Gas Production chpt 2 Flashcards

1
Q

Is gas production acceptable?

A

Depends, acceptable by yeast for beer.

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2
Q

Blowing of bacuum packaged beef in refrigerator temp.. What am I?

A

Strict anarobe, psychrophile < 20C, survives antimicrobial treatments used in meat processing.
Fat not often used by microorganisms
ANSWER:
Clostridium spp. They have spores that can survive treatment and survive in cold temp.

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3
Q

Blown pack spoilage of modified atmosphere packaged sandwiches…what am I?

A

Has CHO, low oxygen, high CO2,

ANSWER: LAB

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4
Q

What corrective measures are done once determine source of organism?

A

Sampling, determine control measures ( ie Increase sanitizer concentration to kill LAB)

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5
Q

What does total count tell us about a food?

A
Count cells (direct) Tells us spoilage.  viable cells, and metabolic function. NOT anything about SAFETY. not diversity of microorganism. 
Changes in # in processing plant= something gone wrong. 
How old the food is, highly fermented= high total count.
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6
Q

TOtal counts PClate Count Agar (PCA) What is it?

A

General, support growth of many organisms.

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7
Q

What is the difference between rich and defined media?

A

Defined media is specific and only certain microorganisms grow on it. Growth inhibitor. While rich media has many microorganisms that can grow on it.

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8
Q

Temperature Effects growth..explain

A

Psychrotrophic vs mesophilic cold vs warm loving.

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9
Q

Pour vs. Spread plate Enumeration

A

POUR PLATE:
1 ml of sample put into sterile petri dish ( must be liquid), add sterile medium agar and mix gently.
SPREAD PLATE: 0.1ml of sample put onto prepoured agar plate, spread sample.
GOOD to have at least 30 colonies.

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10
Q

What is spoilage?

A

Not disease specific. simple.

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11
Q

Enrichment analysis, what does it tell us?

A

specific microorganism, but not numbers

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