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Flashcards in Genetic engineering Deck (10)
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1

Definition of Inbred

Strain established by a minimum of 20 consecutivegenerations of sister × brother matings (sib matings)

2

Definition of Outbred

Stock/line established and maintained by strictavoidance of sib mating

3

F1 hybrid

Created by mating mice of two inbred strains in adefined direction

4

F2 hybrid

Created by mating two F1 hybrid mice

5

Recombinantinbred

Lines established by inbreeding of the progeny ofindividual F2 hybrid sibling pairs for at least 20generations

6

Congenic

Introduction of a mutation onto a defined inbredstrain background by 5–10 or more generations ofmatings back to the inbred strain with selection forthe mutation (backcrossing)

7

Consomic

An inbred strain that carries one chromosome fromanother inbred strainTen generations of backcrossing and selection for thedonor chromosome is required for a fully consomicstrain

8

Conplastic

An inbred strain that carries the nuclear genome ofone inbred strain and the cytoplasm and cytoplasmic(mitochondrial) genome of anotherSince the egg contributes the embryonic cytoplasm,10 generations of backcrossing inbred females to aninbred male recipient is required for a fully conplasticstrain

9

RFLP

Restriction fragment length polymorphism - DNA is isolated, digested by bacterial restriction enzymes, then the lengths of the digested fragments compared for differences.

10

SNPs

Single Nucleotide Polymorphisms - identified variations in single nucleotides used to monitor genotypes