F, Lec (Vibrio20)

Question 1

Found in a wide variety of aquatic environments

Answer 1

Vibrio

Question 2

Most likely to be encountered in the clinical lab

Answer 2

Vibrio cholerae, vibrio parahaemolyticus, vibrio vulnificus, vibrios alginolyticus

Question 3

indication of a
possible Vibrio infection is the presence of recognized factors,
such as

Answer 3

•Recent consumption of raw seafood (especially oysters)
•Recent immigration or foreign travel
•Gastroenteritis with cholera-like or rice-water stools
•Accidental trauma incurred during contact with fresh,
estuarine, or marine water or associated products (e.g.,
shellfish, oyster or clam shells, fishhooks)

Question 4

Shaped of vibrio spp

Answer 4

Curved, comma shaped

Question 5

Are asporogenous, gram neg rods

Answer 5

Vibrio spp

Question 6

Posses polar, sheathed flagella when growing in?

Answer 6

Broth

Question 7

Peritrichous, unsheathed flagella when grown on

Answer 7

Solid media

Question 8

Vibrio are catalase and oxidase

Answer 8

Positive

Question 9

Vibrio can reduce nitrate to nitrite except for

Answer 9

Vibrio metschnikovii

Question 10

Mist vibrio spp are susceptible to

Answer 10

Vibriostatic compound

Question 11

Most vibrios exhibit

Answer 11

Positive string test

Question 12

All species except for vibrio cholera and vibrio mimicus are

Answer 12

Halophilic

Question 13

All spp are halophilic except for

Answer 13

Vibrio cholerae and vibrio mimicus

Question 14

Three major subgroup of vibrio cholerae

Answer 14

V. cholerae O1, V. cholerae O139, and V. cholerae non-O1

Question 15

O1 organisms are divided into the following serotypes:

Answer 15

Ogawa (A, B), Inaba (A, C), and Hikojima (A, B, C).

Question 16

associated with epidemic cholera.

Answer 16

V. cholerae O1 and V. cholerae O139

Question 17

Causitive agent of cholera

Answer 17

Vibrio cholerae O1

Question 18

Vibrio cholerae is also known as

Answer 18

Asiatic cholera and epidemic cholera

Question 19

acute diarrheal disease spread mainly through contaminated water.

Answer 19

Cholera

Question 20

The stools produced by
patients with cholera are described as

Answer 20

Rice water stools

Question 21

The devastating clinical scenario is the result of a powerful
enterotoxin known as

Answer 21

cholera toxin, or choleragen

Question 22

A1 subunit stimulates the
production of

Answer 22

adenylate cyclase

Question 23

A1 subunit stimulates the
production of adenylate cyclase through the inactivation of G
protein. This leads to an accumulation of

Answer 23

cyclic adenosine mono-
phosphate (cAMP)

Question 24

Treatment for cholera

Answer 24

Intravenous or oral fluids

Question 25

V. cholerae O1 strains occur in two biogroups

Answer 25

Classic and El Tor

Question 26

predominant biogroup in the last two pandemics

Answer 26

El Tor

Question 27

El Tor can agglutinate

Answer 27

Chicken red blood cells

Question 28

Voges-Proskauer-positive, hemolyzes erythrocytes, is inhibited by polymyxin B (50 µg),

Answer 28

El Tor

Question 29

second most common Vibrio species implicated in gastroenteritis after V. cholerae.

Answer 29

Vibrio parahaemolyticus

Question 30

Vibrio parahaemolyticus is also called what in japan

Answer 30

Summer diarrhea

Question 31

Vibrio parahaemolyticus is usually associated with

Answer 31

Marine animals

Question 32

T or F: Vibrio parahaemolyticus is self limited

Answer 32

True

Question 33

possible association between hemolysin production and virulence, known as the

Answer 33

Kanagawa phenomenon

Question 34

V. parahaemolyticus strains produce

Answer 34

Heat stable hemolysin

Question 35

second most common Vibrio species implicated in gastroenteritis after V. cholerae

Answer 35

Vibrio parahaemolyticus

Question 36

V parahaemolyticus is also called what in japan

Answer 36

Summer diarrhea

Question 37

V parahaemolyticus is associated with

Answer 37

Marine animals

Question 38

T or F v parahaemolyticus is not self limited

Answer 38

False

Question 39

The pathogenesis of V. parahaemolyticus is not as well under- stood as V. cholerae. However, there is a possible association between hemolysin production and virulence, known as the

Answer 39

Kanagawa phenomenon

Question 40

V. parahaemolyticus strains produce a ________ that is able to lyse human erythrocytes in a special, high-salt mannitol medium (Wagatsuma agar). These strains are considered Kana- gawa toxin-positive,

Answer 40

heat-stable hemolysin

Question 41

V. parahaemolyticus strains produce a heat-stable hemolysin that is able to

Answer 41

lyse human erythrocytes

Question 42

V. parahaemolyticus strains produce a heat-stable hemolysin that is able to lyse human erythrocytes in a special,

Answer 42

high-salt mannitol medium (Wagatsuma agar)

Question 43

V. parahaemolyticus strains produce a heat-stable hemolysin that is able to lyse human erythrocytes in a special, high-salt mannitol medium (Wagatsuma agar). These strains are considered

Answer 43

Kana- gawa toxin-positive

Question 44

second most serious types of Vibrio-associated infections are those caused by

Answer 44

Vibrio vulnificus

Question 45

vulnificus generally fall into two categories

Answer 45

primary septicemia and wound infections.

Question 46

least pathogenic for humans and is the one most infrequently isolated

Answer 46

V. alginolyticus

Question 47

T or f V. alginolyticus is strict halophile

Answer 47

True

Question 48

T or f V. alginolyticus cant be obtained from occupation

Answer 48

False

Question 49

T or f vibrios are not fastidious

Answer 49

True

Question 50

Not recommended for transport of vibrios

Answer 50

Buffered glycerol saline

Question 51

Buffered glycerol saline is not recommended as a transport why

Answer 51

Toxic

Question 52

most commonly used laboratory media,

Answer 52

salt concentration (0.5%)

Question 53

On _____ vibrios produce medium to large colonies that appear smooth, opaque, and iridescent with a greenish hue

Answer 53

SBA or choco- late (CHOC) agar

Question 54

On ______ pathogenic vibrios usually grow as nonlactose fermenters.

Answer 54

MAC

Question 55

Recommended agar

Answer 55

fate citrate bile salt sucrose (TCBS) agar

Question 56

Sucrose fermenting color of colonies on fate citrate bile salt sucrose (TCBS) agar

Answer 56

Yellow

Question 57

Nonsucrose fermenting color of colonies on fate citrate bile salt sucrose (TCBS) agar

Answer 57

Green

Question 58

fate citrate bile salt sucrose (TCBS) agar differentiates what

Answer 58

Sucrose fermenting from nonsucrose fermenting

Question 59

Susceptible to O/129 positive string test

Answer 59

Aeromonas

Question 60

Inability to ferment inositol

Answer 60

Plesiomonas

Question 61

Positive oxidase tesr

Answer 61

Enterobeacteriaceae

Question 62

Fermentative

Answer 62

Pseudomonas