FINAL 02 - Qualitative Tests for Carbohydrates Flashcards

(59 cards)

1
Q

Molisch’s test (Test for)

A

General test for carbohydrates

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2
Q

Molisch’s test (Composition)

A

α-Naphthol

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3
Q

Molisch’s test (Positive result)

A

Purple/red ring

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4
Q

Molisch’s test (Principle)

A

Dehydration

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5
Q

Molisch’s test produces a derivative of furfural known as __________

A

5-hydroxymethylfurfural

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6
Q

Fehling’s test (Test for)

A

Reducing sugars

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7
Q

Fehling’s test A (Composition)

A

CuSO4.5H2O (Copper sulfate pentahydrate)

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8
Q

Fehling’s test B (Composition)

A

K Na Tartrate

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9
Q

Fehling’s test (Positive result)

A

Brick red ppt

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10
Q

Fehling’s test (Principle)

A

Reduction

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11
Q

Benedict’s test (Test for)

A

Reducing sugars

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12
Q

In Benedict’s test, __________ provides the alkaline conditions which are required for the redox reaction

A

Sodium carbonate

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13
Q

In Benedict’s test, __________ complexes with the copper (II) ions so that they do not deteriorate to copper (I) ions during storage

A

Sodium citrate

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14
Q

Benedict’s test (Composition)

A

NaOH + CuSO4

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15
Q

Benedict’s test (Positive resut)

A

Brick red ppt

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16
Q

Benedict’s test (Principle)

A

Reduction

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17
Q

Barfoed’s test (Test for)

A

Monosaccharides

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18
Q

In Barfoed’s test, monosaccharides usually react in about __________ to __________ minutes

A

1-2 minutes

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19
Q

In Barfoed’s test, disaccharides take a much longer time to react with the reagent, about __________ to ___________ minutes

A

7-12 minutes

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20
Q

Barfoed’s test (Composition)

A

Cupric acetate + Acetic acid

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21
Q

Barfoed’s test (Positive result)

A

Brick red ppt

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22
Q

Barfoed’s test (Principle)

A

Reduction

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23
Q

Tollen’s test (Test for)

A

Aldehydes

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24
Q

Tollen’s test (Composition)

A

Silver + Ammonia

25
Tollen's test (Positive result)
Silver mirror
26
Tollen's test (Principle)
Redox
27
Picric acid test (Test for)
Reducing sugars
28
Picric acid test (Composition)
Picric acid
29
Picric acid test (Positive result)
Mahogany red
30
Picric acid test (Principle)
Reduction
31
In picric acid test, the reducing sugars react with picric acid, also chemically known as __________, to form red colored picramic acid
2,4,6-trinitrophenol (TNP)
32
In picric acid test, the reducing sugars react with picric acid, also chemically known as 2,4,6-trinitrophenol (TNP), to form red colored __________
Picramic acid
33
Nylander's test (Test for)
Reducing sugars
34
Nylander's test (Composition)
Na K tartrate + K/NaOH + Bi
35
Nylander's test (Positive result)
Brown
36
Nylander's test (Principle)
Reduction
37
Osazone/Kowarsky test (Test for)
Mannose
38
Osazone/Kowarsky test (Composition)
Phenylhydrazine
39
Osazone/Kowarsky test (Positive result)
Yellow ppt
40
Osazone/Kowarsky test (Principle)
Reduction
41
Seliwanoff's test (Test for)
Ketones/Ketohexoses (Fructose)
42
Seliwanoff's test (Composition)
Resorcinol in conc. HCl
43
Seliwanoff's test (Positive result)
Red/Pink
44
Seliwanoff's test (Principle)
Dehydration
45
Keller-Killiani test (Test for)
Deoxysugars (Cardiac glycosides)
46
Keller-Killiani test (Composition)
Acetic acid, Fe chloride, Sulfuric acid
47
Keller-Killiani test (Positive result)
Reddish brown
48
Keller-Killiani test (Principle)
Hydrolysis
49
Mucic acid test (Test for)
Galactose
50
Mucic acid test (Composition)
Nitric acid
51
Mucic acid test (Positive result)
Insoluble ppt
52
Mucic acid test (Principle)
Oxidation
53
Moore's test (Test for)
Glucose
54
Moore's test (Composition)
Heat with alkali
55
Moore's test (Positive result)
Caramel
56
Bial's test (Test for)
Pentoses
57
Bial's test (Composition)
Resorcinol + Fe
58
Bial's test (Positive result)
Blue green
59
Bial's test (Principle)
Dehydration