Examination of Fresh Tissue and Fixation Flashcards
(76 cards)
The microscopic study of the normal tissues of the body
Histology
The microscopic study of tissues affected by disease
Histopathology
The simplest, least invasive test and uses the smallest needle to simply remove cells from the area of abnormality.
Fine needle aspiration
This is not always adequate to obtain a diagnosis, depending on the area being biopsied
Fine needle aspiration
Removes not only cells, but also a small portion of the surrounding tissue. This provides additional information to assist in the examination of the lesion.
Core needle biopsy
Takes out even more surrounding tissues. It takes some out of the abnormality, but noy all. The doctor will slice into the lesion and remove only a portion of it. If the lesion is found to be cancerous, further surgery may be needed to remove the lesion.
Incisional biopsy
Generally removes the entire area in question
Excisional biopsy
Considered to be the primary technique for obtaining diagnostic full-thickness skin specimens
Punch biopsy
The technique involves the use of a circular blade that is roated down through the epidermis and dermis, and into the subcutaneous fat, yielding a 3-4 mm cylindrical core of the tissue sample.
Punch biopsy
Where small fragments of tissue are shaved from a surface (usually a skin)
Shave biopsy
Where tissue is scooped or spooned to remove tissue or growth from body cavity such as endometrium or cervical canal
Curettings
specimens that are usually examined when there is an immediate need for evaluation
Fresh tissues
Is a process whereby a selected tissue specimen is immersed in isotonic salt solution such as normal saline solution or roger’s solution in a petri dish or watch glass, carefully dissected with a needle and separated by direct or zigzag spread using an applicator stick.
Teasing or or dissociation
In teasing or dissociation, the specimen is either stained with _____ or examined unstained by _______
supravital
phase-contrast microscopy
Is a process whereby small pieces of tissue (not more than one mm in diameter) are placed in a microscopic slide and forcibly compressed with another slide or with a cover glass.
Squash preparation (Crushing)
In squash preparation (crushing), a supravital stain if necessary, may be placed at the junction of the slide and the cover glass, and allowed to be absorbed by the tissue through _____
capillary attraction
the method of preparing the smears differs depending on the nature of the material to be examined.
Smear preparation
These may be examined as either as fresh prep similar to that described for teased preparations, or by using a supravital technique.
Smear
This is useful for preparing smears of thick secretions like mucous fluids, serous fluids, sputum, enzymatic lavage samples from GI tract, and blood smears
Smear preparation
This is useful for preparing smears of thick secretions like mucous fluids, serous fluids, sputum, enzymatic lavage samples from GI tract, and blood smears
Smear preparation
With an applicator stick or an platinum loop, the material is rapidly and gently applied in a direct or zigzag line throughout the slide, attempting to obtain a relatively uniform distribution.
Streaking
A selected portion of the material is transferred into a clean slide and gently spread into moderately thick film by teasing the mucous strands with applicator stick.
Spreading
It is little more tedious than streaking, but has many advantage of maintaining cellular interrelationships of the material to be examined
Spreading
It is specially recommended for smear preparation of fresh sputum and bronchial aspirates, and also for thick mucoid secretions
Spreading
