Final Exam (Lecture 19) - IgE Mediated Diseases

Question 1

Intradermal allergy test:

What is injected into the dermis (general terms)?

Answer 1

Antigens (allergens)

Question 2

Intradermal allergy test:

What happens when an animal has an IgE-mediated allergy to what is injected?

Why does that happen?

Answer 2

Mast cells degranulate because antigen binds to mast cells.

Question 3

Intradermal allergy test:

List one or two molecules causing the clinical appearance in the skin?

Answer 3

Histamine and leukotrienes

Question 4

Intradermal allergy test:

What do veterinarians call the clinical appearance?

Answer 4

Wheal and flare

Question 5

Intradermal allergy test:

What is used as the positive control?

Answer 5

Histamine

Question 6

Draw an ELISA for detecting IgE-mediated allergy to ragweed.

Answer 6

DONE

Question 7

Compare these therapies, including target and specificity:
1) Apoquel (oclacitinib)
2) Cytopoint (lokivetmab)
3) Allergen-specific immunotherapy (ASIT)

Answer 7

1) Apoquel = Targets the JAK STAT pathway; not very specific as it blocks the kinase pathway used by several cytokines.

2) Cytopoint = Monoclonal antibody to IL-31; very specific as it targets IL-31 specifically and basically neutralizes it so that it cannot bind to the receptor.

3) Introducing allergen to initiate and immune response; desensitizing animal to the antigen

Question 8

Explain one proposed mechanism for the success of allergen-specific immunotherapy (ASIT).

Answer 8

Sublingual:
- Allergen is absorbed through the oral mucosa
based on Treg cell response and B cells making IgA

Question 9

Briefly explain the meaning of the outside-inside-outside phrase in dermatology.

Answer 9

Combination of both inside-outside and outside-inside theory.
- The approach is to control both the inside and
outside factors to minimize allergic reactions