Staphylococci are facultatively anaerobic except this
S.saccharolyticus
They are catalase producing bacteria and facultatively anaerobic
Staphylococci
Buttery looking
S.aureus
Tube medium used to know if its motile or not
SIM
sulfide indole motility
Old stocking odor
MSA
Designed to s.aureus
MSA
Most virulent species
S.aureus
PVF of s.aureus
Coagulase
Culture in s.aureus
Golden yellow pigment (lipochrome)
Mild inflammation of hair follicle
Folliculitis
Large, superficial abscesses
Furuncles
Develop from multiple furuncles
Carbuncles
Crusty lesions
Impetigo
Has the ability to produce the clumping factor
S.aureus
Other term for clumping factor
Cell bound coagulase
Bound to the cell wall
Clumping factor
Not bound to cell wall
Free coagulase
Spreading factor enzyme
Hyaluronidase
Fat splitting enzyme
Lipase
It allows aureus to move to move in thick fluids
DNAse
Lowers viscosity of exudates
Dnase
Breaks down penicillin
Beta lactamase
Food poisoning
Enterotoxin A
Never food poisoning
Enterotoxin F
This toxin attacks and kills WBCs
Leukocidin
Causes anemia
Hemolysin
Destroys rbc, platelets, macrophages
Alpha hemolysin
Reagent used for CAMP test for group of streptococci
Beta hemolysin
Destroys spingomyelin and rbc around nerves
Hot-cold lysin
Beta hemolysin
TSS
Toxic shock syndrome
Natural anticoagulant
Heparin
Reagent in coagulase test
Rabbit plasma
This method detects clumping factor
Slide method
This method detects free coagulase
Tube method
+ result in coagulase test
Clot
Slide coagulase + organisms
S.aureus
S.lugdunensis
S.schleiferi
Tube coagulase + organisms
S.aureus
S.hyicus
S.intermedius
Used to differentiate pathogenic from nonpathogenic staphylococci
Maniitol fermentation test
Ph indicator in mannitol fermentation test
Phenol red
End product in mannitol fermentation test
Acid production
Differentiates s.aureus from s.intermedius
Voges proskauer test
Reagents used in VP test
Alpha naphthol
Potassium hydroxide
+ result in VP test
Acetoin
Super bug
MRSA
MRSA
Methicillin resistant s.aureus
+ result in chromogenic test
Mauve colored colonies
Any change in the color of the medium
This gene results in resistance to erythromycin but susceptible to clindamycin
Msr A gene
Normal flora of the skin
Staphylococcus epidermidis
Associated with community acquired UTI
S.saphrophyticus
Selective medium for gram + cocci
Pea agar
Phenyl ethyl alcohol
Reagent used in catalase test
3% hydrogen peroxide (H2O2)
+ result catalase test
Bubble formation
Enzyme of blood
Peroxidase
Catalase negative staphylococci
S.aureus subsp. anaerobius
S.saccharolyticus
This reagent produce bubbles
Hydrogen peroxide
This enzyme results to bubble formation
Peroxidase
Detects slide coagulase + staphylo
Pyrrolidonyl arylamidase test
+ result in pyrrolidonyl arylamidase test
S.lugdunensis
S.intermedius
S.schleiferi
- result in pyrrolidonyl arylamidase test
S.aureus
+ result in dnase test
Pink color= cell died
- result in dnase test
Blue color= cell alive
Detects tube coagulase + staphylococci
VP test
+ result in vp test
S.aureus
- result in vp test
S.intermedius
Test used to detect inducible clindamycin
Double disk diffusion test
+ result in d test
D shaped
Antibiotics in d test
Erythromycin
Clindamycin