HELP!!!!! Flashcards

1
Q

what are enzymes

A

biological catalysts that lower thee activation energy
by speeding the rate of reaction

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2
Q

explain how an enzyme andd a substrate link together

A

the tertiary structure of the active site is complimentary to the substrate

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3
Q

what is called when an enzyme and substrate join togther

A

enzyme substrate complex

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4
Q

explain the induced fit model

A

a conformational change in tertiary structure of active site.
allows for substrate to fit

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5
Q

explain the effect of temperature on enzyme controlled reactions in the inital stages

A

as the temp increases enzyme and substrate have more kinetic energy
frequency of collisons increases
more succesful enzyme substrate complexes

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6
Q

what does the enzyme and subsrate gain when temp is involved

A

kinetic energy

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7
Q

what happens when the temperature of the enzyme is too high

A

enzyme denatures
breaks H+ bonds
changes the tertiary structure of active sight
so substrate no longer complimentary
no ESC

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8
Q

when do enzymes wwork at their fastest

A

optimum temperature

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9
Q

whaat is the point of saturation/ Vmax
on substrate complexes

A

this is when all the active sites arent readily avaliable as ESC have been formed

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10
Q

what happens when we increase the frequency of substrates

A

greater collisons between them

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11
Q

what is the relationship with the effect of substrate concentration

A

directly proportional

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12
Q

what is the relationship between enzyme concentration on enzymes

A

dirctly proportional

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13
Q

explain the trendd in enzymes from enzyme concentration

A

it increases because there are greater amounts of collisions.
more ESC formed

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14
Q

what happens when the graph plateaus for enzyme concentration

A

substrate aren’t readily avaliabe
so not enough to bind onto enzyme
reaction will no longer spped up

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15
Q

what does a competitive inhibitor do?

A

competes with the substrate in order to bind onto the active site

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16
Q

how can we reduce the effect of a competitive inhibitor

A

by increasing the substrate

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17
Q

why do competitve inhibitors reduce ESC

A

they have a similar shape to the active site
prevents substrate from binding
but no reaction occurs

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18
Q

what do non competitve inhibitors bind onto

A

allosteric site of the enzyme

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19
Q

what does DNA stand for

A

deoxyribonucleic acid

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20
Q

what does RNA stand for

A

ribonucleic acid

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21
Q

what does DNA do

A

holds genetic information

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22
Q

what does RNA do

A

transfers genetic material frrom DNA to ribosomes

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23
Q

how are ribosomes formed

A

from RNA and proteins

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24
Q

what is the monomer for DNA and RNA

A

nucleotides

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25
what does DNA code for
amino acids in primary structure of a protein
26
what forms a part of a nucleotide?
phosphate group pentose sugar nitrogenus bases
27
what is the name of the pentose sugar in DNA
deoxyribose
28
what are the base pairs in DNA
adenine thymmine cytosine guanine
29
what bond is formed durig a condensation reaction betweeen 2 nucleotides
phosphodiester bond (strong covalent)
30
what is the shape of a DNA molecule
double heliz
31
what is a polynucleottide
when many nucleotides are joinedd together
32
what bond holds the complimentary base pairs together
hydrogen bonds
33
how many H+ bonds is present between guanine and cytosine
3
34
how many H+ bonds is present between adenine and thymine
2
35
what is the sugar phospahte backbone
provides structural support where the phosphodiester bond is
36
where is the phosphhodiester bond found
betwen pentose sugar and phosphate group
37
why did scientists not believe that DNA carried any genetic code
because of its simplicity
38
what is the name of the pentose sugar in RNA
ribose
39
what is the name of the nitrogenus containing bases
guanine cytosine adenine uracil
40
what is the name of the nitrogenus containing bases in RNA
guanine cytosine adenine uracil
41
what is the length if RNA
short
42
what is thee role of DNA helicase
breaks the hydrogen bonds between complementary base pairs in the polynucleotide strand
43
what unwinds the double heliex
DNA helicase
44
why are the free DNA nucleotides attracted to the exposed bases on the template strand
complimentary base pairing
45
what is the role of DNA polymerase
cataylses the condensation reaction that forms a phosphodiester bond between adjacent nucleotides
46
what is the name of the model that validated DNA replication
Watson-Crick model
47
what is meant by semi conserving replication
the original strand is conserved
48
what does ATP stand for
adenosine triphosphate
49
what is ATP formed
from a molecule of ribose
50
what is adenine
a nirogenus base
51
what is ATP
nucleotide derivative
52
whaat makes up ATP
adenine 3 phosphate groups pentose sugar--> ribose
53
what chemical proceess is ATP crreated
respiration
54
what enzyme catalyses the hydrolysis of ATP to ne used as energy
ATP hydrolayse
55
what is the equation for the hydrolysis for ATP
ATP + water---> ADP + Pi (energy)
56
what can the inorganic phosphate realsedd during the hydrolysis of ATP be used for
phosphorylate them to make them more reactive
57
what does phosphorylate mean
make other compounds more reactive
58
why is ATP good
immediate form of energy in a small portion(less wasted)
59
why is ATP an immediate source
only one bond is hydrolysed
60
how is ATP resynthesised
condensation between ADP+Pi
61
when is ATP resynthesised
photosynthesis respiration
62
what enzyme cataylses thee condensation reaction between ADP+Pi adesonie diphosphate
ATP synthase
63
what enzyme cataylses thee condensation reaction between ADP+Pi adesonie diphosphate
ATP synthase
64
what is the name of tthe enzymes responsible for the process of semi conservative repication
DNA polymerase DNA helicasse
65
name tthe protein associated with DNA in a chromosome
histone
66
what is the role of a single strand of DNA fragments
a template that determines the order of nucleotides
67
explain a feature of DNA that is important for semi conservative replication
hydrogen bonds are easy to break allows strands to separate 2 strands that act as templates