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Microbiology 🦠🧫🔬 > Gram Negative Rod 1 > Flashcards

Gram Negative Rod 1 Flashcards

1
Q

Oxidase test of gram negative rod ?

A

Negative

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2
Q

The fermentation of which sugar is considered a differential characteristic?

A

Lactose

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3
Q

Gram negative bacilli grow well on which agar?

A

Mconky agar

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4
Q

Example of “coliform bacilli” and lactose fermentor ?

A

Escherichia
Klebsiella

live as
commensals in the intestine of man and
animals, and capable of producing diseases if
change their habita

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5
Q

 Proteus  Salmonella  Shigella  Yersinia
Are considered?

A

Non lactose fermintors gram negative bacili

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6
Q

Pseudomonas
aeruginosa and Bacteroides
species are Gram-negative bacilli, and
normally found in human intestines,
they are considered non-
Enterobacteriaceae because?

A

They dont share the general characteristics of Enterobacteriaceae
P.aeruginosa is an obligate aerobe
and oxidase positive, whereas
Bacteroides is a strict anaerobe.

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7
Q

Enterobacteraceae—Antigenic structure
Composed of three major groups of antigen

A

O : somatic antigen most external
H: motile
K: capsule

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8
Q

E .coli antigenic structure are ?

A

O157,H7

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9
Q

It is used as one of the indicators of
fecal pollution of water.

A

E.coli

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10
Q

Grows on MacConkey agar producing rose
pink colony due to lactose fermentation.

A

E.coli

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11
Q

Ferment glucose, lactose, maltose,
mannite, sucrose and salicin with
production of acid and gas.

A

E.coli

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12
Q

State the IMVC test of E.coli

A

++- -

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13
Q

Which starin of E.coli conatin K antigen?

A

Uropathogenic E. coli: causing urinary tract infection

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14
Q

common cause of neonatal meningitis.

A

E.coli K1

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15
Q

Commenest cause of hospital acqureid pneumonia?

A

E.coli

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16
Q

Major causing agent of travelers diarrhea?

A

Enterotoxogenic E.coli

17
Q

Heat labil and heat stable toxin are associated with ?

A

Enterotoxogenic E.coli

18
Q

Hemooragic E.coli toxin ?

A

Shiga like toxin

19
Q

most common strains producing disease in developed
countries.

A

Enterohemorrhagic E. coli (EHEC)

20
Q

strains are associated with hemorrhagic colitis and
hemolytic uremic syndrome (HUS). Serotype O157:H7 is
most commonly isolated.

A

Enterohemorrhagic E. coli (EHEC)

21
Q

What is detected in neonatal meningitis cause by E.coli ?

A

E. coli K1 antigen is detected by in CSF

22
Q

Direct smear of E.coli only useful in ?

A

Sterile specimen CSF

23
Q

In cases of E.coli Urine should be quantitatively cultured on?

A

Cled medium

24
Q

Culture of E.coli ?

A

Mconky agar and blood agar

25
Q

Commenest cause of hospital acquired infection in gram - ve ?

A

Multi-drug resistant Klebsiella strains

26
Q

All genome of E.coli are non capsulated except ?

A

E.coli that cause urinary tract infection

27
Q

Non-motile and usually capsulated Gram-
negative bacilli.

A

Klebseillea

28
Q

rose pink colonies are produced, usually
mucoid due to the production of abundant
extracellular slime.

A

Klebsiellea on mconky agar

29
Q

(IMVC) of K. pneumoniae is

A
      • +
30
Q

All bacteria have polysaccharides capsule except?

A

Anthrax , polypeptide

31
Q

K. ozaenae

A

Atropic rhinitis

32
Q

K. rhinoscleromatis

A

Rhinscleroma

33
Q

You’re a public health epidemiologist who is called to investigate an outbreak of bloody diarrhea in 16 people. You find that it is associated with eating rare hamburgers in a particular fast-food restaurant. A culture of the remaining uncooked hamburger grows a gram-negative rod that produces a dark purple colony on EMB agar, which is evidence that it ferments lactose. Which one of the following bacteria is the most likely cause of this outbreak?
(A) Escherichia coli
(B) Salmonella enterica
(C) Salmonella typhi
(D) Shigella dysenteriae

A

Escherichia coli

34
Q

Regarding the members of the family Enterobacteriaceae, which one of the following is the most accurate?
(A) All members of the family are anaerobic, which means they must be cultured in the absence of oxygen.
(B) All members of the family ferment lactose, which is an important diagnostic criterion in the clinical laboratory.
(C) All members of the family have endotoxin, an important pathogenetic factor.
(D) All members of the family produce an enterotoxin, which ADP-ribosylates a G protein in human enterocytes.

A

All members of the family have endotoxin, an important pathogenetic factor.

Microbiology 🦠🧫🔬 (9 decks)

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