Exercise 24 - PCR Detection Flashcards Preview

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Flashcards in Exercise 24 - PCR Detection Deck (11)
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What does PCR stand for

Polymerase chain reaction

1

What is the theory behind PCR, what does it accomplish

Diagnostic applications of PCR include detection of antibiotic resistant strains

Could tell you the difference between MRSA and non resistant Staph. A

2

What is the procedure for PCR

Take a gram + cocci

Transfer to MSA plate if positive continue to coagulase test

If coagulase positive, move to Oxacillin plate test or polymerase chain reaction test

3

What are the events of PCR

1. Denaturation 94 C
2. Annealing (forward primer, reverse primer)
3. Extension (copy)

4

Origin and purpose of Taq DNA polymerase

DNA polymerase is from Thermophilus aquaticus

Taq won't denature in high heat

5

What is the purpose of primers

To copy the DNA

6

Procedure for agarose gel electrophoresis

Uses micro pipettes to put in holes in the gel.

Turn on machine, DNA moves toward positive charge

7

How do you. Locate the mecA gene on a finished AGE

Produces size 533 base pairs

8

What size band identifies the mecA gene of a finished AGE

533bp band

9

What are the advantages and disadvantages of using the PCR method instead of Kirby Bauer method

PCR is faster, but more expensive

10

What protein is produced by the mecA gene?

What ability doe it give the Staphylococci

PB2 - penicillin binding protein # 2

Offers resistance to cillin antibiotics