Histopathology And MT Laws

Question 1

Accidental skin contact with hazardous chemicals; wash hands with water for

Answer 1

15 to 30 mins

Question 2

Process done by the automatic tissue processor

Answer 2

Fixation, dehydration, clearing and infiltration

Question 3

Stations 1-2 :
Stations 3-6 :
Stations 7-8 :
Stations 9-10 :
Stations 11-12 :

Answer 3

10% formalin
Ascending grades of ethyl alcohol 70- 95%
2 changes of acetone
2 changes of chooroform or xylol
2 changes of liquid paraffin

Question 4

Renoval of nicks

Answer 4

Honing

Question 5

Removal of burrs

Answer 5

Stropping

Question 6

Nuclear and histochemical fixative

Answer 6

Newcomer’s fixative

Question 7

Most common metallic fixative

Answer 7

Mercurial fixative

Question 8

Fixative for tissue photography

Answer 8

Mercurial fixative

Question 9

Fixative for small tissue fragements

Answer 9

Oicric acid; yellow color to locate tissue easier

Question 10

Excellent fixative for preservkng soft amd delicate structures

Answer 10

Bouin’s solution

Question 11

Picric acid fixative

Answer 11

Bouin’s, Brasil’s

Question 12

Fixative for electrom microscopy

Answer 12

Glutaraldehyde, osmium tetroxide

Question 13

Air-filled lungs may float on fixative what should be done

Answer 13

Organ may be covered with several gauze to maintain it under surface

Question 14

Decalcifying agent that contains HCl

Answer 14

Von Ebner’s (HCl, NaCl, d. Water)

Question 15

Embeddiing medium for electron microscopy

Answer 15

Plastic medium

Question 16

Double embedding

Answer 16

Infiltrated with celloidin,
Embedded with paraffin

Question 17

Fastest embeding method

Answer 17

Vacuum embedding

Question 18

Quenching temperature in freeze-drying

Answer 18

-160 to -180 degree celsius

Question 19

Dessication/ sublimation temp for freeze drying

Answer 19

-30 to -40 degree celsius

Question 20

Warer must be removed by heating the waxmat what temp

Answer 20

100 to 105 degree celsius

Question 21

Paraffin wax oven temp

Answer 21

55 to 60 or 2 to 5 degree celsius mp wax

Question 22

Floatation water bath temp

Answer 22

45 - 50 degree celsius or 6 to 10 degree celsius below mp

Question 23

Simplest microtome, Paldwell trefall

Answer 23

Rocking microtome

Question 24

Most common microtome, Minot

Answer 24

Rotaty microtome

Question 25

Most dangerous type of microtome, Adams

Answer 25

Sliding microtome

Question 26

Releases CO2, Queckett

Answer 26

Freezing microtome

Question 27

Electrically driven refrigerated chamber, where microtome, knife, specimen and atmosphere are kept at rhe same temperature

Answer 27

Cryostat

Question 28

Temp of cryostat

Answer 28

-20

Question 29

Bevel angle of microtome

Answer 29

27 to 32 degree

Question 30

Clerance angle of microtome

Answer 30

0 to 15 degree angle 5 to 10 degree angle to prevent uneven sections or alternate thick and thin section

Question 31

Regressive staining, it involves a differentiation step (acid alcohol)

Answer 31

Manual H and E staining

Question 32

Eosin, phloxine, picric acid, orange G, Rose Bengal, light green, lissamine green are examples of

Answer 32

Cytoplasmic stains

Question 33

Neutral red, safranin, carmine, hematoxyljn, methylene blue, toluidine blue, celestine blue are what kind of stain

Answer 33

Nuclear stain

Question 34

Specific reaction for DNA

Answer 34

Feulgen technique

Question 35

What is the DNA and RNA color in methyl green

Answer 35

DNA- green or blue green RNA- rose red

Question 36

Color of acid mucopolysaccharide, fungi, background in fluorescent acridine irange technique

Answer 36

Acid mucopolysaccharide- black Fungi- greenish red fluorescence Background- reddish orange fluorescence

Question 37

Histones and protamines in alkaline fast green

Answer 37

Green

Question 38

Stain for the basement membrane

Answer 38

PAS, azocarmine

Question 39

Demonstration of muscle striations

Answer 39

Mallory’S PTAH

Question 40

Stain for helicobacter pylori

Answer 40

Toluidene blue, cresyl violet acetate

Question 41

Oldest stain, glycogen mahogany brown

Answer 41

Iodine

Question 42

Reticulin fibers are stained black

Answer 42

Gomori’s stain

Question 43

Best location for specimen of vaginal smears

Answer 43

Upper lateral third of the vaginal wall

Question 44

Respiratory tract specimens are

Answer 44

Sputum, bromchoalveolar lavage/ bronchial washing, Bronchial brushing

Question 45

Gastrointestinal specimens are

Answer 45

Gastric lavage, gastric brush and FNA

Question 46

If delayed, CSF specimen for cytology should be

Answer 46

Refrigerated

Question 47

Reversible change from one adult dell type to another

Answer 47

Metaplasia

Question 48

Most of the nuclei of females exhibit conglomerqtion ir chromatin, demomstrated in the smears from buccal or vaginal mucosa

Answer 48

Barr bodies, XX chromosomes

Question 49

Autopsy where organs are removed one by one

Answer 49

Virchow

Question 50

Autopsy which involves insitu dissection

Answer 50

Rokitansky

Question 51

En bloc autopsy

Answer 51

Ghon

Question 52

En masse autopsy

Answer 52

Letulle

Question 53

Anti rabies act of 2007

Answer 53

RA 9482