Exercise 20 - The Staphylococci Flashcards Preview

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Flashcards in Exercise 20 - The Staphylococci Deck (18)
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0

Gram stain reaction, size, shape and arrangement of staph?

Purple color

Large cocci

Staphylo

1

Which major tests are used to distinguish the different species of staph from each other

A-toxin

Mannitol

Coagulase

DNase

Novobiocin

2

Know the names, purpose, ingredients, results of all media (study the chart)

Yea

3

What is the biochemical pattern for Staph Aureus

Beta hemolysis (alpha toxin)

Mannitol fermentation

Coagulase production

Positive for all of these tests

4

Which test was used to separate S. Saprophyticus form the other species

Novobiocin

5

Be able to read all the media for + or - reactions

Yuh

6

Mannitol salt agar:

What's its selective ingredient

What's its deferential ingredients

What does it select for

7.5% NaCl

Mannitol and phenol red

Selects for halophiles and halotolerants

7

Mannitol salt agar:

What is its purpose

What does it look like if it does ferment mannitol, if it doesn't

Test for Mannitol fermentation, differentiate staph species

+ yellow (acid changing phenol red to yellow)

- red

8

SM110 agar staphylococcus media:

What are its selective ingredients

What are its differential ingredients

What does it select for

7.5% NaCL

Mannitol

Halophiles and Halotolerant

9

SM110 agar staphylococcus media:

What is its purpose?

What will Staph A look like, and CNS?

Differentiate staph species

Staph A will be a golden color

CNS will be white/opaque color

10

What does CNS stand for?

Coagulase Negative Staph

11

Blood agar:

What are its differential ingredients

What does its enrichment select for?

Hemoglobin

Fastidious bacteria

12

Blood agar:

What is its purpose

What do beta, alpha, gamma look like

Hemolytic patterns used in Staph/strep ID

Beta- clear agar
Alpha- green agar
Gamma- no change

13

Rabbit plasma:

What type of test is this?

What are its ingredients?

Differential

Proteins for clotting

14

Rabbit plasma:

What is its purpose

What do positive and neg results look like

Coagulase test differentiate Staph. A vs CNS

+ clot forms solid/semisolid = Staph. A

- no clot forms, liquid = CNS species

15

Mueller Hinton Agar:

Novobiocin:

What is its purpose?

What do the results mean?

Differentiate Staphylococcus saprophyticus from other staph

Zone of inhibition = greater than 16 S (others)
Zone of inhibition = less than 16 R - Staphylococcus saprophyticus

16

m-Staphylococcus Broth:

What type of test is this?

What are its ingredients

What does it select for

Selective, enrichment

S - 10% NaCL
E - for staphylococcus

Selects for halophiles and Halotolerant

17

m-Staphylococcus Broth:

What Is its purpose

What do positive and neg results look like

Encourage growth of Staphylococcus; eliminate non-staph species

+ = growth
- = no growth