Fixatives Flashcards
(30 cards)
Maintains color - Museum fixative
10% Formol Saline
Recommended for routine post-mortem material
Formol Sublimate
Recommended for lipid fixation
Formol Calcium; Lillie; 1965
Used for glycogen
Genre’s fluid
Used in electron microscopy, fixing material for ultrathin sections for electron microscopy
Osmium tetroxide
Metallic fixatives:
Mercuric fixatives
Chromate fixatives
Lead fixatives
Ions of this acts by combining with acidic (carboxyl -COOH) groups of proteins and form especially strong combination with the sulfur (thiol) radicals
Mercuric Fixatives
Mercuric Fixatives:
Zenker’s
Zenker-Formol (Helly’s Sol’n)
Heidenhain’s Susa Fixtives
Schaudinn’s sublimated alcohol
B5 fixative
Best for fixation of blood forming or blood containing tissues i.e. pituitary gland, bone marrow, spleen, and liver
Zenker-Formol (Helly’s Solution)
Recommended for fixing small pieces of liver, spleen, connective tissue fibers and nuclei
Zenker’s fluid
Viral inclusions (Negri bodies)
Zenker’s Fluid
Recommended for tumor biopsies esp. of the skin
Heidenhain’s Susa Solution
Commonly used for Bone marrow samples
B-5 Fixatives
Principles of this leads to disruption of the internal salt linkages of the protein increasing the reactive basic groups and thereby enhancing acidophilic in staining
Chromate fixatives
Chromate fixatives:
Chromic acid
Potassium dichromate
Regaud’s (Moller’s fluid)
Orth’s fluid
Preserves carbohydrates, precipitates proteins
Chromic acid
Preserves mitochondria
Potassium dichromate
used for mitochondria, mitotic figures and golgi bodies
Regaud’s (Moller’s fluid)
demonstrates ricketssiae, tissue necrosis
Orth’s fluid
Mainly used for mucopolysaccharides
Lead fixatives
Picric Acid fixatives:
Bouin’s fluid
Brasil’s alcoholic - picro formol fixative
For fixation of embryos and pituitary biopsies
Bouin’s fluid
Rapidly denatures proteins by destroying hydrogen bonds
Alcoholic fixatives
Alcoholic Fixatives:
Methyl alcohol
Ethyl alcohol
Isopropyl alcohol
Carnoy’s fluid
Newcomer’s fluid
