Genetic Analysis

Question 1

The “genetic “ approach

Answer 1

look for mutants in which the process is defective. If we can make sense of mutant maybe learn how the process works

Question 2

Spontaneous mutants

Answer 2

uncorrected errors in DNA replication, natural mutagens ( x-rays, gamma rays, UV light)

Question 3

Induced Mutants

Answer 3

expose population to mutagen

Question 4

Mutagenesis

Answer 4

expose a population to a mutagen

Question 5

Mutant Screen

Answer 5

Look for mutants that have phenotype affecting the process of interest

Question 6

Steps after mutant is found

Answer 6

-Are the mutations dominant or recessive?
-Characterize the phenotype of each mutant
-How many genes have you identified?
-take all the mutants and perform complementation tests to determine how many genes you have identified
-Name your mutants and the genes they identify
-Determine which exact gene in the genome has been mutated

Question 7

Homologous Gene

Answer 7

a gene present in 2 species because of descent from common ancestor that preforms same function
-The same genes are present in yeast and humans doing the same function

Question 8

Model System Approach

Answer 8

studying a simpler species can yield results relevant to more complex species

Question 9

What do each of the A,B,C genes produce

Answer 9

A= sepal
A+B= petal
B+C= stamen
C= carpel