Non-pathogenic Intestinal Amoeba

Question 1

What are the non-pathogenic intestinal amoeba (8)

Answer 1

1. Entamoeba coli
2. Entamoeba dispar
3. Entamoeba hartmanni
4. Entamoeba gingivalis
5. Entamoeba polecki
6. Iodomoeba butschlii
7. Endolimax nana
8. Blastocystis hominis

Question 2

The only pathogenic intestinal amoeba

Answer 2

Entamoeba histolytica

Question 3

Entamoeba histolytica can be found in

Answer 3

Colon

Question 4

Who discovered the Entamoeba histolytica in 1873 and named it Amoeba coli?

Answer 4

Dr. Fedor A. Lösch

Question 5

Who discovered the Entamoeba histolytica in 1873 and named it Amoeba coli?

Answer 5

Dr. Fedor A. Lösch

Question 6

Amoeba coli then was changed into Entamoeba histolytica by?

Answer 6

Fritz Schaudinn

Question 7

The controversy between the species of Entamoeba histolytica was over:

Answer 7

● different size
● pathogenicity
● isoenzyme typing
● molecular techniques

Question 8

It is alo known as the “small-raced E. histolytica”

Answer 8

Entamoeba hartmanni

Question 9

He proposed that there are 2 types of Entamoeba histolytica (one pathogenic and one non-pathogenic)

Answer 9

Emile Brumpt

Question 10

This technique allowed scientists to distinguish between pathogenic and non-pathogenic E. hisyolytica

Answer 10

Ppolymerase Chain Reaction - DNA and biogenetics

Question 11

They named the E. histolytica into two separate species

Answer 11

Diamond and Clarke

Question 12

What are the two type of Entamoeba that Clarke and Diamond named

Answer 12

E. histolytica - pathogenic
E. dispar - nonpathogenic

Question 13

This is morphologically identical with Entamoeba hisyolytica but genetically different (DNA and ribosomal RNA)

Answer 13

Entamoeba dispar

Question 14

This is morphologically identical with Entamoeba hisyolytica but genetically different (DNA and ribosomal RNA)

Answer 14

Entamoeba dispar

Question 15

The only difference between Entamoeba dispar and Entamoeba histolytica is:

Answer 15

one base pair

Question 16

Number of base pairs of E. histolytica

Answer 16

100

Question 17

Number of base pairs of E. dispar

Answer 17

101

Question 18

Cytoplasm of the E. dispar trophozoite

Answer 18

fine granular

Question 19

What are the inclusions of the E. dispar trophozoites

Answer 19

1. Bacteria
2. NEVER contains RBC
3. Bacteria or Debris in vacoules

Question 20

The number of nucleus of E. dispar trophozoite

Answer 20

1 only

Question 21

Describe the nucleus of the E. dispar trophozoite

Answer 21

● small and single with evenly distributed peripheral chromatin

Question 22

Describe the karyosome of the E. dispar trophozoite

Answer 22

Small and Centrally located

Question 23

What is the movememt of the E. dispar trophozoite

Answer 23

progressive and purposeful or directional

Question 24

the only intestinal amoeba that is purposive (unidirectional)

Answer 24

E. dispar trophozoite

Question 25

■ capable of endocytosis (ingestion orengulfment of substances or molecules) and exocytosis (excretion of substances; canalso be done via the plasma membrane)

Answer 25

E. dispar trophozoite

Question 26

■ attachment to substrate ■ penetration through tissue (can lead toextraintestinal amoebiasis - liver - mostcommon, lungs, brain) ■ release of cytotoxic substances ■ contact cytolysis of pus cells - where theamoeba will attach itself to penetrate the tissues

Answer 26

E. dispar trophozoite

Question 27

Inclusions for E. dispar cysts

Answer 27

Inclusions are usually observed in the YOUNG and IMMATURE CYSTS

Question 28

how many nuclei does the mature have and immature have of E. dispar cysts

Answer 28

Mature - 4 nuclei Immature - less than 4 nuclei

Question 29

Ingested mature cyst of E.dispar can cause

Answer 29

Infection of E.histolytica or dispar

Question 30

What can be tha cause/s of ingestion of cyst of E. dispar

Answer 30

● contaminated food ● contaminated water ● mechanical vectors (mosquieto and flies)

Question 31

The infection of E. dispar/histolytica can cause two types of amebiasis namely:

Answer 31

1. Intestinal Amebiasis 2. Amebiasis characterized by dysentry

Question 32

True or false: E. dispar cyst are resistant to acid pH

Answer 32

TRUE

Question 33

E.dispar cyst can survive for how many days at a certain temperature

Answer 33

● 2 days at 37° C ● 60 days at lower temperatures

Question 34

E.dispar cysts are viable on:

Answer 34

● stool samples ● soil ● freshwater ● seawater ○ a week at 37°C ○ a month at 20°C

Question 35

E.dispar chromatoidal bars:

Answer 35

present with rounded ends in young cysts

Question 36

E.dispar cyst nucleus

Answer 36

1-4 nuclei

Question 37

E.dispar cysts glycogen

Answer 37

present as DIFFUSE GLYCOGEN MASS in young cysts

Question 38

When to report results as E.histolytica or E.dispar

Answer 38

● by microscopy ● by PCR

Question 39

What should be reported if trophozoites contain ingested red blood cells? ● E.histolytica ● E.dispar ●both

Answer 39

E.histolytica

Question 40

What should be reported if trophozoites contain ingested red blood cells? ● E.histolytica ● E.dispar ●both

Answer 40

E.histolytica

Question 41

How should trophozoites be reported if they do not contain ingested red blood cells? ● E.histolytica ● E.dispar ●both

Answer 41

Both