Histology stains Flashcards Preview

CTO Quiz 1 > Histology stains > Flashcards

Flashcards in Histology stains Deck (11):
1

Hematoxylin

Blue
Cation, binds to acidic/anionic/basophilic groups (ie DNA, ribosomes)

2

Eosin

Pink
Collagen, cytoplasm
Eosinophilic, sometimes called "acidophili"

3

PAS (Periodic acid-Schiff's

Recolorizes by oxidation
ie carbohydrates after periodic acid (glycogen, glycoproteins, proteoglycans)

4

Masson's trichrome stain

Nuclei purple-black
Cytoplasm red
Collagen and mucin green

5

Gomori trichrome

Proteins green (collagen, muscle)
Nuclei purple
Lipophillic red
ie muscle biopsies

6

Silver

Delicate structures, many modifications

7

Resorcin-fuchsin

Elastic fibers -> dark blue

8

Wright's blood

Eosin and meth blue
RBCs pink
Nuclei dark blue
Differential of WBCs

9

Giemsa

Similar to Wrights
Chromosome spreads

10

Feulgen reaction

Quantitative DNA -> magenta

11

Metachromasia

Substrates change stain (ie GAGs take toluidine blue -> red)