F, Lec (campylobacter20) Flashcards
(37 cards)
Campylobacter spp. have been known to cause
abortion in
domestic animals
Campylobacter is the most common cause of _______ worldwide
Bacterial gastroenteritis
most common
cause of bacterial gastroenteritis worldwide is
Campylobacter
jejuni
transmission of campylobacterioses has been attrib-
uted to
direct contact with animals and handling infected pets
autoimmune disorder characterized by acute
paralysis
Guillain-barre syndrome
recognized as a major cause of type B gas-
tritis,
Helicobacter pylori
chronic condition formerly associated primarily with
stress and chemical irritants
type B gas-
tritis
There is speculation that long-term H. pylori infection
resulting in chronic gastritis is an important risk factor for
gastric
carcinoma
can be recovered in several routine blood
culture media
C. fetus subsp. fetus
Campylobacter spp. that cause enteric illness are
isolated from
stool samples and rectal swabs
toxic to enteric campy-
lobacters and should therefore be avoided.
buffered glycerol saline
Tissue samples may also be
placed in
cysteine-Brucella broth with 20% glycerol and frozen
at −70° C
commonly used medium
enriched selective agar, Campy-BAP
commercially available medium contains
Brucella agar base, 10% sheep red blood cells, and a combination
of antimicrobials—vancomycin, trimethoprim, polymyxin B,
amphotericin B, and cephalothin
H. pylori can be recovered from
gastric biopsy
Other selective media that have
been successful in recovering Campylobacter spp.
Butzler
medium and Skirrow’s medium.
It is important that
the inoculated medium be fresh and moist and that the culture be
incubated in a
Microaerophilic and capnophilic environment
Temp to recover campylobacter jejuni
42C
Temp to recover campylobacter fetus
37C
The ideal atmo-
spheric environment for campylobacter
gas mixture of 5%
O2, 10% CO2, and 85% N2
The ideal atmo-
spheric environment for helicobacter
5% to
10% O2 and 5% to 12% CO2
Microscopic Morphology. Campylobacter spp
curved,
non–spore-forming, gram-negative rods
Enteric campylobacters may appear as
long spirals or ‘S’ or seagull-wing
shapes
recommended as a counterstain
carbolfuchsin