food tests/enzymes

Question 1

where is amylase produced
what does it break down&what into

Answer 1

salivary glands, pancreas, small intestine
starch -> glucose

Question 2

where is protease produced
what does it break down&what into

Answer 2

stomach, pancreas, small intestine
proteins -> amino acids

Question 3

where is lipase produced
what does it break down&what into

Answer 3

pacreas, small intestine
lipids -> fatty acids+glycerol

Question 4

test for starch, positive result

Answer 4

iodine
orange/brown - blue/black

Question 5

test for sugar

Answer 5

benedict’s solution
light blue -green/brick red

Question 6

Describe test for sugars method

Answer 6

1.set up water bath using bunsen burner (water in beaker)
2.add food sample to test tube with a few drops of benedict’s solution
3.put test tube in water bath at 80 degrees for 5 min and note down colour changes

Question 7

Describe test for starch

Answer 7

1.add food sample to test tube
2. add few drops of iodine
3.note down colour changes

Question 8

Describe emulsion test for lipids

Answer 8

1.add food sample to a test tube
2. add few drops of distilled water and a few drops of ethanol
3.shake the solution gently and note down what u see

Question 9

Test for lipids
positive result

Answer 9

ethanol test
colourless-cloudy emulsion

Question 10

test for proteins
positive result

Answer 10

biuret solution
blue-lilac/purple

Question 11

Describe test for protein

Answer 11

1.add food sample to test tube
2.add few drops of siuret A and biuret B
3.shake gently and note down colour changes

Question 12

hazards in test for protein

Answer 12

biuret solution contains copper(II) sulfate which is dangerous if gets in eyes so wear goggles

biuret contains sodium hydroxide which is corrosive so if gets onto skin, wash immediately

Question 13

hazards in test for fats

Answer 13

ethanol is highly flammable, keep away from bunsen burners

Question 14

hazard in test for sugars

Answer 14

bunsen burner