Food, water + microbiology Flashcards
(49 cards)
Sewage treatment steps
- Raw sewage passed through screens
- Clarifying ponds
- Raw sewage removed
- Effluent put in aeration tanks
- Bacteria used to break down excreta
Natural processing
Waste separated by evaporation + rainfall
Clean water collected in aquifers
Disinfection of bottled water
- Ozone exposure to kill bacteria
- Filtration to remove particulate
- Reverse osmosis to remove naturally occurring salts + minerals
Indicator organisms - water
Campylobacter
Norovirus
Crytosporidium
Bacterial waterborne diseases
Vibrio cholerae
E.coli
Viral waterbourne diseases
Norovirus
Rotovirus
Protozoan waterborne diseases
Cryptosporidium parvum
Giardia
Norovirus
Non-enveloped
Icosahedral
Undergoes antigenic variation
Mutations increase transmission
Contagious - sheds viral particles
Endemic but seasonally epidemic in winter
Cryptosporidium types
C.parvum
C.homonins
Cryptosporidium
Risk factor of young + immunocompromised
Spread via oocytes
Caught at zoos + pools
Seasonal
Cryptosporidium lifecycle
- Excystation - oocyte breaks open in host gut
- Adhesion/locomotion
- Invasion
- Intracellular multiplication/survival
Intrinsic food preservation
Using factors already present
Intrinsic preservation examples
Water activity
pH
Antimicrobials
Competitive microbes
Extrinsic food preservation
Control of external factors
Extrinsic preservation examples
Heat
Low temperature
Modified atmosphere packaging
Radiation
Salmonella spp
Sugar -> lactic acid
Contains invasion proteins + LPS
Found in bird intestinal tract
Gastroenteritis - salmonella
Moderate
Gut infection
Typhoid fever - salmonella
Fatal
Infection of several organs
Campylobacter jejuni
In GI tract of red meats + poultry
Colonisation enzymes, adherence/invasion factors, flagellum, toxin (Cdt)
Listeria monocytogenes
Gram + rod
Resistant
Low incidence, high mortality
Biofilm, haemolytic, adhesion/invasion factors, flagella
Non invasive L.monocytogenes
GI tract infection
Invasive L.monocytogenes
Infection of blood, CNS and brain
Elderly + pregnant risk groups
E.coli
LPS, flagella, shin like toxin
Elderly + newborn risk groups
Enterohaemhorragic E.coli
Colon infection
Extreme danger