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Medical Microbiology > Exam A Retake > Flashcards

Exam A Retake Flashcards

1
Q

What agar is used for E. coli O157 pathogenic strain?

A

SMAC (sorbitol + MAC)

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2
Q

What is the appearance of E. coli on EMB?

A

green sheen

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3
Q

What test is used to differntiate E. Coli from Klebsiella?

A

indole (E. coli is pos)

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4
Q

Three organisms that are PAD positive?***

A

-Proteus
-Morganella
-Providencia

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5
Q

What is the only indole positive Klebsiella?

A

K. oxytoca

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6
Q

Klebsiella:

Motility…
MUG…

A

nonmotile

MUG pos

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7
Q

a late lactose fermenter ONPG

A

Citrobacter

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8
Q

o Motile
o Produces red pigment (prodigiosin)

A

Serratia

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9
Q

o Triple negative decarboxylation
o Smooth colonies on blood agar
o Yellow colonies on HEK & XLD

A

Enterobacter

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10
Q

How is Salmonella differentiated from proteus?

A

urease (proteus is pos)

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11
Q

Salmonella:

indole _________
urease ________

A

neg, neg

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12
Q

what is triple pos? (Lysine/Ornithine/ Agrinine)

What is triple neg decarboxylation?

A

Salmonella

Enterobacter

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13
Q

The only Shigella subtype that is ornithine + and ONPG +

A

Subtype C = S boydii***

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14
Q

Proteus is ________ on LIA

A

red***

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15
Q

Proteus vulgaris:

indole _______
citrate _______

A

pos, neg***

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16
Q

Proteus mirabilis:

indole ________
citrate ________

A

neg, pos***

-ornithine decarboxylase +

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17
Q

***Proteus is PAD _____

A

+

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18
Q

Bacteria that can cause otisis media?***

A

S. pneumoniae, Moraxella catarhalis**, and Haemophilus influenzae

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19
Q

Proteus on LIA?

A

red slant on LIA, deaminates Lysine (look at slant)!***

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20
Q

What causes Yersinia petis?

A

by fleas —> rats —> humans
o Think ships, causes bubonic plague
o Bipolar staining

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21
Q

Y. enetero has bullseye colonies on ______

A

CIN

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22
Q

o wound cultures
o H2S +

A

Shewanella

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23
Q

Vibrio cholerae is ________ on TCBS

A

yellow (check this one)

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24
Q

green on TCBS that comes from seafood

A

Vibrio parahaemoluticus ***

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25
Q

Clostridium is gram _____

A

positive

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26
Q

Streptococcus agalactiae:

hippurate _____

A

pos

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27
Q

Streptococcus agalactiae:

bile esculin _____

A

neg

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28
Q

Streptococcus agalactiae:

CAMP ________

A

pos

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29
Q

Streptococcus agalactiae is group ____ step

A

B

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30
Q

Pasteurella:

Gram _____
Oxidase ________

A

neg
pos

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31
Q

Pasteurella motility?

A

non motile

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32
Q

True or false:

Pasteruella cannot grow on MAC?

A

true

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33
Q

What Haemophilus can grow on 3/4 of the quad plate?

A

Haemophilus parainfluenzae

It only needs factor V (NAD), quads II, III and IV have NAD

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34
Q

How is Moraxella differentiated from Neisseria?

A

Moraxella is DNAse +

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35
Q

_________ turns GREEN on TCBS and comes from seafood

A

Vibrio para

36
Q

MSA (Mannitol Salt Agar) plates used to differentiate…

A

staph epi from staph aureus***

Staph epi will be RED

Staph aureus will be YELLOW (pos)

37
Q

Pasteurella causes ________ infections

A

wound (question with this and 3 other choices…picked Pasteurella because it was the only one I recognized)

38
Q

C. diptheria will grow on ________ AND ___________.***

A

Loeffler AND Tellurite

39
Q

For urine counts:

-If the loop size is 0.01, multiply by _____

-If the loop size is 0.001, multiply by _____

A

100

1,000

40
Q

For urine counts:

if the colony count is >_______ you report it as >_______ .

A

100,000

-If it is less than 100,000 it is not significant (it means there is not UTI, and so you don’t need to ID) (check this one, does it need to be ID?)

41
Q

MAC inhibits gram + organisms using _________ and _________.

A

crystal violet and bile salts

42
Q

Campylobacter growth conditions

A

Campy agar, 42C and CO2 incubator

43
Q

Two gram neg. bacteria that are catalase pos?

A

E. coli and Vibrio

44
Q

V shaped, Chinese letter

A

Corynebacterium

45
Q

Gram +
Elek +

A

Corynebacterium

46
Q

o Catalase +
o Cystine tellurite BA or modified tinsdale

A

Corynebacterium

47
Q

o Group A
o Susceptible to bacitracin (A Disk)
o PYR +

A

Streptococcus pyogenes

48
Q

o Group B
o RESISTANT TO BACITRACIN
o CAMP & HHT +

A

Streptococcus agalactiae

49
Q

S. pyogenes, S. agalactiae:

Which is resistant to Bacitracin (A disk)?

A

Streptococcus agalactiae is RESISTANT TO BACITRACIN***

Streptococcus pyogenes is susceptible

50
Q

Neisseria gonorrhea morphology

A

Gram neg diplococci

51
Q

N. Gon.

What sugar does it use?

A

ONLY glucose

52
Q

Media used for Neisseria gonorrhea

A

-Thayer-Martin & Chocolate agar
-If Thayer-martin is not a choice for gram – diplo, then its chocolate

53
Q

Neisseria gonorrhea is oxidase ______

A

pos

54
Q

Which Neisseria can cause joint infection?

A

N. gonorrhea

55
Q

Common cause of meningitis

A

Neisseria meningitis

** if there was no growth for meningitis cultures… it is aseptic meningitis**

56
Q

What are the urease pos organisms?***

A

Proteus, Klebsiella, Serratia, Morganella

(Uncle Pete Knits Sweaters on Monday)

57
Q

Indole pos organisms?

A

-Mogranella
-E coli
-K. oxytoca
-P. vulgaris

58
Q

Arginine Positive:

A

-Enterobacter
-Salmonella (triple pos)
-Citrobacter freudii

59
Q

PAD Positive:

A

PPM
Proteus
Providencia
Morganella

60
Q

K/A TSI:

A

o Salmonella
o Shigella
o Proteus
o Morganella
o Providencia
-Essentially all the ones that do not ferment lactose are K/A
-K/A DO ferment glucose though

61
Q

Lactose Fermenters:

A

o E. coli
o Klebsiella
o Citrobacter
o Enterobacter

62
Q

HEK growth:

Lac + will be ________
Lac - will be ________

A

yellow (enterobacter)

blue/green (shigella/maybe colorless?)

63
Q

What color is salmonella on HEK?

A

black

64
Q

What is in MacConkey agar?

A

-bile salts and crystal violet
-Neutral red indicator

65
Q

MacConkey:

Lac + ________
Lac - ________

A

pink

yellow

66
Q

Xylose Lysine Deoxycholate:

Lac + ______
Lac -_______

A

yellow

red

H2S = black

67
Q

What does XLD isolate?

A

gram -

68
Q

Gram + cocci grow in _________ in Thioglycolate broth

A

puffballs

69
Q

What three medias could be used to isolate salmonella and shigella in stool?

A

-HE
-XLD
-SS

70
Q

What agar is used to isolate E. coli O157?

A

SMAC

71
Q

What color is E. coli on XLD?

A

yellow

72
Q

What color is some proteus species on XLD?

A

yellow with black centers

73
Q

What color is shigella on XLD?

A

colorless or red colonies

74
Q

What color is salmonella on XLD?

A

red with black centers

75
Q

How does salmonella and shigella appear on SS agar?

A

colorless colonies (neither ferment lactose) but salmonella produces black centers

76
Q

TCBS is selective for ________.

A

vibrio

77
Q

Regan-Lowe agar is selective for_________.

A

B. pertussis

78
Q

BCYE is used for isolation of ___________.

A

legionella

79
Q

Bacteria that can cross the placenta and infect the fetus

A

Listeria monocytogenes

80
Q

Neisseria spp.:

catalase ______
oxidase ______

A

pos, pos

81
Q

Motility:

Most enterobacterales are pos, except ___________ and ___________.

A

Klebsiella and shigella

82
Q

What media is used to inhibit normal fecal flora and isolate enteric pathogens?

A

GN broth (not sure)

83
Q

CNA agar is used to isolate ________.

A

Gram positive bacteria

84
Q

How can Klebsiella be differentiated from Enterobacter?

A

Motility (enterobacter is pos)

85
Q

How can Shigella be differentiated from Salmonella?

A

Motility (salmonella is pos)

86
Q
A
87
Q

The string test can be used to differentiate between…

A

Vibrio cholerae (positive) and
other Vibrio spp (negative)

Medical Microbiology (12 decks)

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