Harada Mori

Question 1

harada mori is for the detection of infections with

Answer 1

hookworm, strongyloides, trichostrongylus

Question 2

harada mori facilitates the specific identification of their larval stage:

Answer 2

rhabditiform larvae
filariform larvae

Question 3

fecal material to be culture should be ___

Answer 3

not refrigerated

Question 4

fecal material to be cultured in the harada mori should not be refrigerated because

Answer 4

some species will not develop

Question 5

what are the materials needed for harada mori procedures

Answer 5

fresh stool sample from human or animal
10 or 15 ml centrifuge tubes
filter paper strip (tapered at one end)
test tube rack
applicator stick
microscope
dropper or pipette
glass slides
coverslips (2x2)
forcep
aluminum foil (1” x 1”) or stopper
distilled or sterilized water

Question 6

steps for harada-mori procedure

Answer 6

1. label the test tube and place about 2ml of distilled or heat sterilized water
2. smear about HALF GRAM of feces (size of a marble) on the filter paper strip using a wooden applicator stick, leaving the margin at both sides and ends unsmeared
3. insert the filter paper strip inside the 10 or 15 ml centrifuge tube with one end touching the bottom of the tube. make sure that the water is slightly below the fecal mass.
4. cover the mouth of the tube with aluminum foil or rubber stopper and allow it to stand for 8 TO 10 DAYS in a DARK PLACE at ROOM TEMPERATURE
5. After the 5th day, the hookworm eggs will have
   developed into rhabditiform larvae. On the 10th
   day, it will develop into filariform larvae.
6. Immerse the tube in hot water (not boiling) for 15
   minutes to kill the larvae.
7. Remove the filter paper with a forcep and
   centrifuge the remaining liquid for 3 to 5 minutes.
8. Pipette and throw the supernatant. Transfer a drop
   of sediment into a glass slide and place a coverslip.
   Examine under the LPO for presence of larvae. To
   identify the species, examine under the HPO.

Question 7

how many days does it take for the hookworm egg to developed into rhabditiform

Answer 7

5 days

Question 8

how many days does it tale that the hookworm will developed into its filariform larvae stage

Answer 8

10 days

Question 9

how long does it take to immerse the tube in the hot water that is not boiling to kill the larvae

Answer 9

15 minutes

Question 10

the remaining liquid will be centrifuges for how many minutes

Answer 10

3 to 5 minutes

Question 11

using the LPO is for

Answer 11

presence of larvae

Question 12

using the HPO

Answer 12

identify the species `

Question 13

it is short duldous esophagus

Answer 13

rhabditiform

Question 14

long esophagus and closes mouth

Answer 14

filariform larvae

Question 15

the tail end is notched

Answer 15

strongyloides

Question 16

the tail is pointed

Answer 16

hookworm

Question 17

tail is sharp

Answer 17

necator

Question 18

tail is blunt

Answer 18

ancylostoma

Question 19

the difference between the rhabditiform of strongyloides stercoralis and hookworm

Answer 19

genital primordium