HIS2;

Question 1

41. To excellently fixed intercalated disk of cardiac muscles, which
    of the following may be used?
    a. Hollande’s
    b. Helly’s
    c. Lillie’s B5
    d. Orth’s Fluid

Answer 1

b. Helly’s

Question 2

42. Formol sublimate fixes
    1 Neutral fats
    2 Phospholipids
    3 Lipids
    4 Elastic Fibers

a. 1&3
b. 2&4
c. 1, 2, &3
d. 1, 2, 3, &4

Answer 2

c. 1, 2, &3

Question 3

43. Another aldehyde which has been introduced a mixture of
    glutaraldehyde or formaldehyde

a. Zinc sulfate
b. Glyoxal
c. Acrolein
d. Michel’s solution

Answer 3

c. Acrolein

Question 4

44. Which of the following factors that aside from prolonging
    fixation process can also inactivate enzymes

a. Presence of mucus
b. Cold temperature
c. Presence of blood
d. Presence of fats

Answer 4

b. Cold temperature

Question 5

45. Which of the following is both a nuclear and histochemical
    fixative?

a. Newcomer’s
b. Acetone
c. Formol saline
d. Bouin’s

Answer 5

a. Newcomer’s

Question 6

46. From the choices in #45, which is both microanatomical and
    histochemical?

a. Newcomer’s
b. Acetone
c. Formol saline
d. Bouin’s

Answer 6

c. Formol saline

Question 7

47. What may be added to formaldehyde to prevent decomposition to formic acid?

a. Ethyl alcohol
b. Sodium thiosulfate
c. Lithium carbonate
d. Methanol

Answer 7

d. Methanol

Question 8

48. A formol mercuric chloride solution used for post mortem
    tissues

a. Gendre’s fixative
b. Fleming’s solution
c. Formol corrosive
d. Zenker formol

Answer 8

c. Formol corrosive

Question 9

49. The following fixative has a pH of more than 4.6 except:

a. Bouin’s
b. Helly’s
c. Moller’s
d. Orth’s

Answer 9

a. Bouin’s

Question 10

50. Which of the following fixatives that when used will inhibit
    hematoxylin?

a. Bouin’s
b. Newcomer’s
c. Alcoholic formalin
d. Osmic acid

Answer 10

d. Osmic acid

Question 11

51. Which of the following specimens may require immersion in 4% phenol for 3 days?

1 Uterine
2 Hyperkeratotic skin
3 Cervix
4 Fibroids

a. 1&3
b. 2&4
c. 1,2,3
d. 1,2,3&4

Answer 11

d. 1,2,3&4

Question 12

52. In general, which group of chemical fixatives may cause
    glycogen polarization?

a. Heat fixation
b. Aldehyde fixation
c. Picric acid fixation (Correct: Alcoholic Fixatives)
d. Mercuric chloride fixation

Answer 12

c. Picric acid fixation (Correct: Alcoholic Fixatives)

Question 13

53. Which of the ff. is NOT a commonly used fixative for nucleic
    acid

a. Ethanol
b. Isopropyl alcohol
c. Carnoy’s
d. Methanol

Answer 13

b. Isopropyl alcoho

Question 14

54. Which is the suitable fixation temperature for histochemistry?

a. 0-40 degC
b. 40 degC
c. Room temp
d. 0-4 degC

Answer 14

d. 0-4 degC

Question 15

55. Which of the following concentrations of glutaraldehyde have
    been found to be an ideal concentration for Immune Electron
    Microscopy?

a. 3%
b. 2.5%
c. 0.25%
d. 4%

Answer 15

c. 0.25%

Question 16

56. Using choices in #55, which concentration of glutaraldehyde is
    suited for needle biopsies

a. 3%
b. 2.5% – typo??
c. 0.25%
d. 4%

Answer 16

b. 2.5% – typo??

Question 17

57. Which of the following is recommended for fixation of CNS
    tissues & general post mortem tissues for histochemical
    examination

a. 10% Neutral buffered formalin
b. 10% formol saline
c. Formol corrosive
d. Gendre’s

Answer 17

b. 10% formol saline

Question 18

58. From the choices in #57, which can be used to fix sputum?

a. 10% Neutral buffered formalin
b. 10% formol saline
c. Formol corrosive
d. Gendre’s

Answer 18

d. Gendre’s

Question 19

59. Fixation temperature using autotechnicon

a. 0-40 degC
b. 40 degC
c. Room temp
d. 0-4 degC

Answer 19

b. 40 degC

Question 20

60. The fixative of choice for preserving cell details in tissue
    photography

a. Heat fixation
b. Aldehyde fixation
c. Picric acid fixation
d. Mercuric chloride fixation

Answer 20

d. Mercuric chloride fixation

Question 21

61. What may be added to 10-15% formalin to prevent acid
    reactions due to formic acid formation

a. Calcium carbonate
b. 10% methanol
c. Lithium carbonate
d. Magnesium hydroxide

Answer 21

a. Calcium carbonate

Question 22

62. Which of the following may be added to prevent precipitation
    of formaldehyde to white paraformaldehyde?

a. 10% ethanol
b. 10% methanol
c. 10% butanol
d. 10% isopropanol

Answer 22

b. 10% methanol

Question 23

63. Temperature range for decalcification

a. 37-56 degC
b. 30-37 degC
c. 18-20 degC
d. 18-30 degC

Answer 23

d. 18-30 degC

Question 24

64. Bone decalcification for enzyme histochemistry may be
    satisfactorily achieved by using which of the following decalcifier?

a. 5-10% nitric acid
b. Phloroglucin nitric
c. EDTA
d. Von Ebner’s

Answer 24

c. EDTA

Question 25

65. Which of the following may be added to alcohol bath in dehydration process to soften hard tissues a. Phenol b. HC c. Perenyi’s fluid d. Both A and C

Answer 25

a. Phenol

Question 26

66. Preferred clearing agent for cytological studies a. Clove oil b. Aniline oil c. Castor oil d. Cedarwood oil

Answer 26

d. Cedarwood oil

Question 27

67. Infiltrating method suited for enzyme studies a. Celloidin b. Gelatin c. Paraffin d. Plastic resins

Answer 27

b. Gelatin

Question 28

68. From the choices in #67, which is suited for specimens with large and hollow cavities that tends to collapse a. Celloidin b. Gelatin c. Paraffin d. Plastic resins

Answer 28

a. Celloidin

Question 29

69. Must be done to achieve adequate fixation 1 Use of hypotonic fixative 2 Agitation 3 10:1 ratio of fixative to tissue A. 1&2 B. 1&3 C. 2&3 D. 1,2,3 E. None AB. 1 Only

Answer 29

E. None

Question 30

70. Can be used to fix brain tissues for rabies diagnosis 1. Osmic acid 2. Carnoy’s 3. Acetone A. 1&2 B. 1&3 C. 2&3 D. 1,2,3 E. None AB. 1 Only

Answer 30

C. 2&3

Question 31

71. Can be both fixative and decalcifying agent 1. Acetone (fixative & dehydrating) 2. Ethyl alcohol 3. Methyl alcohol A. 1&2 B. 1&3 C. 2&3 D. 1,2,3 E. None AB. 1 Only

Answer 31

E. None

Question 32

72. Fixatives that will allow excellent trichrome 1. Zenker’s 2. Orth;s 3. Moller’s A. 1&2 B. 1&3 C. 2&3 D. 1,2,3 E. None AB. 1 Only

Answer 32

AB. 1 Only

Question 33

73. Nuclear and Microanatomical 1. Helly’s (pede din sya both) 2. Formol saline (Nucleoproteins & Microanatomical) 3. Acetone (not a microanatomical fixative) A. 1&2 B. 1&3 C. 2&3 D. 1,2,3 E. None AB. 1 Only

Answer 33

A. 1&2

Question 34

74. Embedding mold that when used will yield perfect blocks even without trimming a. Leuckhart’s b. Compound embedding unit c. Peel away mold d. Plastic ice trays

Answer 34

c. Peel away mold

Question 35

75. Plastic resin suited for undecalcified bone a. MMA b. GMA c. Spurr d. Polyester

Answer 35

a. MMA

Question 36

76. Paraffin wax substitute suited for enzyme studies a. Ester wax b. Carbowax c. Paraplast

Answer 36

b. Carbowax

Question 37

77. The most common error in histotechnology is a. Use of concentrated solutions b. Prolonging fixation time c. Cutting tissues more than 5 mm thick d. Prolonging the contact time of fixative to tissues e. None

Answer 37

e. None

Question 38

78. The fixative that can provide improved results in immunohistochemistry a. Clarke’s solution b. Formol acetic alcohol c. Michel solution d. Zinc formalin

Answer 38

d. Zinc formalin

Question 39

79. A traditionally alcohol containing fixative used for frozen sections and smears a. Carnoy’s b. Clarke’s solution c. Formol acetic alcohol d. None of these

Answer 39

b. Clarke’s solution

Question 40

80. Which of the following reagent is commonly used in de-zenkerization a. Alcohol iodine b. Saturated iodine solution c. 70% ethanol d. Saturated picric acid

Answer 40

a. Alcohol iodine