Exercise 19 - Antibiotic Sensitivity Testing Flashcards Preview

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Flashcards in Exercise 19 - Antibiotic Sensitivity Testing Deck (8)
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0

What is the name of the media used in the Kirby Bauer method

Mueller-Hinton agar

1

How are the bacteria plated on the media? Lawn?

Spread all over the plate with a q-tip

Or you lawn them with the metal triangle instrument

2

What is the general procedure of the experiment in exercise 19

1. Swab the entire surface of petri with a bacteria

2. Place antibiotic disks on the agar

3. After incubation, measure zones of inhibition

3

Zones of Inhibition: how are the measurements taken and which metric units are the answers expressed in?

With a ruler measuring the diameter of zone of inhibition

mm

4

Know how to read a chart of antibiotics listing the zone diameters and correctly designate the bacteria to be: R, I, S to that antibiotic

Pg 120

5

What does R, I, S stand for

Resistant

Sensitive

Intermediate

6

Which bacteria is the most resistant to the antibiotics?

Pseudomonas aerugosa

7

Which bacteria was the most sensitive to antibiotics?

Staphylococcus aureus