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Medical Microbiology > EXAM A > Flashcards

EXAM A Flashcards

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1
Q

Enterococcus has ________________ resistance

A

vancomycin

(VRE)

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2
Q

What differentiates S. pneumoniae from viridans group?

A

-Optochin sensitive/resistance was NOT an answer choice. That is usually the differentiating factor, but it wasn’t a choice.
-Viridans group and S. pneumoniae = alpha hemolytic

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3
Q

Bacitracin:

is Micrococci susceptible or resistant?

A

susceptible

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4
Q

Bacitracin:

is Staphylococci susceptible or resistant?

A

Susceptible (check this one)

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5
Q

Sapro can be differentiated from other species of Staphylococcus through antibiotic testing as it is resistant to the antibiotic __________.

A

novobiocin

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6
Q

Novobiocin resistant staph?

A

Saprophyticus

S. Aureus is susceptible

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7
Q

What is the latex agglutination test is for?

A

-protein A and bound coagulase
-possess coagulase and/or protein A, particularly Staphylococcus aureus

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8
Q

how to differentiate between strep pyo and strep agalac?

A

Bacitracin (A-disk)

-Differentiating S. pyogenes from other β-hemolytic Streptococcus spp.

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9
Q

Pyogenes is ___________ to bacitracin

A

susceptible

  • any zone of inhibition of growth is considered a positive test result
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10
Q

Pyogenes is PYR _____ and strep agalac is PYR _______.

A

positive,

negative

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11
Q

CAMP/HHT positive or negative?

-aga: ___________
-pyo: ___________

A

positive

negative

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12
Q

“disk test” is used for the different streps

sus for pneumo?

sus for pyo?

A

P disk (Optochin disk)

A disk (Bacitracin disk)

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13
Q

Lancefield classification:

Pyo?

A

A

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14
Q

Lancefield classification:

aga

A

B

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15
Q

Lancefield classification:

dys?

A

C? G?

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16
Q

Lancefield classification:

entero/non-entero?

A

D

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17
Q

what organisms are DNAse (+)?

A

Aureus and moraxella

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18
Q

True or false:

Strep agalactiae is hippurate negative

A

FALSE, hippurate positive!***

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19
Q

Is neisseria gram positive or negative?
what is the morphology?

A

gram negative diplococci

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20
Q

Know the difference between gonorrhea and meningitidis based on the sugars

-gon =
-men =

A

gon: just glucose

men: glucose and maltose

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21
Q

Know what tests are usually done to identify the different neisseria

A

-Gram stain: Gram negative diplococci
-Oxidase test: positive result
-Antigen detection/serology testing

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22
Q

What is used for definitive diagnosis of different neisseria?

A

nucleic acid amplification testing (NAAT), Agar dilution susceptibility testing

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23
Q

Which neisseria is found in joint fluid?

A

N. Gon

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24
Q

How to identify Gardenella Vaginalis?

A

Analysis of vaginal discharge, pH, and scent

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25
Gardenella Vaginalis: gram stain...
gram-negative coccobacilli
26
Gardenella Vaginalis: "______ cells" can be seen when doing wet mount.
clue
27
Gardenella Vaginalis: What is positive?
glucose/maltose fermentation, Hippurate Hydrolysis
28
Gardenella Vaginalis: What is negative?
urease, nitrate reduction, mannitol fermentation, xylose fermentation
29
Know what media is used for G. vaginalis
Human blood tween
30
Know what the Loeffler medium is used for?
Used for the growth and isolation of the Corynebacterium spp.
31
Know that ____ is characteristic of Erysipelothrix on TSI media***
H2S
32
Erysipelothrix is gram _________
positive
33
What kind of infection is Erysipeolthrix associated with?
skin infections from fish
34
brucella is a _____________ agent.
bioterrorism
35
Could you work with burcella on the bench if you clean with bleach?
NO. bioterrorism agent due to their ability to undergo aerosolization
36
If a brucella culture shows no growth at 5 days, how long can you keep letting it grow?
21 days is the longest
37
best way to incubate campylobacter?
-42*c microaerophilic environment -Media: Campy-BA, Charcoal-based selective medium (CSM), Campylobacter agar base blood-free (CCDA)
38
How to quickly identify B. anthraxis from other bacillus spp
-B. anthracis is typically non-motile and displays gamma hemolysis as well as a susceptibility to penicillin and gamma phages -Can be ruled out through the use of penicillin resistance testing since it is susceptible, motility testing since it is non-motile, and inoculation of blood agar since it is non-hemolytic
39
Is bacillus a frequent contaminant?
They rarely cause disease in the immunocompetent and are more frequently isolated as a culture contaminant
40
Can bacillus grows on MAC?
No, typically Bacillus species cannot grow. MacConkey agar is selective for gram-negative and inhibits the grown of gram positive bacteria -bacillus are gram-positive and do not ferment lactose so they will not grow well on MacConkey agar
41
Differentiate between staph, strep, neisseria: Gram positive chains with catalase positive will be...
staphylococci
42
Staph aureus and intermedius are both coagulase and catalase ______________
positive
43
N. gonorrheae -> ____________ fermenter
glucose
44
N. meningitidis -> ____________ fermenter
Glucose & maltose
45
Generally… Strep -> Catalase ____________ Coagulase __________
neg neg
45
N. Lactamica -> ________________ fermenter
Glucose, Maltose, and Lactose
46
Generally.... Staph -> Catalase and Coagulase ____________
positive ?
47
Micrococcus is _____________ sensitive.
bacitracin
48
Mircococcus is catalase ________.
positive
49
Micrococcus is coagulase ___________.
negative
50
Micrococcus is oxidase ___________.
positive
51
What causes Whooping cough?
Bordetella pertussis or Bordetella parapertussis that is found in the mouth, nose and throat of an infected person
52
What media is used for Bordetella pertussis?
charcoal-blood agar (REGAN-LOWE medium) or Bordet-Gengou agar (primary isolation)
53
Haemophilus: Gram ___________ coccobacillus
negative
54
Haemophilus: motile or non motile?
non motile
55
Haemophilus is normal flora of...
upper respiratory tract
56
Which haemophilus has Beta hemolysis of horse blood?
H. haemolyticus
57
What Haemophilus requires X factor (hemin)?
H. influenza , H. ducreyi, and H. heamolyticus
58
What haemphilus requires V factor (NAD)?
H. influenza, H. haemolyticus, and H. parainfluenzae
59
Which hemophilus has the ability to convert ALA to Protoporphyrin?
H. parainfluenzea, and H. aphrophilus
60
What agars do heamophilus grown on?
all will grow on chocolate and (10% CO2) agar because it supplies both X and V factors -also grow on horse or rabbit blood agar.
61
What agar will haemphilus NOT grow on?
Sheep Blood agar because SBA contains NADase, which inactivates NAD
62
What is the phenomenon known as "satelliting"?
When grown in media containing blood, staphylococcus aureus produces NAD (V factor) as a byproduct. It also hemolyzes blood cells, releasing hemin (factor X) As a result Haemophilus is able to grow on sheep blood agar close to the colonies of S. aureus.
63
What bacteria are bile esculin positive?
Entero and Listeria
64
factor V is released from __________ and aids for growth of H.influence!
staph
65
which brucella is pos on brucella dye tolerance media for BOTH thionin and fuchin?
Brucella melitensis
66
gardnerella uses ________ media AND Hippurate Hydrolysis pos
tween
67
What is the morphology of garnerella?
gram positive bacilli
68
garnernell is catalase...
positive
69
Does garnerella produce H2S?
No
70
Lactobacillus can be confused with garnerella. the main thing is lactobacillus is normal flora and found in _________ and ________.
palisades chains
71
which media to use for C. diphtheriae ID
Loeffler and Cystine Tellurite Blood Agar (CBTA)
72
Micrococcus is catalase _________.
positive
73
Micrococcus is coagulase _________.
negative
74
Mircococcus is _________ sensitive.
Bacitracin
75
Mircooccus is oxidase _______.
positive
76
How does mircooccus utilize sugars in the O/F test?
oxidatively or not at all
77
O/F test: mircococcus oxidatively ---->
yellow w/ open tube & green w/ closed tube
78
O/F test: oxidizer -->
(utilizes sugar not at all) will be green / green
79

Medical Microbiology (12 decks)

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