Genetics

Question 1

Explain gene cloning (6 steps)

Answer 1

1. DNA purified to be cloned
2. DNA cut by restriction endonucleases
3. Target DNA fragments inserted into plasmid vector ~ recombinant DNA
4. Transport into living cells which multiply fast
5. Growth encouraged to replicate original DNA
6. Recombinant cells with gene of interest identified

Question 2

Explain the RPE-65 gene

Answer 2

essential for vision ~ converts VitA to be usable
mutation causes blindness at birth