Genetic Modification Flashcards
What is natural selection?
The influence of the environment on survival and/or reproduction so that organisms with some characteristics are more successful at producing offspring than others
Process of natural selection
- environment creates a selection pressure
- animals with features (adaptations) which are best suited for this environment will survive and breed
- they pass on genes to the next generation
Rabbit example
Snowy environment Two colours of rabbit: brown and white White rabbits are more camouflaged and better adapted Predators eat brown rabbits White rabbits survive Breed Pass on genes Over a long period of time, more rabbits are white and evolution can occur
Peppered moth example
- pollution causes lots of smog in forests, covering the trees and creates a section pressure
- mutation causes a dark moth to be produced
- the forest started with an equal number of white moths and black moths
- black moths are better adapted as they are more camouflaged
- the predators ate the white moths as they were easier to see
- black moths are more able to survive
- black moths breed and pass on genes to n cy generation
Process of natural selection ( long version)
- individuals and species produce genetically diverse offspring
- those individuals offspring compete for external things such as sunlight, Good, water and mates, whilst attempting to avoid predators
- offspring with many competitive traits survive and reproduce
- the less competitive die and reproduce less
- the adapted raw frequency increases in population
What is the intifada cause of natural selection in an organism?
Mutation- caused by the selection pressure (changes in the environment)
Antibiotic resistance
- there is a normal population of bacteria
- a mutation occurs, which causes the bacteria to develop antibiotic resistance.
- Antibiotics are applied in attempt to kill the bacteria
- those who are not resistance to the antibiotics are destroyed and die. Those who are resistant are at an advantage.
- the number of non- restraint bacteria decreases as more and more die out
- the bacteria with antibiotic resistance breed and pass on their genes to the next generation
- resistant population increase in number over many generations
If you were a cow farmer, what qualities would u be looking for?
- calm, good producer of milk, good health, healthy diet, food appetite, not susceptible in infection, genes for good milk production
Example of real life genetic modification
Sheep predicting spider silk
Featherless chicken
Glow in the dark animal
Golden rice- vitamin A
Lack of insulin means
You are diabetic- you can’t regulate blood glucose levels
Stages of genetic engineering
1- (insulin) gene cut using restriction enzymes (complimentary sticky ends)
2- vector (plasmid/virus) cut with same restriction enzyme
3- DNA ligase enzyme added to seal overhang making recombiant DNA which is introduced into bacterial cell
4- recombiant DNA molecules replicate and cells divide
5- grow inside a fermenter (optimum temp, PH and glucose)
Ligase
Enzyme used to join sections of DNA with complimentary sticky ends together via S-P backbone
Vector
A structure used to transfer generic material from one organism to another
E.g. plasmid/ bacteriophage
Plasmid
A short loop of circular DNA found in a bacteria. Used In genetic engineering to restrict endonuclease and is where the gene insulin sticks to
How can large volumes of insulin be produced once the vector has taken up volumes of the insulin gene into the bacteria?
- bacteria reproduce inside a fermenter
