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Flashcards in Gram(-)Bacteria Deck (28)
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1

What are 2 clinically significant genera for gram (-) cocci?

1. oxidase-postive
2. Gram (-) diplococci

2

What are 3 major Gram (-) cocci?

Neisseria (N. gonorrhoeae, N. meningitidis) Moraxella (M. catharralis)

3

What are the growth characterisitics for Gram (-) cocci?

fastidious bacteria (prefer supplemental media)

4

Nesseria spp. may be found colonizing most ______.

mucous surfaces

5

Nesseria menigitiidis is a well recognized cause of _______ and/or ________

fulminant septic shock and/or meningitis

6

Moraxella catharralis causes upper and lower ______, _____, and rarely _______ or _________.

respiratory tract infections; otitis media; bacteremia or endocarditis.

7

What is lactose fermentor on MacConkey's test and Indole positive?

E-coli

8

What is lactose fermentor on MacConkey's test and Indole negative?

Klesbsiella, Enterobacter, Citrobacter (large, mucoid colonies)

9

What is non-lactose fermentor on MacConkey's test and oxidase positive?

pseudomonas aeruginosa (metallic colonies, grape-like odor)

10

What is non-lactose fermentor on MacConkey's test and oxidase negative and swarming (blood agar)?

proteus mirabilis

11

What is non-lactose fermentor on MacConkey's test and oxidase negative and not swarming (blood agar)?

Enterobacteracae:
Salmonella (motile, H2S)
Shigella (non-motile)
Serrtatia (may produce pink/red pigment)
Other non-enterobeacteriacae:
acinetobacter
burkolderia

12

What are the most common Gram (-) organisms?

enterobacteralies (enterobacteriacae)

13

Other non-fermenting gram (-) rods, generally environmental organism that cause ______ infections. Name these bacteria.

Opportunistic
pseudomonas aeruginosa
acinetobacter species
burkolderia cepacia

14

What do plate looks like for pseudomonas aeruginosa?

non-lactose fermenting, gram (-) rod, oxidase +, metallic sheen and grape-like odor in culture

15

What is non-lactose fermenting, oxidase - and gram (-) rods?

Acinetobacter spp
Burkolderia spp

16

______ is an opportunistic infection, often ______, may be particularly ______ _______

A. baumanii; nosocomial; drug resistant

17

_______ is an important pathogen in CF patients.

Burkolderia spp

18

Haemophilus influenzae uses what media type and causes what infections?

Media: chocolate agar
Infections: meningitis, pneumonia

19

Legionella uses what media type and causes what infections?

Media: buffered charcoal yeast extract (BCYE) -L-cystine
Infection: pneumonia

20

Bordetalla uses what media type and causes what infections?

Media: Bordet-Gengou
Infection: whopping cough (pertussis)

21

HACEK uses what media type and causes what infections?

Media: Chocolate agar
Infection: endocarditis--poor dentition

22

What infection does kingella cause in pediatric patients?

septic arthritis

23

What does HACEK stand for?

H: Haemophilus species
A: aggregatibacter actinomycetemocomitans
C: Cardiobacterium hominis
E: Eikenella corrodens
K: Kingella
Commonly colonize the human oropharynx and normal indigenous flora

24

Pasturella uses what media type and causes what infections?

Media: Chocolate agar
Infection: infectious associated with animal bites

25

Bacteroides and Fusobacterium uses what media type and causes what infections?

Media: anaerobic organsim (no O2)
Infection: usually endogenous cause by oral flora or gut flora disruption.

26

Francisella tularensis (tularemia) uses what media type and causes what infections?

Media: chocolate agar
infection: tick and deer fly bits, rabbits

27

Brucella spp uses what media type and causes what infections?

Media: chocolate agar, blood agar (poorly)
Infection: unpasteurized dairy products, contact with animals.

28

Yersinia pestis uses what media type and causes what infections?

Media: Blood agar (slowly), MacConkey agar
Infection: bite from infected fleas, inhalation fo aerosolized particles (Plague)