growth practicals

Question 1

whats inoculating

Answer 1

bunsen flame, loop in hot til red for a second and cool for 20 seconds

Question 2

whats streak plating

Answer 2

lid face down, rub culture loop on small section and sterilise and cool loop. rotate plate and do cross stitch and wiggle in the middle to drag the colonies

Question 3

what colour is a lactose fermenter

Answer 3

red colonies

Question 4

what colour is non-latose fermeneters

Answer 4

pale yellow

Question 5

what colour is gram + after staining

Answer 5

purple/ black

Question 6

what colour is gram - after staining

Answer 6

pink

Question 7

what is enriched media

Answer 7

extra growth requirments

Question 8

what is differential media

Answer 8

preliminieary differentional of some organisms particulary the gut into 2 groups

Question 9

what is selective media

Answer 9

inhibits growth of commensal organisms e.g. MacConkeys

Question 10

what is binary fision

Answer 10

replication mechanism of bacteria at a rate of about 20 mins per generation

Question 11

what is alpha and beta haemlysis

Answer 11

alpha: partial: each colony is surrounded by a zone of incomplete haemolysis: green pigment and reduced product of haemoglobin e.g. strep pneumonia
beta: completes zone clear in which blood is completely lysed e.g. strep pyogenes

Question 12

what is blood agar

Answer 12

enriched and differential

Question 13

what is cytopathic effect

Answer 13

structural change in host cells due to viral invasion causing lysis of host cells