Gram Negative Cocci Flashcards
(103 cards)
0
Q
Neiserria have an optimal growth in
A
Moist temp
1
Q
Obligate aerobic
Non motile
Non hemolytic
A
Neisseria
2
Q
How many percent of CO2 does neisseria have
A
2-8%
3
Q
Carbohydrate fermenters of neiserria
A
Glucose
Maltose
4
Q
Most of the colonies are pigmented exccept
A
N.flava
N.flavescens
N.subflava
5
Q
Where is the Direct inoculation of specimens
A
At the bedside
6
Q
Neiserria are sensitive to
A
Drying
Extremes of temp
7
Q
Microscopy of neisseria
A
Gram - diplococci
With coffee or kidney bean shaped
8
Q
Major pathogens in neiserria
A
N.gonorrheae
N.meningitidis
9
Q
Biochemical tests in neiserria
A
+ oxidase
+ catalase
10
Q
Never considered part of the normal flora
A
N.gonorrheae
11
Q
Only known host in n.gonorrheae
A
Man
12
Q
N.gonorrheae is found in
A
Urogenital tract
Anorectal area
Oropharynx
Conjunctiva
13
Q
Mode of transmission of n.gonorrheae
A
Sexual contact
14
Q
N.gonorrheae produce an
A
IgA protease
15
Q
Antibody found in mucous membrane secretions
A
IgA
16
Q
Culture in n.gonorrheae
A
CAP
17
Q
Pvf of n.gonorrheae
A
Common pili
18
Q
Colonial types
A
T1 & T2 (virulent)
T3 & T5 (avirulent)
19
Q
Means of flow of seed and brothel
A
Gonorrheae
20
Q
Incubation period of gonorrheae
A
2-7 days
21
Q
Symptoms of gonorrheae
A
Purulent discharge
Lower abdominal pain
Dysuria (men)
Dysuria and vaginal bleeding (women)
22
Q
Untreated gonococcal cervicitis may cause what
A
Sterility
Perihepatitis
23
Q
It is a syndrome where untreated gonococcal cervicitis may cause sterility and perihepatitis
A
Fitz-hugh-curtis syndrome
24
Infection where there is rectal pain and bloody stools
Anorectal infections
25
Gonococcal eye infection during vaginal delivery through an infected birth canal
Conjunctivitis
26
Other term for conjunctivitis
Ophthalmia noenatorum
27
Most important factor in the lab diagnosis of n.gonorrhea
Specimen collection and handling
28
Specimens in n.gonorrheae
```
Pus and secretions from urethra
Cervix
Prostate
Rectal mucosa
Throat
Joint fluid
```
29
Used for collection of the specimen
Dacron or rayon swabs
30
This swab should be avoided due to the presence of toxic fatty acids in the cotton fibers
Cotton swab
31
Non gonococcal urethritis
C.trachomatis
32
Smears for gs should be prepared from these specimens
Urogenital specimens
33
Cultures for confirmation
Bap
| Cap
34
Specimens on swab should be prepared onto media in what pattern
Z pattern
And
Cross streaked with a loop
35
Agar with all the TMA components + trimethoprim lactate
Modified thayer martin agar
36
Agar where vancomycin conc. is increased
Martin lewis medium
37
Chocolate agar with an enrichment supplement and antibiotics
Thayer martin agar
38
A transparent medium with lysed horse blood, horse plasma, and yeast dialysate
Nyc medium
39
Antimicrobial agents in nyc medium
Vancomycin
Colistin
Trimethoprim
Amphotericin B
40
Genital mycoplasma
Mycoplasma hominis
| Ureaplasma urealyticum
41
Examples of transport medium
Cary blair
Amies
Jembec
Transgrow
42
Required temp for incubation of agar plates
35-37 degree celcius
43
Incubation period for agar plates
72 hours
44
Standard method of identifying n.gonorrheae
CHO utilization test
45
Medium used in cho urilization test
CTA (cystine trypticase agar)
46
Composition of cta
1% carbohydrate
| Phenol red
47
+ result in cho utilization test
Yellow color
| within 24-72 hrs at 35 degree celcius
48
Rgt in oxidase test
1% tetramethyl-p-phenylenediamine-dihydrochloride
49
+ result in oxidase test
Purple color within 10 seconds
50
Other + oxidase bacteria
N.cinerea
N.meningitidis
M.catarrhalis
51
Rgt used in superoxol test
20-30% H2O2
52
+ result in superoxol test
Vigourous bubbling
53
+ in dnase test
| - in dnase test
M.catarrhalis +
| N.gonorrheae -
54
Highly specific and sensitive test
Fat
Or
Fluorescent antibody test
55
Test that confirms the morphologic appearance of the bacteria
Fat
56
Confirms the biochemicql identification of n.gonorrheae
Coagglutination
57
+ result of coagglutination
Agglutination
58
Nucleic acid amplification
Molecular assays
59
Specimen for molecular assay
Urethral swabs
60
Blood protein
Complement
61
Used for culture confirmation or direct detection in endocervical or urethral swab specimens
Chemiluminescent nucleic acid probe
62
Causative agent of epidemic meningococcal meningitis/meningococcemia/cerebrospinal fever/spotted fever
N.meningitidis
63
N.meningitidis is the leading cause of
Fatal bacterial meningitis
64
May be found as a commensal inhabitant of the upper respiratory tract of the carriers
N.meningitis
65
What kind of fermenters are n.meningitidis
Glucose and maltose fermenters
66
Microscopy of n.meningitidis
Encapsulated strains with halo around organism
67
Serogroups of n.meningitidis
```
A
B
C
Y
W-135
```
68
Virulence factors of n.meningitidis
Pili
Polysaccharide capsule
IgA1
69
Coverings of the brain
Meninges
70
Refers to the presence of n.meningitidis in the blood
Meningococcemia
71
Source of epidemics in meningococcemia
Oral secretions
| Respiratory droplets
72
Signs and symptoms of meningococcemia
Frontal headache
Stiff neck
Fever
73
They are develop because of endotoxins
Petechial skin lesions
74
Syndrome where there is production of hemorrhage in the adrenals
Waterhouse-friderichsen syndrome
75
Specimen in n.meningitidis
Csf
Blood
Nasopharyngeal swab
Petechial skin lesions
76
N. Meningitidis is senstive to
SPS
77
Process of concentarting csf fluid
Cytocentrifugation
78
When is the highest yield of positive of csf gram stain is obtained
When specimens are concentrated
79
Culture media in n.meningitidis
Bap
Cap
Tma
80
This resembles n.meningitidis on gonococcal selective media
N.lactamica
81
Orher term for beta lactamase test
Cephalosporinase test
82
Rgt used in beta lactamase test
Cephalosporin disk
83
+ result in beta lactamase test
Deep pink
Or
Red color
84
Other name of moraxella catarrhalis
Branhamella catarrhalis
85
How does m.catarrhalis resembles neiserria
By exhibiting gram - coccal morphology
86
Mos commonly isolatmemberof the genus moraxella
M.catarrhalis
87
Location of m.catarrhalis
Oropharynx
88
M.catrrahlis are only isolated from
Humans
89
3rd most common cause of otitis media and sinusitis in children
M.catarrhalis
90
Culture in m.catarrhalis
Honeypuck appearance
91
Biochemical tests in m.catarrhalis
+ oxidase
| + catalase
92
Does not ferment any sugar
Asaccharolytic
93
Cause of otitis media
M.catarrhalis
94
Specimen in butyrate esterase test
Ear culture
95
+ result in butyrate esterase test in m.catarrhalis
Blue color
96
Protein in blood
Transferrin
97
Organ of attachment
Common pili
98
Complication of PID
Fitz hugh curtis syndrome
99
Prevent swarming
Trimethoprim lactate
100
Inhibits gram -
Colistin
101
Inhibits gram +
Vancomycin
102
Screening test
Molecular assay
