Experiment 3 Flashcards
Reagent hat is used to react with starch
Lugol’s Solution
Other name of Lugol’s Solution
Potassium Iodide (KI)
What happens when the Lugol’s solution comes into contact with starch
Light amber to Blue-black solution
What reagent is used to react with Protein
Biuret Solution
Other name for Biuret Solution
Carbamylurea
How to identify a positive control with the Biuret solution
Blue-Violet solution
What reagent is used for identifying Lipids
Sudan IV
Other name for Sudan IV
Lysochrome fat soluble dye
Chemical formula of Sudan IV
C24H20N4O
What happens when Sudan IV comes into contact with lipids
Deep-colored solution
H2O + Lugol’s solution
No reaction (-)
Glucose + Lugol’s solution
No reaction (-), red to yellow-colored solution
Chewed Biscuits + Lugol’s solution
(+) Blue-Black
Milk + Lugol’s solution
No reaction (-), * sometimes has starch
Egg Albumin + Lugol’s solution
No reaction (-)
vegetable Oil + Lugol’s solution
No reaction (-)
Why does chewed biscuit have a positive reaction with Lugol’s solution
Because of amylase
H2O + Biuret Solution
No reaction (-)
Glucose + Biuret Solution
No reaction (-) Brick red
Chewed Biscuits + Biuret Solution
No reaction (-)
Milk + Biuret Solution
(+) Purple solution
Egg albumin + Biuret Solution
(+) Violet solution
Vegetable oil + Biuret Solution
No reaction (-)
H2O + Sudan IV
No reaction (-)
Glucose + Sudan IV
No reaction (-)
Chewed Biscuits + Sudan IV
No reaction (-)
Milk + Sudan IV
(+) with fats, no reaction (-) non-fat
Egg + Sudan IV
No reaction (-)
*if egg yolk + Sudan IV (+)