take screening tests for inflammation, gram stains on stool is not recommended

What organisms can be detected in EIAs

Campy

How soon should a campy specimen be processed into the lab

2 hrs

What special agars are used to culture Campy

enriched BA CCDA

What is in CCDA

instead of blood it has charcoal, sodium pyruvate and ferrous sulfate

What kind of samples must be used to culture Campy species

must be from watery cultures, never formed stool

How much time does it take Campy to grow

less than 5 days in routine blood culture media

What is the colony morphology of Campy

gray-pink or yellow grey mucoid

How can you distinguish C. jejuni from C. coli

C. jejuni- sodium hippurate hydrolysis + C. coli neg

What campy species is incapable of growth in 42C, instead grows best at 37C-25C

C. fetus

What campy is not enteric

C. fetus

What are the results for NA and cephasporin disks for campy species jejuni, coli, lari and fetus

jejuni and coli are sensitive to NA and Resistant to Cephalothin fetu is R to NA and Sensitive to cephalothin C. lari is R to both

What antibiotics to treat Campy severe gastroenteritis infection

erythromycin

T or F, there are vaccines available for Campy

False, no vaccines

All legionella are

fastidious, motile, mesophilic, thin GNR

What organisms require irone, L cysteine, branched fatty acids and ubiquinones

Legionella

Organisms that are found in aquatic habitats at warmer temperatures, free flowing water, lakes, rivers

Legionella

Organism that uses biofilms to survive phagocytosis of monocytes and aveolar macrophages

legionella pneumophila

What is the main form of transmission of Legionella

inhalation of aerosols

What body sites can Legionella come from

respiratory secretions, sputum, bronchial lavage, lung biopsy

What disease profile is this febrile, cough, headache, pulmonary infiltrates

Legionnaire's disease

What media is used to culture legionella

BCYE

What organism causes pontiac fever

legionella

Organism that forms gray white colonies like ground glass on BCYE

Legionella

How can you distinguish L. pneumophila from other Legionella species

L. pneumophila does not fluoresce under UV light

What is the colony morphology of Legionella

Gram negative coccobacili or short rods, weakly staining, cut glass internal granular speckling

What are the results for Legionella oxidase catalase Beta lactamase

positive for all

How can you detect Legionella with Serology testing

serum species tested no closer than 2 weeks apart, after the appearance of the disease, antibody testing

What type of assay can be used to detect legionella

DFA direct fluorescent assay FITC conjugates

What DNA sequences for Legionella are tested

mip and wzm genes

What antibiotics should be used against Legionella

quinolones, tetracycline and macrolides

What growth requirements do HACEK organisms have

5-10% CO2, will not grow on MAC

All HACEK organisms

are dysgonic-grow slowly, ferment glucose, are fastidious, are GNR, grow on SBA and CHOC

What organisms need a CO2 incubator and candle jar

All HACEK organisms

HACEK organisms are normal flora of

nasopharynx and oral cavity of humans and other animals

HACEK that causes bacterial sub acute endocarditis, soft tissue infections

A, C and K aggregatibacter Cardiobacterium Kingella

HACEK that causes sepsis and endogenous infections of patients with neutropenia

Capnocytophaga

HACEK that causes DIC, renal failure, shock and HUS from animal bites

C. canimorsus and C. cynodegmi

What HACEK organisms cause endocarditis from peridontal disease

cardiobacterium

What HACEK organism causes osteoarthritis in children

Kingella

How to distinguish Actinobacillus and Aggregatibacter and Pasteurella

MALDI-TOF

What organism looks like dots and dashes of morse code

Actinobacillus

What organisms have a 4 to 6 pointed star in colonies

Actinobacillus actinomyecetemcomitans

Organism in HACEK that causes SBE subacute bacterial endocarditis

A. a horrible long name

What organism looks like tiny puffballs growing on blood cell layer in SBA

Aggregatibacter species

What organism can be found in dental plaque and gingival scrapings

Aggregatibacter aphrophilus

How can you distinguish Aggregatobacter aphrophilus from A. actino-horrible long name

actino- catalase pos lactose and indole neg Aggre- catalase neg lactose and indole pos

Organism that pits the agar but has no bleach odor

Carbiobacterium

Organism that has a gram stain with a teardrop appearance in clusters or rosettes

Carbiobacterium hominis

What are the results for cardiobacterium indole oxidase catalse

indole oxi pos catalase neg

Organism highly recovered in children's upper respiratory tract

Kingella

organism that looks like plump coccobacilli with squared off ends

Kingella species

What media is used to isolate Kingella species

MTM

How can you differentiate Kingella and Neisseria

Neisseria catalase neg Kingella catalase pos

What organisms affect bones and joints in children

Kingella kingae

HACEK that has gliding motility and is associated with dog bites

Capnocytophaga

What organism is inhibited by SPS

Capnocytophagia

What is the only Capnocytophaga species that is both catalase and oxidase pos

C. canimorsus

yellow-pigmented, thin, gram-negative rod with tapered ends that exhibits gliding motility and does not grow in ambient air

Capnocytophaga

What organism is encapsulates, plemorphic GNR

Klebsiella granulomatis

Organism that is inside macrophages or donovan bodies, cause donovanosis

Klebsiella granulomatis

Organism that can cause genital ulcers and subcutaneous nodules that enlarge

Klebsiella

Exam 8 Flashcards

(108 cards)