Genetic infomation, variation and relationships between organisms Flashcards
(58 cards)
What are the two key similarities between eukaryotic and prokaryotic DNA?
Both are made up of DNA nucleotides and are joined together by phosphodiester bonds
The nucleotides contain deoxyribose, a phosphate group, and a nitrogenous base.
What are the three key differences between eukaryotic and prokaryotic DNA?
- Eukaryotic DNA is longer
- Eukaryotic DNA is linear
- Eukaryotic DNA is associated with histones, while prokaryotic DNA is not
Eukaryotic DNA occurs as straight lines in chromosomes, while prokaryotic DNA forms circular loops.
What is the structure of DNA in eukaryotic cells?
DNA is tightly coiled into chromosomes within the nucleus, and forms nucleosomes by wrapping around histone proteins
This organization allows DNA to fit within the nucleus.
How does the DNA in mitochondria and chloroplasts compare to prokaryotic DNA?
Both have short sequences of circular DNA that are not wrapped around histone proteins
They have their own DNA to transcribe and translate enzymes necessary for photosynthesis (chloroplasts) and respiration (mitochondria).
Define a gene.
A sequence of DNA that codes for the amino acid sequence of a polypeptide and functional RNA
It also codes for an mRNA molecule.
What is a locus?
The exact position of a particular gene on a chromosome
‘Locus’ can be remembered as ‘location’.
What is a triplet in the context of DNA?
A sequence of three bases on DNA that codes for a particular amino acid
Triplets are essential for understanding the genetic code.
List the three main features of the genetic code.
- Degenerate
- Universal
- Non-overlapping
The degenerate feature means multiple triplets can code for the same amino acid.
Explain what is meant by the genetic code being degenerate.
There are more than one triplet of bases that codes for the same amino acid
For example, glycine can be coded by GGG, GGA, GGC, and GGU.
What does it mean for the genetic code to be universal?
The same triplet of bases codes for the same amino acid in all organisms
This consistency is important for the evolutionary connection among species.
What does non-overlapping mean in the genetic code?
Each base is only involved in one triplet, and codons are read as discrete units
This minimizes the impact of mutations.
Differentiate between introns and exons.
- Introns: Sequences of DNA bases that do not code for polypeptides
- Exons: Sequences of DNA bases that do code for amino acids
Exons are the coding regions of a gene.
What is a codon?
Three bases on mRNA that code for a specific amino acid
The start codon initiates translation, while stop codons signal the end.
Define a genome.
An organism’s complete set of genes in a cell
This includes all genetic material of the organism.
What is a proteome?
The full range of proteins that a cell is able to produce
The proteome can change depending on the proteins needed by the cell.
How does the genome of bacteria compare to that of humans?
Bacteria have an average of 600,000 DNA base pairs, while humans have about three billion
This illustrates the vast differences in genomic complexity.
What is messenger RNA (mRNA)?
A short, single-stranded copy of one gene, found in both the cytoplasm and nucleus
It is created during transcription.
What is the role of transfer RNA (tRNA)?
tRNA carries specific amino acids to the ribosome during translation
It has an anticodon that is complementary to the mRNA codon.
Explain the process of transcription.
A complementary mRNA copy of one gene is created from DNA in the nucleus
Key steps include unwinding DNA, aligning RNA nucleotides, and splicing out introns.
What happens during translation?
mRNA binds with a ribosome, and tRNA brings amino acids to form a polypeptide chain
This process continues until a stop codon is reached.
What is the role of the Golgi body in protein synthesis?
It modifies the polypeptide chain into its functional structure
This includes folding into secondary, tertiary, or quaternary structures.
What is a gene mutation?
A change in the base sequence of DNA that occurs randomly during DNA replication
Mutations can be influenced by exposure to mutagenic agents.
What is a gene mutation?
A change in the base sequence of DNA that occurs randomly during DNA replication.
What are mutagenic agents?
Agents that can interfere with DNA replication, increasing the likelihood of mutations. Examples include:
* High energy radiation (UV light, gamma rays, x-rays)
* Chemicals (carcinogens like mustard gas and cigarette smoke)