Exercise 8 - Observing Plant Growth and Development

Question 1

A typical plant consists of (3)

Answer 1

embryonic organs

Developing organs

Mature organs

Question 2

_____ the irreversible increase in mass that results from ____ and _____

Answer 2

Growth

cell division (number)
cell expansion (size)

Question 3

What produces the plant body? (3)

Answer 3

Growth, morphogenesis, and differentiation

Question 4

____ the development of body form and organization, including recognizable tissues and organs

Answer 4

morphogenesis

Question 5

____ The specialization of cells with the same set of genetic instructions to produce a diversity of cell types is called

Answer 5

differentiation

Question 6

____ in ____, by increasing cell number, increases the potential for growth.

However, it is ____ that accounts for the actual increase in plant mass.

Together these processes contributeto plant form.

Answer 6

Cell division
Meristem

Cell Expansion

Question 7

Parts of Exercise 8 - Observing Plant Growth and Development

A.

B.

Answer 7

A. Preparation of OD Medium

B. Embryo rescue of Zea mays L.

Question 8

A. Preparation of OD medium

Materials (4):

Answer 8

2g NPK Fertilizer

150 mL Fresh Coconut Water

30g Sucrose (White Sugar)

15g agar

Question 9

A. Preparation of OD medium

Procedure:
1. Weigh out ____g of NPK fertilizer mixture and dissolve in ____mL of distilled water.

2. Add ____mL fresh coconut water and ____g of sucrose
3. Top up the volume into ____ mL
4. Maintain pH at _____
5. Add ____g agar and head it up till the entire agar dissolves

Answer 9

A. Preparation of OD medium

1. 2.0g NPK, 10 mL distilled water
2. 150 mL fresh coconut water, 30g sucrose
3. 995 mL water
4. 5.6 pH
5. 15g Agar

Question 10

A. Preparation of OD medium

6. Top up the volume of ___ L if it is less than that.
7. Put ____ mL of the medium into each ____
8. Cover the tubes with _____ or _____.
9. Put ____ tightly around the cover to seal it.
10. ____ it as we do in Microbiology Laboratory Class

Answer 10

A. Preparation of OD medium

6. 1L water
7. 10 mL of medium, boiling tube
8. Polypropylene, Autoclavable materials
9. Rubber bands
10. Autoclave

Question 11

B. Embryo rescue of Zea mays L.

Materials:

Answer 11

Immature corn cob

70% Ethanol

95% Ethanol

Liquid Soap

Question 12

B. Embryo rescue of Zea mays L.

Procedure:

1. Using scalpel, carefully remove intact _____
2. Wash then under running water for ____ mins
3. Then surface sterilize the seeds using the method given below:
   -_____ for 15 minutes

-Transfer into a laminar flow cabiner (Should be cleaned and sprayed with alcohol and then switched on for 30 mins before use)

• Wash _____ times in sterile ____ for 2 mins
• Wash _____ times in sterile ____

Answer 12

1. Young corn kernels
2. 10 mins
3. 10% chlorine bleach
   3 times sterile 70% ethanol
   3 times sterile distilled water

Question 13

B. Embryo rescue of Zea mays L.

Procedure:
4. Dip forceps in ____ then flame shortly

5. Cut off the ____ of kernel and excise _____ using the scalpel
6. Aseptically transfer the ____ to_____
7. Place the ____ in the ____ with 25 +-1 C for growth

Answer 13

4. 95% ethanol
5. hard base
   embryo
6. Embryo
   OD medium
7. inoculated tubes
   growth room

Question 14

B. Embryo rescue of Zea mays L.

Procedure:
8. Observes the cultures regularly
- Note the day when ____ and ____ begin to emerge from the embryos
- Note number of embryos and contaminated culture, if present

9. Observe for 3 weeks
10. Observe daily and record your observation

Answer 14

8. Shoots and Roots

Question 15

Parts of observation (6)

Answer 15

day of germination

day of root emergence

length of root in cm

length of sheet

number of leaves

Size of leaves