HISTOPATH LAB (ADH- MNT-STN)

Question 1

is a substance which can be smeared onto the slide so that the sections
stick well to the slides

Answer 1

ADHESIVE

Question 2

Slides must always be __________ and _______
and stored and handled correctly

Answer 2

grease, dust-free

Question 3

If slides are not stored properly, _______ and ________ grows which causes false-positive results after gram
staining

Answer 3

bacteria and fungi

Question 4

Most commonly used adhesive

Answer 4

ALBUMIN

Question 5

Albumin is prepared through

Answer 5

• mixing equal parts of glycerin, distilled water, and white of eggs
• then filtered through a coarse filter
• and a crystal of Thymol is added.

Question 6

One disadvantage of using albumin

Answer 6

it retains some of the stains and gives a dirty
background

Question 7

Meyer’s egg albumin formula

Answer 7

• Egg white 50cc
• Glycerin 50cc
• Filter and add about 100 mg Crystals of thymol

Question 8

Added to adhesive to prevent the growth of molds

Answer 8

Thymol

Question 9

Disadvantage of Meyer’s Egg Albumin

Answer 9

Too thick adhesive may take up stains causing a color blue background.

Question 10

How to remedy the disadvantage of Meyer’s Egg Albumin

Answer 10

Prepare slides again.

Question 11

usually a syrupy fluid
applied between the section and the coverslip
after staining, setting the section firmly
preventing the movement of the coverslip

Answer 11

mounting medium

Question 12

it bonds specimen, slides,
and the coverslip together with a clear durable film

Answer 12

mounting medium

Question 13

Mounting prevents _______ and _________

Answer 13

Oxidation, scratches

Question 14

refractive index of tissue

Answer 14

1.515

Question 15

It is a transparent, almost colorless oleoresin
that adheres firmly to glass and sets to a hard
consistency without granulation

Answer 15

Canada Balsam

Question 16

Canada Balsam RI value

Answer 16

1.524

Question 17

Canada Balsam recommended for whole
mounts and for thick sections because it _______________________

Answer 17

does not shrink much

Question 18

Disadvantage of Canada Balsam

Answer 18

Expensive

Question 19

Canada Balsam is sourced from ________________

Answer 19

Canadian Tree, Abus Balsamea

Question 20

CHARACTERISTICS OF A
GOOD MOUNTING MEDIUM

Answer 20

1. be colorless and transparent.
2. be freely miscible with xylene and toluene.
3. not dry to a non-stick consistency and harden
   relatively quickly.
4. protect the section from physical damage and
   chemical activity (oxidation and changes in pH).
5. be resistant to contamination (particularly
   microorganism growth).
6. not cause shrinkage and distortion of tissues.
7. not leach out any stain or affect staining
8. not change in color or pH.
9. be compatible with the adhesive in use.
10. set without crystallizing, cracking, or shrinking
    (or otherwise deform the tissue being mounted)
    and not react with leach or induce fading in
    stains and reaction products (including those
    from enzyme histochemical, hybridization—).

Question 21

The process of sealing the margins of the
coverslip to:
★ prevent the escape of fluid or semi-fluid
mounts and evaporation of mountant
★ fix the coverslip in place
★ prevent sticking of the slides upon
storage.

Answer 21

Ringing

Question 22

easier to use and less bubble formation

Answer 22

EPON: epoxy resin

Question 23

The process is whereby the tissue components
are made visible with a dye or staining solution

Answer 23

STAINING

Question 24

main principle of Staining

Answer 24

Done to visualize cells

Question 25

giving color to the section by using aqueous solution or alcoholic dyes solutions.

Answer 25

Direct Staining

Question 26

action of the dye is intensified by adding another agent or a mordant or an accentuator.

Answer 26

Indirect Staining

Question 27

Example of Indirect Staining

Answer 27

phenol, potassium hydroxide

Question 28

tissue elements are stained in a definitive sequence.

Answer 28

Progressive Staining

Question 29

the tissue is first overstained to obliterate the cellular details, excessive stains is removed or decolorized from unwanted parts.

Answer 29

Regressive Staining

Question 30

use more than one chemical stain to differentiate between various microorganisms or structures.

Answer 30

Differential Staining

Question 31

Cornerstone of tissue-based diagnosis

Answer 31

H&E STAINING

Question 32

H&E STAINING is a type of ________ staining

Answer 32

Regressive

Question 33

H&E STAINING Nuclei= _______ Cytoplasm and other organelles= ________

Answer 33

Blue, Pink to Red

Question 34

HEMATOXYLIN came from _____________________ with its active agent called ________

Answer 34

Hematoxylin campechianum (Mexican Tree), hematin

Question 35

STAINING PROCESS IN PROPER ORDER with TIME

Answer 35

1) XYLENE 1- 3mins 2)XYLENE 2- 3mins 3) XYLENE 3- 3mins 4) ABS OH- 3mins 5) 95% OH- 3mins 6) 80% OH- 3mins 7) 1-WATER- 10dips 8) HEMATOXYLIN- 30mins 9) 2-WATER- 10dips 10) ACID OH- 1dip 11) 3-WATER- 10dips 12) AMMONIA WATER- 5secs 13) 4-WATER- 10dips 14) EOSIN- 7dips 15) 1-95% OH- 10dips 16) 2-95% OH- 10dips 17) 1-ABS OH- 10dips 18) 2-ABS OH- 10dips 19) XYLENE 1- 10dips 20) XYLENE 2- 10dips 21) XYLENE 3- 10dips