Exam A Retake Flashcards by Sarah King

What agar is used for E. coli O157 pathogenic strain?

SMAC (sorbitol + MAC)

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What is the appearance of E. coli on EMB?

green sheen

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What test is used to differntiate E. Coli from Klebsiella?

indole (E. coli is pos)

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Three organisms that are PAD positive?***

-Proteus
-Morganella
-Providencia

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What is the only indole positive Klebsiella?

K. oxytoca

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Klebsiella:

Motility…
MUG…

nonmotile

MUG pos

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a late lactose fermenter ONPG

Citrobacter

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o Motile
o Produces red pigment (prodigiosin)

Serratia

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o Triple negative decarboxylation
o Smooth colonies on blood agar
o Yellow colonies on HEK & XLD

Enterobacter

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How is Salmonella differentiated from proteus?

urease (proteus is pos)

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Salmonella:

indole _________
urease ________

neg, neg

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what is triple pos? (Lysine/Ornithine/ Agrinine)

What is triple neg decarboxylation?

Salmonella

Enterobacter

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The only Shigella subtype that is ornithine + and ONPG +

Subtype C = S boydii***

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Proteus is ________ on LIA

red***

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Proteus vulgaris:

indole _______
citrate _______

pos, neg***

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Proteus mirabilis:

indole ________
citrate ________

neg, pos***

-ornithine decarboxylase +

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***Proteus is PAD _____

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Bacteria that can cause otisis media?***

S. pneumoniae, Moraxella catarhalis**, and Haemophilus influenzae

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Proteus on LIA?

red slant on LIA, deaminates Lysine (look at slant)!***

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What causes Yersinia petis?

by fleas —> rats —> humans
o Think ships, causes bubonic plague
o Bipolar staining

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Y. enetero has bullseye colonies on ______

CIN

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o wound cultures
o H2S +

Shewanella

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Vibrio cholerae is ________ on TCBS

yellow (check this one)

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green on TCBS that comes from seafood

Vibrio parahaemoluticus ***

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Clostridium is gram _____

positive

Streptococcus agalactiae: hippurate _____

pos

Streptococcus agalactiae: bile esculin _____

neg

Streptococcus agalactiae: CAMP ________

pos

Streptococcus agalactiae is group ____ step

Pasteurella: Gram _____ Oxidase ________

neg pos

Pasteurella motility?

non motile

True or false: Pasteruella cannot grow on MAC?

true

What Haemophilus can grow on 3/4 of the quad plate?

Haemophilus parainfluenzae It only needs factor V (NAD), quads II, III and IV have NAD

How is Moraxella differentiated from Neisseria?

Moraxella is DNAse +

_________ turns GREEN on TCBS and comes from seafood

Vibrio para

MSA (Mannitol Salt Agar) plates used to differentiate...

staph epi from staph aureus*** Staph epi will be RED Staph aureus will be YELLOW (pos)

Pasteurella causes ________ infections

wound (question with this and 3 other choices…picked Pasteurella because it was the only one I recognized)

C. diptheria will grow on ________ AND ___________.***

Loeffler AND Tellurite

For urine counts: -If the loop size is 0.01, multiply by _____ -If the loop size is 0.001, multiply by _____

100 1,000

For urine counts: if the colony count is >_______ you report it as >_______ .

100,000 -If it is less than 100,000 it is not significant (it means there is not UTI, and so you don’t need to ID) (check this one, does it need to be ID?)

MAC inhibits gram + organisms using _________ and _________.

crystal violet and bile salts

Campylobacter growth conditions

Campy agar, 42C and CO2 incubator

Two gram neg. bacteria that are catalase pos?

E. coli and Vibrio

V shaped, Chinese letter

Corynebacterium

Gram + Elek +

Corynebacterium

o Catalase + o Cystine tellurite BA or modified tinsdale

Corynebacterium

o Group A o Susceptible to bacitracin (A Disk) o PYR +

Streptococcus pyogenes

o Group B o RESISTANT TO BACITRACIN o CAMP & HHT +

Streptococcus agalactiae

S. pyogenes, S. agalactiae: Which is resistant to Bacitracin (A disk)?

Streptococcus agalactiae is RESISTANT TO BACITRACIN*** Streptococcus pyogenes is susceptible

Neisseria gonorrhea morphology

Gram neg diplococci

N. Gon. What sugar does it use?

ONLY glucose

Media used for Neisseria gonorrhea

-Thayer-Martin & Chocolate agar -If Thayer-martin is not a choice for gram – diplo, then its chocolate

Neisseria gonorrhea is oxidase ______

pos

Which Neisseria can cause joint infection?

N. gonorrhea

Common cause of meningitis

Neisseria meningitis ** if there was no growth for meningitis cultures… it is aseptic meningitis**

What are the urease pos organisms?***

Proteus, Klebsiella, Serratia, Morganella (Uncle Pete Knits Sweaters on Monday)

Indole pos organisms?

-Mogranella -E coli -K. oxytoca -P. vulgaris

Arginine Positive:

-Enterobacter -Salmonella (triple pos) -Citrobacter freudii

PAD Positive:

PPM Proteus Providencia Morganella

K/A TSI:

o Salmonella o Shigella o Proteus o Morganella o Providencia -Essentially all the ones that do not ferment lactose are K/A -K/A DO ferment glucose though

Lactose Fermenters:

o E. coli o Klebsiella o Citrobacter o Enterobacter

HEK growth: Lac + will be ________ Lac - will be ________

yellow (enterobacter) blue/green (shigella/maybe colorless?)

What color is salmonella on HEK?

black

What is in MacConkey agar?

-bile salts and crystal violet -Neutral red indicator

MacConkey: Lac + ________ Lac - ________

pink yellow

Xylose Lysine Deoxycholate: Lac + ______ Lac -_______

yellow red H2S = black

What does XLD isolate?

gram -

Gram + cocci grow in _________ in Thioglycolate broth

puffballs

What three medias could be used to isolate salmonella and shigella in stool?

-HE -XLD -SS

What agar is used to isolate E. coli O157?

SMAC

What color is E. coli on XLD?

yellow

What color is some proteus species on XLD?

yellow with black centers

What color is shigella on XLD?

colorless or red colonies

What color is salmonella on XLD?

red with black centers

How does salmonella and shigella appear on SS agar?

colorless colonies (neither ferment lactose) but salmonella produces black centers

TCBS is selective for ________.

vibrio

Regan-Lowe agar is selective for_________.

B. pertussis

BCYE is used for isolation of ___________.

legionella

Bacteria that can cross the placenta and infect the fetus

Listeria monocytogenes

Neisseria spp.: catalase ______ oxidase ______

pos, pos

Motility: Most enterobacterales are pos, except ___________ and ___________.

Klebsiella and shigella

What media is used to inhibit normal fecal flora and isolate enteric pathogens?

GN broth (not sure)

CNA agar is used to isolate ________.

Gram positive bacteria

How can Klebsiella be differentiated from Enterobacter?

Motility (enterobacter is pos)

How can Shigella be differentiated from Salmonella?

Motility (salmonella is pos)

The string test can be used to differentiate between…

Vibrio cholerae (positive) and other Vibrio spp (negative)

Exam A Retake Flashcards

(87 cards)