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Hematology Flashcards

(95 cards)

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0
Q

Type of specimen that has an increase concentration of bilirubin

A

Icteric blood sample

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1
Q

3 phases of hematopoietic development

A

Mesoblastic phase
Hepatic phase
Medullary or myeloid phase

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2
Q

How far does a fire extinguisher must be in every lab?

A

75 ft

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3
Q

A bleach solution with sand

A

Tuberculoidal disinfectant

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4
Q

He noted that carbon dioxide was taken up by the blood from the tissues and discharged in the lungs

A

E.F. Pfluger

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5
Q

What does LE means in LE cell preparation?

A

Lupus erythrematosus

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6
Q

Angle of spreader slide against the first slide

A

30-45 degrees

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7
Q

Define segmenters

A

Thin nuclear filaments that connects 2 lobes

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8
Q

Insufficient staining, prolonged washing time, acidity of stain is too high, and exposure of the stain to acidic cause this staining problem

A

Excessively pink stained

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9
Q

An antiglycolytic agent

A

Sodium fluoride

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10
Q

Optimal concentration of EDTA

A

1.5 mg/ml of blood

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11
Q

Specific gravity of blood

A

1.048-1.066

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12
Q

Used in the preparation of buffy coat for LE cell examination

A

Defibrination

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13
Q

As the cell matures, amount of cytoplasm _____. For as to cell size, it becomes _____

A

Increases. Smaller

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14
Q

Main erythroid organ in the hepatic phase

A

Liver

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15
Q

Stage of erythropoieses wherein fragments of nucleus have been disappear

A

Reticulocyte

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16
Q

N/C ratio of basophilic normoblast

A

6:1

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17
Q

Promoted study of plasma

A

Johannes Muller

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18
Q

Karl Landsteiner contribution

A

Discovered the existence of ABO antigens and proposed 4 major blood types

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19
Q

Anticoagulant of yellow tube

A

Adenine citrine dexthrose and Sodium polyethanol sulfate

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20
Q

Type of AC that causes aggregation of cells

A

Heparin

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21
Q

Define hematopoiesis

A

Formation and development of blood cells in the bone marrow

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22
Q

Stage of erythropoieses in which hemoglobin production starts

A

Rubricyte

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23
Q

Stem cell theory in which each blood cell is derived from its own unique stem cell

A

Polyphyletic theory

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24
Multiple extraction in a single draw from a single patient
Evacuated system
25
Transmigration is faster than other WBCs
Neutrophil
26
``` The following fxns as phagocytes except: Basophil Neutrophil Monocyte Lymphocyte ```
Lymphocyte
27
Established many of the normal limits of the blood composition
Walter Cannon
28
Test done for yellow tubes
Blood cultures
29
Coagulation factor #4
Calcium
30
Type of specimen that has a greater concentration of lipids
Lipemic specimen
31
Main organ in the mesoblastic phase
Mesodermal cells of the yolk sac
32
2 types of agranulocyte
Lymphocyte | Monocyte
33
What basophil produces?
Histamine Heparin Serotonin
34
Technique for recapping needles
Fish out technique
35
Chain of infection for biological hazards
Source Mode of transmission Host
36
In skin puncture, how do we puncture fingertip? Is the lancet parallel or in perpendicular to the fingertip?
In perpendicular
37
What vein in the antecubital fossa must be used in venipuncture?
Median cubital vein
38
Color of serum tubes
Red
39
Needle used in bone marrow aspiration
Jamshidi needle
40
Viscosity of blood compared to water
5x more viscouse
41
Serum contaims fibrinogen whereas plasma does not contain fibrinogen. Is it true or false?
False
42
It destroys histamine and produces anti histamine
Eosinophil
43
When using a plastic test tube for platelet, how does ot affect platelet count? Falsely low or falsely high?
Falsely low
44
Coagulation factor #2
Thrombin
45
Most common site for aspiration
Iliac crest
46
Capillary tube must be filled with blood for at least _______
Two-thirds
47
In order for a fire to occur, these 3 components must be present
Oxygen Source of heat Fuel
48
TRUE or FALSE: A safety practice in the hema lab includes quarterly fire drill
True
49
Blue-stoppered tubes contain anticoagulant which is commonly used in what hematological tests?
Coagulation tests
50
In the hema lab, the majority of specimens are collected in lavender-stoppered tubes. The primary additive in these tubes is:
EDTA
51
Define hematology
Scientific study of the structure and functions of blood in health and in disease
52
Erasistratus said that the cause of disease was due to undigested food
Plethora
53
Anticoagulant in a green-stoppered tubesis also known as the naturally occurring anticoagulant and is referred to as:
Heparin
54
The vein of choice for venipuncture
Median cubital vein
55
Yellow-stoppered tibes are used for
Blood cultures
56
Term used to refer to the measurement of the diameter of the needle
Gauge
57
Anticoagulant for preserving the morphology of blood cells
EDTA
58
To inhibit glycolytic action, this anticoagulant is used and is found in gray-stoppered evacuated tubes
Sodium fluoride
59
Type of microscopy for the performance of manual platelet count
Phase contrast microscopy
60
It makes use of a diluent that fixes and preserves RBC as well as the platelets and preventtheir disintegration; colors the latter light bluish for easy identificatio
Rees-Ecker method
61
Each of the intermediate squares in the central square used in RBC count covers an area of
1/25
62
The total volume used in RBC count is computed by
1/25 sq.mm X 5 X 0.1 mm
63
Most common hemocytometer used in blood cells counting has the ruling of
Neubauer
64
Method of platelet count which makes use of 1% ammonium oxalate in distilled water in system
Brecher Cronkite method
65
Where in the ruled area of the platelets counted following the Brecher Cronkite method?
Entire central large square
66
In routing RBC count, the red cells are counted under HPO in the following number of smallest squares:
80
67
Each of the smallest squars in WBC count measures an area of
1/16
68
Most important consideration of the diluting fluid used in routine RBC count
Isotonic in concentration
69
The diluting fluids used in manual WBC count and PLT count share similarly in
Hypotonic concentration
70
RBC count should be repeated whenever the greatest variation between the R squares exceeds a minimun of
10 cells
71
Hemoglobin that is of highest concentration among newborns
Hb F
72
The Sahli method of hgb determination is based on the conversion of Hb to
Acid hematin
73
Where is hgb produced?
In the nucleated RBCs of the bone marrow
74
In order for oxygen to bind with hgb, iron should be in what state?
Ferrous state
75
What is methemoglobin? A. Hgb in which ferrous state has been oxidized to ferric B. Hgb in which ferric has been oxidized to ferrous
A
76
In the cyanmethgb method, whole blood is mized with Drabkin's rgt which consists of _____ to convert hgb to methgb, and the ____ to convert methgb to cyanmethgb
Potassium ferricyanide; Potassium cyanide
77
Heme is a chemical structure made of porphyrin rings with an ______ atom inserted in the center and is attached to four ____ atoms
Iron; nitrogen
78
The polypepetode chains in one Hb A molecule is composed of how many amino acids
574 amino acids
79
Iron is metabolized in the _____ for the synthesis of heme
Mitochondria
80
Indirect method of Hb determination based on the oxygen content of the blood
Gasometric method
81
Which of the following describes erythropoeitin? A. Renal hormone that regulates marrow RBC prod B. Marrow hormone secreted by developing erythroblasts C. Produced primarily by the liver D. Low levels among individuals living at high altitude
A
82
Cell stage - prod of primary granules ceases and prod od secondary granules commences
Promyelocyte
83
Stage of lymphocytic series seen in the bone marrow, thymus, and secondary lymphoid tissues
Prolymphocyte
84
Method that can be used both in ESR and Hct determination
Wintrobe method
85
Value which is taken from tne separation of erythrocyte from the plasma after controlled compaction and dispersion
ZSR
86
In multiple myeloma, ESR is expected to be abnormally increased or decreased?
Abnormally increased
87
This stage of erythrocytic series is described to be pyknotic erythroblast
Metarubricyte
88
The two stages of granulocytes found in the circulating blood are
Band cells and segmented cell
89
The proliferative compartment of the leukocyte is also known as
Mitotic pool
90
WBC - granules stain red and nucleus stains bluish
Eosinophils
91
WBC - reddish lilac granules with bluish violet nucleus
Neutrophils
92
WBC - cytoplasm faint blue gray tinge with light blue nucleus
Monocytes
93
WBC - bluish cytoplasm with eccentric nucleus
Lymphocytes
94
WBC - granules stain blue or dark bluish purple
Basophils

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